Expression of an endo α-1, 3-Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight

Kumar, Rahul; Kumari, Kumkum; Hembram, Krushna Chandra; Kandha, Laxman; Bindhani, Birendra Kumar

doi:10.1007/s13562-018-0465-7

Cited by 16 publications

(5 citation statements)

References 18 publications

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“…T. harzianum is indicated as a biocontrol agent in numbers of the plant species. Begum et al (2010) reported the application of T. harzianum IMI 392432 to suppress Alternaria fruit rot disease in chili, T. harzianum UBSTH-501 induced systemic resistance on the spot blotch pathogen Bipolaris sorokiniana in wheat (Singh et al 2019) while T. harzianum WKY1 controlled anthracnose disease in sorghum (Saber et al 2017) and provided resistance against sheath blight in rice (Kumar et al 2019). Since the biological control of fungal pathogens is an ecofriendly approach for crop protection, TH-IS005-12 could be applied as a fungicidal protective agent for healthy and organic IRG seeds production.…”

Section: Resultsmentioning

confidence: 99%

Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage

Banjac¹,

Stanisavljević²,

Dimkić³

et al. 2021

PLANT SOIL ENVIRON

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Potential of Trichoderma harzianum IS005-12 (TH-IS005-12) to promote seed germination and seedling growth of Italian ryegrass (IRG) forage was evaluated in vitro. Non-desiccated seeds and those pre-harvestly desiccated with total herbicide were treated 25 days (freshly harvested) and 178 days after harvest (mature) with TH-IS005-12 spore suspensions at 0 (T0), 1.8 × 10<sup>7</sup> (T1) and 1.2 × 10<sup>9</sup> (T2) spore/mL. TH-IS005-12 promoted the early and final germination and seedling growth in all non-desiccated and desiccated, freshly harvested as well as mature IRG seeds. It was more effective in pre-harvestly desiccated freshly harvested seeds where T2 treatment increased final germination rate for 24%, root number per seedling 1.6-fold and seedling vigour 1.9-fold compared to the untreated control. Moreover, TH-IS005-12 showed an inhibitory activity against seedborne fungi Alternaria alternata and A. ventricosa suppressing their growth in vitro by 82% and 77%, respectively.

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Section: Resultsmentioning

confidence: 99%

Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage

Banjac¹,

Stanisavljević²,

Dimkić³

et al. 2021

PLANT SOIL ENVIRON

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“…Further, there is some evidence that α-1,3-glucanases prevent plant detection of β -1,3 - glucan in in invading pathogens, negatively impacting plant defensive responses. Transgenic rice plants expressing bacterial (105) or fungal (106) α-1,3-glucanases demonstrate protection against fungal pathogens. Thus, it is also possible that these enzymes are important in plant-fungal mutualism and beneficial to plants (107, 108).…”

Section: Discussionmentioning

confidence: 99%

Broad geographical and ecological diversity from similar genomic toolkits in the ascomycete genusTetracladium

