Expression of an Animal Virus Antigenic Site on the Surface of a Plant Virus Particle

Usha, R.; Rohll, Jonathan B.; Spall, V. E.; Shanks, Michael; Maule, Andrew J.; Johnson, John E.; Lomonossoff, George P.

doi:10.1006/viro.1993.1598

Cited by 162 publications

(87 citation statements)

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“…Although live attenuated bacterial (Everest et al, 1995) and viral (Resnick et al, 1995;Rose & Evans, 1991;Sutter & Moss, 1992) vaccines as well as naked DNA vaccines (Montgomery et al, 1994) induce stronger and longer-lasting immune responses than conventional killed/subunit vaccines, even in the absence of adjuvant, there are safety concerns over the use of these vaccines (Vlacha et al, 1996;Klinman et al, 1997;WHO, 1990). …”

Section: Introductionmentioning

confidence: 99%

“…In an effort to produce effective non-replicating vaccines, we have been examining the vaccine potential of a plant virus, cowpea mosaic virus (CPMV), genetically engineered (Usha et al, 1993 ;Porta et al, 1994) to express and display foreign peptides on its surface. In this presentation system, termed EPICOAT, peptides of up ~zl., 199.5, 1996;Buratti et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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A chimaeric plant virus vaccine protects mice against a bacterial infection

Brennan¹,

Gilleland

Staczek

et al. 1999

Microbiology

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The plant virus cowpea mosaic virus (CPMV) is an efficient carrier of foreign peptides for the generation of strong humoral immune responses. Peptides derived from both viruses and bacteria are strongly immunogenic when displayed on the surface of CPMV and elicit high titres of peptide-specific antibody. However, the protective effects of antibodies generated using bacterial epitopes in this system have yet t o be demonstrated. In this study the ability of chimaeric virus particles (CVPs) t o afford protection against bacterial infection was assessed. Immunization of outbred mice with CPMV expressing a peptide derived from outer-membrane protein F of Pseudomonas aeruginosa (CPMV-PAE5) generated high titres of P. aeruginosa-specif ic IgG that opsonized the bacteria for phagocytosis by human neutrophils and afforded protection upon challenge with t w o different immunotypes of P. aeruginosa in a model of chronic pulmonary infection. When examined 8 d after challenge, CVP-immunized mice had fewer severe lung lesions and fewer bacteria in their lungs compared t o mice immunized with wild-type virus. Different levels of protection were seen with CPMV-PAE5 when Freund's or alum adjuvants were used. These studies highlight the ability of CVPs t o generate protective immunity against infectious disease agents.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A chimaeric plant virus vaccine protects mice against a bacterial infection

Brennan¹,

Gilleland

Staczek

et al. 1999

Microbiology

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“…Different species of viruses belonging to several families and genera (1)(2)(3)(4)(5)(6)(7)(8)(9) have been used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Immune response induced in mice oral immunization with cowpea severe mosaic virus

Florindo

Aragão

Silva

et al. 2002

Braz J Med Biol Res

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There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.

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“…The use of virus coat proteins of bacteria [1 3], animals [4][5][6][7] and plants [8][9][10] as carrier proteins has been reported. Recently, it has been shown that cowpea mosaic virus (CPMV) in which epitopes from animal viruses were inserted into the small coat protein, can present the epitopes on the surface of the virus particle, and the inserted epitopes are immunogenic in rabbits [10].…”

Section: Introductionmentioning

confidence: 99%