Expression of an aquaporin at night in relation to the growth and root water permeability in barley seedlings

Aquaporins are major determinants of water use efficiency of rice plants in the field

“…The water in the containers was aerated by brief bubbling and gas exchange measurements were taken again at 17:00. Root and leaf hydraulic conductivity was measured as described by Katsuhara et al [52].…”

Role of reactive oxygen species-generating enzymes and hydrogen peroxide during cadmium, mercury and osmotic stresses in barley root tip

“…Primers were prepared according to sequences published by Katsuhara et al (2003) for PIP2-1, Kristensen et al (1999) for PRX8, Lane et al (1993) for OxO, Lightfoot et al (2008) for NOXB1,B2,E,F1 and F2, Schultheiss et al (2003) for OLP with SOD activity, Suprunova et al (2004) for DHN1 and DHN6, Trujillo et al (2006) for NOXA and ubiquitin, and Wei et al (2007) for PIP1-2,3,4,5 and 6. Cycle numbers were optimised to assure that the amplification reaction was tested in the exponential phase and the gene product was detectable also in control roots.…”

“…It is well known that mercury (Hg) caused a large and rapid reduction of hydraulic conductivity of roots as consequence of AQP inhibition (Tazawa et al 1997). In barley, HvPIP2;1 protein was identified as the main AQP involved in the water transport of roots (Katsuhara et al 2003). From barley PIP1 family, PIP1-6 is expressed particularly in the growing compared with the non-growing tissues (Wei et al 2007).…”

Alterations of the gene expression, lipid peroxidation, proline and thiol content along the barley root exposed to cadmium

“…Primers were prepared according to sequences published by Choi et al (2002) for DHN4, by Finkemeier et al (2003) for PCS and MT; by Katsuhara et al (2003) for PIP; by Kristensen et al (1999) for PRX7, PRX8 and gylceraldehyde-3-phosphate dehydrogenase (GAPDH); by Metwally et al (2003) for APX, CAT, DHAR, GPX and GR; by Ouelhadj et al (2003) for HvC2; and by Suprunova et al (2004) for DHN1 and DHN6. Cycle numbers were optimised to assure that the amplification reaction was tested in the exponential phase and the gene product was also detectable in control roots.…”

“…Apart from constitutively expressed PIPs, several isoforms have been ascribed the function of adjusting plant water status during stress conditions (Luu and Maurel, 2005). In barley, for example, the HvPIP2;1 protein was identified as the main PIP involved in the mechanism of water transport in roots (Katsuhara et al, 2003).…”