Anderson

Marvanová

2020

Preprint

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18The ascomycete genus Tetracladium is best known for containing aquatic hyphomycetes, which are 19 important decomposers in stream food webs. However, some species of Tetracladium are thought 20 to be multifunctional and are also endobionts in plants. Suprisingly, Tetracladium sequences are 21 increasingly being reported from metagenomics and metabarcoding studies of both plants and soils 22 world-wide. It is not clear how these sequences are related to the described species and little is 23 known about the non-aquatic biology of these fungi. Here, the genomes of 24 Tetracladium strains, 24 including all described species, were sequenced and used to resolve relationships among taxa and to 25 improve our understanding of ecological and genomic diversity in this group. All genome-sequenced 26 Tetracladium fungi form a monophyletic group. Conspecific strains of T. furcatum from both aquatic 27 saprotrophic and endobiont lifestyles and a putative cold-adapted clade are identified. Analysis of 28 ITS sequences from water, soil, and plants from around the world reveals that multifunctionality 29 may be widespread through the genus. Further, frequent reports of these fungi from extreme 30 environments suggest they may have important but unknown roles in those ecosystems. Patterns of 31 predicted carbohydrate active enzymes (CAZyme) and secondary metabolites in the Tetracladium 32 genomes are more similar to each other than to other ascomycetes, regardless of ecology, 33 suggesting a strong role for phylogeny shaping genome content in the genus. Tetracladium genomes 34 are enriched for pectate lyase domains (including PL3-2), GH71 α-1,3-glucanase domains and CBM24 35 α-1,3-glucan/mutan binding modules, and both GH32 and CBM38, inulinase and inulin binding 36 modules. These results indicate that these fungi are well-suited to digesting pectate and pectin in 37 leaves when living as aquatic hyphomycetes, and inulin when living as root endobionts. Enrichment 38 for α-1,3-glucanase domains may be associated with interactions with biofilm forming 39 microorganisms in root and submerged leaf environments. 42 important decomposers in stream ecosystems. The group is polyphyletic, its members are classified 43 in various orders and families of Ascomycetes and Basidiomycetes. These fungi thrive on leaves and 44 other plant debris that enter streams and other water bodies from terrestrial plants. In the process, 45 carbon and nutrients from recalcitrant plant compounds, including cellulose and lignin, become 46 accessible to diverse consumers in the stream food web (reviewed in (1, 2)). Up to 99% of the carbon 47 in some streams enters as plant debris that is largely inaccessible to aquatic consumers without the 48 degradative activity of these fungi (3, 4). Aquatic hyphomycete adaptations to stream environments 49 include the production of asexual propagules (conidia) while under water. These spores are often 50 branched, typically tetraradiate, or sigmoid, readily detachable from the fungus (e.g. from 51 conidiophores) and passi...

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“…Previous studies revealed that the defense-related response played an essential role in improving the disease resistance of plants. GLU enhances the tolerant of rough lemon rootstock against foot rot (Sandhu et al, 2019), improves the inhibition of rice against Rhizoctonia solani (Kumar et al, 2018), reduces the rice sheath blight (Kaur et al, 2021), and confers tea tree resistance against blister blight disease (Singh et al, 2018). POD is involved in the resistance response of coffee varieties to Colletotrichum kahawae (Diniz et al, 2019), potato to P. infestans , Arabidopsis thaliana to Botrytis cinerea, Colletotrichum higginsianum, and Pectobacterium carotovorum (Zhao et al, 2019), Citrus sinensis to citrus bacterial canker (Li et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits

et al. 2022

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Apple ring rot caused by Botryosphaeria dothidea is prevalent in main apple-producing areas in China, bringing substantial economic losses to the growers. In the present study, we demonstrated the inhibitory effect of dimethyl trisulfide (DT), one of the main activity components identified in Chinese leek (Allium tuberosum) volatile, on the apple ring rot on postharvest fruits. In in vitro experiment, 250 μL/L DT completely suppressed the mycelia growth of B. dothidea. In in vivo experiment, 15.63 μL/L DT showed 97% inhibition against the apple ring rot on postharvest fruit. In addition, the soluble sugar content, vitamin C content, and the soluble sugar/titratable acidity ratio of the DT-treated fruit were significantly higher than those of the control fruit. On this basis, we further explored the preliminary underlying mechanism. Microscopic observation revealed that DT seriously disrupted the normal morphology of B. dothidea. qRT-PCR determination showed the defense-related genes in DT-treated fruit were higher than those in the control fruit by 4.13–296.50 times, which showed that DT inhibited apple ring rot on postharvest fruit by suppressing the growth of B. dothidea, and inducing the defense-related genes in apple fruit. The findings of this study provided an efficient, safe, and environment-friendly alternative to control the apple ring rot on apple fruit.

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Expression of an endo α-1, 3-Glucanase gene from Trichoderma harzianum in rice induces resistance against sheath blight

Cited by 16 publications

References 18 publications

Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage

Trichoderma harzianum IS005-12 promotes germination, seedling growth and seedborne fungi suppression in Italian ryegrass forage

Broad geographical and ecological diversity from similar genomic toolkits in the ascomycete genusTetracladium

Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits

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