Expression of angiogenesis‐ and apoptosis‐related genes in chorionic villi derived from recurrent pregnancy loss patients

Choi, Heekyung; Choi, Bum Chae; Lee, Sook‐Hwan; Kim, Jeong Wook; Yul, Kwang; Baek, Kwang‐Hyun

doi:10.1002/mrd.10331

Cited by 98 publications

(70 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kwang-Hyun Baek compared the gene expression level of angiogenesis-related genes in chorionic villi of RSA patients with those of normal controls (Choi et al 2003). The result indicated that the lower expression of angiogenesis-related genes is associated with RSA (Choi et al 2003). In our study, high expression of hsa-miR-519a and hsa 520h in decidua can downregulate matrix metalloproteinase 2 (MMP2; Supplementary Table 3).…”

Section: Discussionmentioning

confidence: 59%

“…Previous study has demonstrated that the angiogenesis in NP is very critical (Reynolds et al 1992, Chatzaki et al 2001. Kwang-Hyun Baek compared the gene expression level of angiogenesis-related genes in chorionic villi of RSA patients with those of normal controls (Choi et al 2003). The result indicated that the lower expression of angiogenesis-related genes is associated with RSA (Choi et al 2003).…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Dong¹,

Zhang²,

Xia³

et al. 2014

Reproduction

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 93%

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Dong¹,

Zhang²,

Xia³

et al. 2014

Reproduction

View full text Add to dashboard Cite

show abstract

“…11 Abnormal expression of pro-and antiapoptotic molecules have been identified in tissues from patients with RPL, and higher expression of FAS and FAS-L genes have been found in chorionic villi from patients with RPL. 8,12 Single-nucleotide polymorphisms (SNPs) have been identified in the promoter regions of FAS-FAS-L genes. Experimental results suggest that a polymorphism at the À670 position (A to G) of the FAS gene leads to decreased FAS production, while a polymorphism at the À844 position (C to T) of the FAS-L gene leads to increased expression of this molecule.…”

Section: Introductionmentioning

confidence: 99%

FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

et al. 2013

View full text Add to dashboard Cite

We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case-control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.

show abstract

“…Earlier studies have reported alterations in the expression of several MMP genes in women with IRSA and their spontaneously aborted conceptuses compared to women with normal pregnancies, which can lead to an altered ECM turnover [10,12,[17][18][19][20][21][22][23][24]. In addition, we previously evaluated the potential association of MMP1, 2, 3, and 9 functional single nucleotide polymorphisms (SNP) with IRSA and found a statistically significant increased frequency of the MMP2 -735 CT and MMP9 -1562 CC genotypes in IRSA women compared to controls, suggesting that these might be risk genotypes for IRSA [25].…”

Section: Introductionmentioning

confidence: 99%

Functional single nucleotide polymorphisms of matrix metalloproteinase 7 and 12 genes in idiopathic recurrent spontaneous abortion

Barišić

Pereza

Hodžić

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to investigate the potential association of matrix metalloproteinase 7 (MMP7) -181 A/G and MMP12 -82 A/G functional single nucleotide polymorphisms (SNP) with idiopathic recurrent spontaneous abortion (IRSA) in Slovenian reproductive couples. Methods A case-control study was conducted on 149 couples with 3 or more consecutive idiopathic spontaneous pregnancy loses and 149 women and men with at least 2 live births and no history of pregnancy complications. Genotyping of MMP7 -181 A/G and MMP12 -82 A/G SNPs was performed using polymerase chain reaction and restriction fragment length polymorphism methods. Results There were no statistically significant differences in the distribution of MMP7 -181 A/G and MMP12 -82 A/G genotype, allele, or haplotype frequencies between IRSA patients and controls, as well as patients' primary and secondary IRSA. We also found no association of MMP7 -181 A/G and MMP12 -82 A/G genotypes, alleles, and haplotypes with IRSA. Conclusions We found no evidence to support the association between IRSA and MMP7 -181 A/G and MMP12 -82 A/G SNPs in Slovenian reproductive couples.

show abstract

Expression of angiogenesis‐ and apoptosis‐related genes in chorionic villi derived from recurrent pregnancy loss patients

Cited by 98 publications

References 42 publications

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

Functional single nucleotide polymorphisms of matrix metalloproteinase 7 and 12 genes in idiopathic recurrent spontaneous abortion

Contact Info

Product

Resources

About