Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii

Li, Jun; Chen, Jinhui; Zhang, Yang; Yu, Ziniu

doi:10.1016/j.fsi.2012.11.012

Cited by 37 publications

(18 citation statements)

References 28 publications

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“…The allograft factor 1 (AIF-1) protein, a novel cytokine-like molecule involved in immunocyte recruitment into injured/grafted or infected tissues both in vertebrates [6, 7, 43] and invertebrates [8-10, 44], has recently been characterized in leech by our research group. Indeed, a gene showing high similarity with vertebrates’ AIF-1, named Hm Iba1/alias Hm AIF-1, has been recently characterized in the central nervous system of the medicinal leech [11].…”

Section: Discussionmentioning

confidence: 99%

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

et al. 2018

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Recent studies demonstrated that allograft inflammatory factor-1 (AIF-1) and RNASET2 act as chemoattractants for macrophages and modulate the inflammatory processes in both vertebrates and invertebrates. The expression of these proteins significantly increases after bacterial infection; however, the mechanisms by which they regulate the innate immune response are still poorly defined. Here, we evaluate the effect of bacterial lipopolysaccharide injection on the expression pattern of these genes and the interrelation between them during innate immune response in the medicinal leech, an invertebrate model with a simple anatomy and a marked similarity with vertebrates in inflammatory processes. Collectively, prokaryotic-eukaryotic co-cultures and in vivo infection assays suggest that RNASET2 and AIF-1 play a crucial role in orchestrating a functional cross-talk between granulocytes and macrophages in leeches, resulting in the activation of an effective response against pathogen infection. RNASET2, firstly released by granulocytes, likely plays an early antibacterial role. Subsequently, AIF-1⁺ RNASET2-recruited macrophages further recruit other macrophages to potentiate the antibacterial inflammatory response. These experimental data are in keeping with the notion of RNASET2 acting as an alarmin-like molecule whose role is to locally transmit a “danger” signal (such as a bacterial infection) to the innate immune system in order to trigger an appropriate host response.

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Section: Discussionmentioning

confidence: 99%

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

et al. 2018

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“…This molecule was shown to be induced in coelomocytes exposed to bacterial challenge (Ovando et al, ). Invertebrate AIF‐1–like molecules were mainly described in Mollusks, notably in Gastropods (De Zoysa et al, ) and Bivalves (Zhang et al, ; Li et al, ). In these animals, the respective AIF‐1 genes are expressed in a variety of unstimulated tissues, suggesting a role not necessarily linked to allogenic or immune inflammation and are upregulated in different organs upon bacterial challenge.…”

Section: Discussionmentioning

confidence: 99%

“…Although these proteins share a highly conserved amino acid sequence, their functional identity remains to be elucidated (Deininger et al, ). Recent reports showed that proteins of the AIF‐1 superfamily, well distributed in Vertebrates, are also present in a few other groups of animals, including Sponges (Kruse et al, ), Mollusks (De Zoysa et al, ; Zhang et al, ; Li et al, ) and Echinoderms (Ovando et al, ). The comparison of such sequences shows a good similarity of protein primary structures, suggesting a significant conservation of functional properties of AIF‐1 factors among Metazoa.…”

Section: Introductionmentioning

confidence: 99%

Microglia of medicinal leech (Hirudo medicinalis) express a specific activation marker homologous to vertebrate ionized calcium‐binding adapter molecule 1 (Iba1/alias aif‐1)

Drago¹,

Sautière²,

Marrec-Croq³

et al. 2014

Developmental Neurobiology

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The Ionized calcium-Binding Adapter molecule 1 (Iba1), also known as Allograft Inflammatory Factor 1 (AIF-1), is a 17 kDa cytokine-inducible protein, produced by activated macrophages during chronic transplant rejection and inflammatory reactions in Vertebrates. In mammalian central nervous system (CNS), Iba1 is a sensitive marker associated with activated macrophages/microglia and is upregulated following neuronal death or brain lesions. The medicinal leech Hirudo medicinalis is able to regenerate its CNS after injury, leading to a complete functional repair. Similar to Vertebrates, leech neuroinflammatory processes are linked to microglia activation and recruitment at the lesion site. We identified a gene, named Hmiba1, coding a 17.8 kDa protein showing high similarity with Vertebrate AIF-1. The present work constitutes the first report on an Iba1 protein in the nervous system of an invertebrate. Immunochemistry and gene expression analyses showed that HmIba1, like its mammalian counterpart, is modulated in leech CNS by mechanical injury or chemical stimuli (ATP). We presently demonstrate that most of leech microglial cells migrating and accumulating at the lesion site specifically expressed the activation marker HmIba1. While the functional role of Iba1, whatever species, is still unclear in reactive microglia, this molecule appeared as a good selective marker of activated cells in leech and presents an interesting tool to investigate the functions of these cells during nerve repair events.

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“…In contrast, Hsp70 gene expression of the coral Desmophyllum dianthus to elevated pCO 2 (997 μatm) for 8 months was strongly upregulated, along with the gene expression of mannosebinding C-type lectin [48]. Allograft inflammatory factor 1-like, another S. glomerata DE transcript that was found to be 2-fold and higher up-regulated in CO 2 stressed oysters (Additional file 7: Table S2) has been shown to be induced by bacterial challenge, tissue injury and shell damage in the pearl oyster Pinctada martensii, suggesting a role in innate immunity [49]. These results indicate that stressors such as pCO 2 and tissue injury appear to result in a similar induction of the affected animal's immune system, as has been seen in the S. glomerata exposed to elevated pCO 2 of this study.…”

Section: Immunitymentioning

confidence: 99%

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

Ertl

O’Connor

Wiegand

et al. 2016

Clim Chang Responses

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Background: Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO 2 . Results: A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated pCO 2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO 2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO 2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions: This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO 2 .

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Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii

Cited by 37 publications

References 28 publications

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

AIF-1 and RNASET2 Play Complementary Roles in the Innate Immune Response of Medicinal Leech

Microglia of medicinal leech (Hirudo medicinalis) express a specific activation marker homologous to vertebrate ionized calcium‐binding adapter molecule 1 (Iba1/alias aif‐1)

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

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