Expression of an ABA-responsive osmotin-like gene during the induction of freezing tolerance in Solanum commersonii

Zhu, Baolong; Chen, Tony H. H.; Li, Paul H.

doi:10.1007/bf00014558

Cited by 76 publications

(50 citation statements)

References 30 publications

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“…Many PR-type proteins are JA-and/or ET-inducible (150), and their occurrence can be further modulated by abscisic acid (7,89,113,181). Whereas in Arabidopsis a distinction between the SA-inducible PR-1, -2, and -5, and the JA/ET-inducible PR-3, -4, and -12 seems clear (143), in tobacco it has been demonstrated that different members within the same protein family are differentially regulated by SA and JA/ET (97,128).…”

Section: Signaling In the Induction Of Defense-related Proteinsmentioning

confidence: 99%

Significance of Inducible Defense-related Proteins in Infected Plants

Loon

Rep

Pieterse

2006

Annu. Rev. Phytopathol.

2,745

1,968

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Inducible defense-related proteins have been described in many plant species upon infection with oomycetes, fungi, bacteria, or viruses, or insect attack. Several types of proteins are common and have been classified into 17 families of pathogenesis-related proteins (PRs). Others have so far been found to occur more specifically in some plant species. Most PRs and related proteins are induced through the action of the signaling compounds salicylic acid, jasmonic acid, or ethylene, and possess antimicrobial activities in vitro through hydrolytic activities on cell walls, contact toxicity, and perhaps an involvement in defense signaling. However, when expressed in transgenic plants, they reduce only a limited number of diseases, depending on the nature of the protein, plant species, and pathogen involved. As exemplified by the PR-1 proteins in Arabidopsis and rice, many homologous proteins belonging to the same family are regulated developmentally and may serve different functions in specific organs or tissues. Several defense-related proteins are induced during senescence, wounding or cold stress, and some possess antifreeze activity. Many defense-related proteins are present constitutively in floral tissues and a substantial number of PR-like proteins in pollen, fruits, and vegetables can provoke allergy in humans. The evolutionary conservation of similar defense-related proteins in monocots and dicots, but also their divergent occurrence in other conditions, suggest that these proteins serve essential functions in plant life, whether in defense or not.

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Section: Signaling In the Induction Of Defense-related Proteinsmentioning

confidence: 99%

Significance of Inducible Defense-related Proteins in Infected Plants

Loon

Rep

Pieterse

2006

Annu. Rev. Phytopathol.

2,745

1,968

View full text Add to dashboard Cite

show abstract

“…However, the activity of GST-ABI2 was maxithe ABI2 mRNA appeared to be more weakly expressed in leaves. Figure 6B shows that the ABU and ABI2 mRNAs were induced by sorbitol, an experimental treatment that mimicks conditions of low water potential (Zhu et al, 1993). The abundance of both transcripts was reduced in the ABAdeficient abal mutant ( Figure 6B), suggesting that endogenous ABA upregulates the expression of these mRNAs.…”

Section: Comparative Analysis Of Abil-1 and Abi2-1 Mutantsmentioning

confidence: 98%

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

1997

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Abscisic acid (ABA) mediates seed maturation and adaptive responses t o environmental stress. In Arabidopsis, the ABA-INSENSITIVEl (ABll) protein phosphatase 2C is required for proper ABA responsiveness both in seeds and in vegetative tissues. To determine whether the lack of recessive alleles at the corresponding locus could be explained by the existence of redundant genes, we initiated a search for ABl7 homologs. One such homolog turned out t o be the A612 locus, whose abi2-7 mutation was previously known t o decrease ABA sensitivity. Whereas abi7-7 is (semi)dominant, abi2-7 has been described as recessive and maternally controlled at the germination stage. Unexpectedly, the sequence of the abi2-7 mutation showed that it converts Gly-168 t o Asp, which is precisely the same amino acid substitution found in abi7-7 and at the coincidental position within the A B l l phosphatase domain (Gly-180 t o Asp). In vitro assays and functional complementation studies in yeast confirmed that the AB12 protein is an active protein phosphatase 2C and that the abi2-7 mutation reduced phosphatase activity as well as affinity t o Mg2+. Although a number of differences between the two mutants in adaptive responses t o stress have been reported, quantitative comparisons of other major phenotypes showed that the effects of both abi7-7 and abi2-7 on these processes are nearly indistinguishable. Thus, the homologous ABll and AB12 phosphatases appear t o assume partially redundant functions in ABA signaling, which may provide a mechanism t o maintain informational homeostasis.

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“…Among stress proteins are osmotin and osmotin-like proteins that have also been classified as members of plant PR type-5 proteins (Singh et al, 1989;BOI et al, 1990;Zhu et al, 1993Zhu et al, , 1995. It has been demonstrated that tobacco osmotin gene expression is activated by ABA, NaC1, wounding, vira1 infection, and ethylene (LaRosa et al, 1992;Nelson et al, 1992).…”

mentioning

confidence: 99%

Activation of Two Osmotin-Like Protein Genes by Abiotic Stimuli and Fungal Pathogen in Transgenic Potato Plants

1995

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~Osmotin-like proteins are encoded by at least six members of a multigene family in Solanum commersonii. A genomic clone (ApCEM2a-7) that contains two osmotin-like protein genes (OSML13 and OSML81) arranged in the same transcriptional orientation has been isolated. Restriction mapping and sequence analysis indicated that the two intronless genes correspond to the previously characterized pA13 and pA81 cDNAs. To study the transcriptional activation of OSMLl3 and OSML8l promoters, the 5' flanking DNA sequence (-1078 to +35 of OSML13 and -1054 to +41 of OSML81) was fused to the p-glucuronidase (CUS) coding region, and the chimeric gene fusions were introduced into wild potato (S. commersonii) plants via Agrobacferiummediated transformation. Analysis of the chimeric gene expression in transgenic potato plants showed that both 5' flanking DNA sequences are sufficient to impart CUS inducibility by abscisic acid, NaCI, salicylic acid, wounding, and fungal infection. Low temperature activated both chimeric genes only slightly. lnfection with Phyfophthora infestam resulted in strong CUS expression from both chimeric genes primarily i n the sites of pathogen invasion, suggesting a limited diffusion of fungal infection-mediated signals. The expression patterns of both osmotin-like protein genes implicate their dual functions in osmotic stress and plant pathogen defense.

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Expression of an ABA-responsive osmotin-like gene during the induction of freezing tolerance in Solanum commersonii

Cited by 76 publications

References 30 publications

Significance of Inducible Defense-related Proteins in Infected Plants

Significance of Inducible Defense-related Proteins in Infected Plants

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

Activation of Two Osmotin-Like Protein Genes by Abiotic Stimuli and Fungal Pathogen in Transgenic Potato Plants

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