Expression of an immunoglobulin heavy chain transgene in macrophage as well as lymphocyte lineagesin vivo

Pasare, Chandrashekhar; Noggle, Scott; Entringer, Maureen A.; A, Heinzelmann; Bansal, Pratima; George, Anna; Bal, Vineeta; Rath, Satyajit; Durdik, Jeannine M.

doi:10.1002/(sici)1521-4141(199904)29:04<1219::aid-immu1219>3.0.co;2-h

Cited by 2 publications

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“…The same recombinases control both T‐ and B‐cell development, and T‐cell receptors can rearrange in B cells 35 . There are numerous examples in which partial or complete immunoglobulin genes are rearranged in normal T cells, 36–39 in malignant lymphocytes 40–42 and other leucocytes 43 . It is now generally accepted that the pathway to T‐ rather than B‐cell development is controlled by Notch I, 44 presumably because Notch I–ligand interactions control the E‐protein family of transcription factors 45 .…”

Section: Discussionmentioning

confidence: 99%

“…35 There are numerous examples in which partial or complete immunoglobulin genes are rearranged in normal T cells, [36][37][38][39] in malignant lymphocytes [40][41][42] and other leucocytes. 43 It is now generally accepted that the pathway to T-rather than B-cell development is controlled by Notch I, 44 presumably because Notch I-ligand interactions control the E-protein family of transcription factors. 45 In fact, the under-expression of Notch I can result in a 20-fold increase of thymic B cells, 44 and B cells instead of T cells develop in the thymus of mice with inactivated Notch I.…”

Section: Discussionmentioning

confidence: 99%

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Antibody repertoire development in fetal and neonatal piglets. XI. The thymic B‐cell repertoire develops independently from that in blood and mesenteric lymph nodes

et al. 2005

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Summary The origin and function of thymic B cells is currently unresolved. In the present study we compared VH gene repertoire diversification in >3500 cloned VDJs (from 11 animals at three time‐points, using three to five animals per time‐point) that were expressed with immunoglobulin (Ig)M, IgD, IgG, IgA and IgE in thymus, mesenteric lymph nodes (MLN) and peripheral blood B cells (PBB) of newborn piglets and 5‐week‐old isolator piglets maintained germfree (GF) or colonized with Escherichia coli. The results showed that the repertoire expressed with IgM, IgD, IgG and IgA in PBB and MLN remained polyclonal, undiversified and unselected in piglets maintained GF for 5 weeks, that age and colonization resulted in significant repertoire diversification of IgG and IgA in the MLN and of IgG in blood, that the thymic B‐cell repertoire was polyclonal, unaffected by colonization and showed no clonal selection in any isotype, and that the thymic IgA and IgE repertoires were more diverse at birth than the repertoire of any isotype in MLN or PBB. IgD was seldom recovered from the PBB of newborn piglets or at any time‐point in thymus, but was recovered in the MLN of all 11 animals examined. The IgD and IgM repertoires in all tissues remained polyclonal and unselected, although VH usage by IgD transcripts did not always parallel that of IgM in the same tissue. Therefore, isotype‐switched B cells in the thymic medulla cannot be accounted for by immigration of B cells diversified by colonization of the gut, and thymic B cells undergo switch recombination and repertoire diversification before birth without clonal selection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antibody repertoire development in fetal and neonatal piglets. XI. The thymic B‐cell repertoire develops independently from that in blood and mesenteric lymph nodes

et al. 2005

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Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis

et al. 2012

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In this study we were interested in identification of new markers of chicken response to Salmonella Enteritidis infection. To reach this aim, gene expression in the spleens of naive chickens and those intravenously infected with S. Enteritidis with or without previous oral vaccination was determined by 454 pyrosequencing of splenic mRNA/cDNA. Forty genes with increased expression at the level of transcription were identified. The most inducible genes encoded avidin (AVD), extracellular fatty acid binding protein (EXFABP), immune responsive gene 1 (IRG1), chemokine ah221 (AH221), trappin-6-like protein (TRAP6) and serum amyloid A (SAA). Using cDNA from sorted splenic B-lymphocytes, macrophages, CD4, CD8 and γδ T-lymphocytes, we found that the above mentioned genes were preferentially expressed in macrophages. AVD, EXFABP, IRG1, AH221, TRAP6 and SAA were induced also in the cecum of chickens orally infected with S. Enteritidis on day 1 of life or day 42 of life. Unusual results were obtained for the immunoglobulin encoding transcripts. Prior to the infection, transcripts coding for the constant parts of IgM, IgY, IgA and Ig light chain were detected in B-lymphocytes. However, after the infection, immunoglobulin encoding transcripts were expressed also by T-lymphocytes and macrophages. Expression of AVD, EXFABP, IRG1, AH221, TRAP6, SAA and all immunoglobulin genes can be therefore used for the characterization of the course of S. Enteritidis infection in chickens.

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Expression of an immunoglobulin heavy chain transgene in macrophage as well as lymphocyte lineagesin vivo

Cited by 2 publications

References 46 publications

Antibody repertoire development in fetal and neonatal piglets. XI. The thymic B‐cell repertoire develops independently from that in blood and mesenteric lymph nodes

Antibody repertoire development in fetal and neonatal piglets. XI. The thymic B‐cell repertoire develops independently from that in blood and mesenteric lymph nodes

Characterization of Chicken Spleen Transcriptome after Infection with Salmonella enterica Serovar Enteritidis

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