Expression of an Arabidopsis Ca2+/H+ antiporter CAX1 variant in petunia enhances cadmium tolerance and accumulation

Wu, Qingyu; Shigaki, Toshiro; Williams, Kimberly A.; Han, Jeung-Sul; Kim, Chang Kil; Hirschi, Kendal D.; Park, Sunghun

doi:10.1016/j.jplph.2010.06.005

Cited by 82 publications

(36 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAX2 and CAX4; Hirschi et al, 2000;Mei et al, 2009). Furthermore, a mutant of an activated N-terminal form of AtCAX1 that differs by one amino acid in the selectivity domain, named CAXcd, showed high apparent Cd transport , and petunia (Petunia hybrida) plants expressing this CAXcd exhibited greater Cd accumulation than control plants along with fewer toxicity symptoms (Wu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Among them, four genes showed gene ontology annotation associated with Cd response or metal ion transport (Supplemental Table S1). CAX1 was selected for additional studies based on (1) current literature suggesting a role for CAXs in metal transport in the vacuole (Hirschi et al, 2000;Shigaki et al, 2005;Koren'kov et al, 2007;Mei et al, 2007;Wu et al, 2011) and (2) expression analysis of BC1 Ah 3 Alp individuals displaying different alleles at the CdTol2 locus. Indeed, BC1 individuals with the Ah allele at the CdTol2 locus showed significantly higher CAX1 expression levels in shoots than the ones displaying the Alp allele (Fig.…”

Section: Marker Densification In the Cdtol2 Region And Identificationmentioning

confidence: 99%

See 1 more Smart Citation

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

et al. 2015

View full text Add to dashboard Cite

Arabidopsis halleri is a model species for the study of plant adaptation to extreme metallic conditions. In this species, cadmium (Cd) tolerance seems to be constitutive, and the mechanisms underlying the trait are still poorly understood. A previous quantitative trait loci (QTL) analysis performed on A. halleri 3 Arabidopsis lyrata backcross population1 identified the metal-pump gene Heavy Metal ATPase4 as the major genetic determinant for Cd tolerance. However, although necessary, Heavy Metal ATPase4 alone is not sufficient for determining this trait. After fine mapping, a gene encoding a calcium 2+ /hydrogen + antiporter, cation/hydrogen + exchanger1 (CAX1), was identified as a candidate gene for the second QTL of Cd tolerance in A. halleri. Backcross population1 individuals displaying the A. halleri allele for the CAX1 locus exhibited significantly higher CAX1 expression levels compared with the ones with the A. lyrata allele, and a positive correlation between CAX1 expression and Cd tolerance was observed. Here, we show that this QTL is conditional and that it is only detectable at low external Ca concentration. CAX1 expression in both roots and shoots was higher in A. halleri than in the close Cd-sensitive relative species A. lyrata and Arabidopsis thaliana. Moreover, CAX1 loss of function in A. thaliana led to higher Cd sensitivity at low concentration of Ca, higher sensitivity to methylviologen, and stronger accumulation of reactive oxygen species after Cd treatment. Overall, this study identifies a unique genetic determinant of Cd tolerance in the metal hyperaccumulator A. halleri and offers a new twist for the function of CAX1 in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Marker Densification In the Cdtol2 Region And Identificationmentioning

confidence: 99%

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The utility of CAX engineering is demonstrated when a CAX with higher Cd 2+ transport rates was over‐expressed in petunia ( Petunia × hybrida ) plants to confer increased tolerance to the metal (Wu et al . 2011). Improved sequestration of metals such as Cd 2+ in the root tissues of plants can reduce Cd 2+ levels in edible tissues, as Cd 2+ exposure is a major hazard to human health (Nawrot et al .…”

Section: The Cax Transporters In Biotechnologymentioning

confidence: 99%

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

2016

View full text Add to dashboard Cite

Cation/proton exchangers (CAXs) are a class of secondary energised ion transporter that are being implicated in an increasing range of cellular and physiological functions. CAXs are primarily Ca2+ efflux transporters that mediate the sequestration of Ca2+ from the cytosol, usually into the vacuole. Some CAX isoforms have broad substrate specificity, providing the ability to transport trace metal ions such as Mn2+ and Cd2+, as well as Ca2+. In recent years, genomic analyses have begun to uncover the expansion of CAXs within the green lineage and their presence within non‐plant species. Although there appears to be significant conservation in tertiary structure of CAX proteins, there is diversity in function of CAXs between species and individual isoforms. For example, in halophytic plants, CAXs have been recruited to play a role in salt tolerance, while in metal hyperaccumulator plants CAXs are implicated in cadmium transport and tolerance. CAX proteins are involved in various abiotic stress response pathways, in some cases as a modulator of cytosolic Ca2+ signalling, but in some situations there is evidence of CAXs acting as a pH regulator. The metal transport and abiotic stress tolerance functions of CAXs make them attractive targets for biotechnology, whether to provide mineral nutrient biofortification or toxic metal bioremediation. The study of non‐plant CAXs may also provide insight into both conserved and novel transport mechanisms and functions.

show abstract

“…Correspondingly, these CAXs improve Ca 2+ accumulation (Hirschi, 1999), enhance tolerance to Cd 2+ (Korenkov et al, 2007a, b;Wu et al, 2011), and Li + and Na + (Luo et al, 2005) when expressed constitutively in plant tissues. In addition, some of CAXs are also involved in plant various stresses responses, including low temperatures, dehydration and high salt (Knight et al, 1996;Pittman et al, 2009;Han et al, 2011).…”

Section: +mentioning

confidence: 99%

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

Li¹,

Zhou²,

Zheng³

et al. 2017

IJAB

View full text Add to dashboard Cite

The CAX (Calcium Exchanger) genes constitute a set of signal transductors that are important for plant growth and adaptation to environmental stresses, especially to low temperature. CbCAX51, a Ca 2+ /H + exchanger was cloned form Capsella bursa-pastoris, has been reported as a cold inducible gene. Here we showed that the transcriptional level of CbCAX51 in leaf and stem was higher than that in roots. Transcript of CbCAX51 was continuously up-regulated by Ca 2+ and Cu 2+, and transiently up-regulated by Zn 2+ and Li + , cold stress, GA and ABA. The CbCAX51 protein was predicted to be localized in vacuolar by PSORT to Plant-mPLos, which was further confirmed by the subcellular detection of CbCAX51-GFP fusion protein. When CbCAX51 was transformed into the cold-sensitive plant tobacco, it conferred cold tolerance in tobacco seedlings based on physiological studies. Likewise, overexpression of CbCAX51 can activated some cold responsive genes in transgenic tobacco. Taken together, CbCAX51 is involved in the cold signal transduction and modulation of downstream components to enhance cold tolerance in tobacco.

show abstract

Expression of an Arabidopsis Ca2+/H+ antiporter CAX1 variant in petunia enhances cadmium tolerance and accumulation

Cited by 82 publications

References 41 publications

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

Contact Info

Product

Resources

About

Expression of an Arabidopsis Ca2+/H+ antiporter CAX1 variant in petunia enhances cadmium tolerance and accumulation

Cited by 82 publications

References 41 publications

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

CATION EXCHANGER1 Cosegregates with Cadmium Tolerance in the Metal Hyperaccumulator Arabidopsis halleri and Plays a Role in Limiting Oxidative Stress in Arabidopsis Spp.

CAX‐ing a wide net: Cation/H+ transporters in metal remediation and abiotic stress signalling

Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants

Contact Info

Product

Resources

About

CAX‐ing a wide net: Cation/H⁺ transporters in metal remediation and abiotic stress signalling