Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissue

Campbell, Wendy J.; Larsen, Douglas; Deb, S.; Kwok, Simon C. M.; Soares, Michael J.

doi:10.1016/0143-4004(91)90004-y

Cited by 17 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with results obtained by Northern blot analysis of rat placenta on days 16 and 19 or gestation (Campbell et al 1991).…”

Section: Discussionsupporting

confidence: 82%

“…However, neither of these methods distinguishes between isoforms of the enzyme. For this purpose Campbell et al (1991) employed Northern blot analysis and investigated extracts of rat placental lab\-rinth and junctional zones for the presence of TNAP mRNA. However, when attempting to locate specific messenger RNAs in a composite tissue, it is important to complement Northern hybridization with in situ hybridization histochemistry.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry

et al. 1993

View full text Add to dashboard Cite

Alkaline phosphatases (APs) are a family of cell surface glycoproteins that are expressed in a variety of tissues. Their physiological functions are still unclear. Three different AP genes have been found to be expressed in mice, and AP cloned from the placenta is of the tissue non-specific (TNAP) type. We have in investigated the location of TNAP mRNA and active AP in mature mouse placenta, using in situ hybridization and enzyme histochemistry on serial sections. Digital image analysis was used to estimate relative amounts of TNAP mRNA. Tissue non-specific alkaline phosphatase messenger was detected only in the placental labyrinth, whereas active AP was present both in the labyrinth and in a zone of cells at the margin of the decidua basalis, bordering the myometrium and the metrial gland. This latter location of AP activity has not been described previously. The AP-positive zone of the decidua had a condensed appearance and a central defect in the zone was visible on sections taken from the middle of the placenta. No TNAP messenger was found in the zone of AP-positive decidual cells.

show abstract

“…This is in agreement with results obtained by Northern blot analysis of rat placenta on days 16 and 19 or gestation (Campbell et al 1991).…”

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry

et al. 1993

View full text Add to dashboard Cite

show abstract

“…In addition, cell fusion and syncytial formation in the placenta have been associated with the increased expression of cadherin-11 and protein modification of placental alkaline phosphatase (40)(41)(42)(43). Thus, we investigated these changes in TGF-␤-treated SM10 and HRP-1 cell lines.…”

Section: Expression Of Labyrinthine Trophoblast Fusion Markers Gcm1 mentioning

confidence: 94%

“…The PVDF membrane was blocked in 5% (wt/vol) nonfat milk, pH 7.4, in 1ϫ PBS containing 0.05% Tween-20 (PBST) for 30 min and probed with appropriate primary antibodies. Rabbit polyclonal anti-placental alkaline phosphatase antibody, which recognizes the unmodified and modified placental alkaline phosphatases, was used at 1/2,000 overnight at 4°C (76), and mouse anti-cadherin-11 antibody at 1/1,000 overnight at 4°C in blocking buffer. The membrane was washed with PBST, probed with HRP-conjugated, appropriate secondary antibody at 1/10,000 for 1 h at room temperature, and detected using Enhanced Chemi-Luminiscence reagent according to manufacturer's instructions.…”

Section: Western Blottingmentioning

confidence: 99%

“…Fused labyrinthine trophoblasts have also been shown to exhibit a uniquely glycosylated placental alkaline phosphatase protein (PLAP) (43). Even though the function of PLAP is not understood, its localization to syncytial trophoblasts has been useful for assessing the purity of placental preparations and as a marker of syncytial trophoblast cells (43)(44)(45).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transforming Growth Factor-βInduces Differentiation of the Labyrinthine Trophoblast Stem Cell Line SM10

Selesniemi

Reedy

Gultice

et al. 2005

Stem Cells and Development

View full text Add to dashboard Cite

The mammalian placenta consists of different trophoblast cell types that assist in the variety of functions required for the maintenance of pregnancy. In rodents, labyrinthine trophoblasts of the placenta are especially important, because they are capable of differentiating into fused labyrinthine cells, which form the feto-maternal exchange surface. Even though the molecular signals triggering labyrinthine trophoblast differentiation are poorly understood, transforming growth factor-beta (TGF-beta) has been shown to be present in the placental environment and alter trophoblast development. In this study, we investigated the effects of TGF-beta on the differentiation of the labyrinthine trophoblast stem cell lines SM10 and HRP-1. RT-PCR analyses demonstrated that while the molecular expression of labyrinthine-specific lineage markers (Esx1, Tfeb, and Tec) was maintained in TGF-beta-treated SM10 and HRP-1 cells, TGF-beta induced the down-regulation of trophoblast stem cell markers Id2 and Cdx2. In contrast, TGF-beta induced the expression of a marker of differentiated labyrinthine trophoblasts, Gcm1, only in the SM10 cell line. Furthermore, we demonstrated an increased glucose uptake in the TGF-beta-treated SM10 cells, indicative of functional differentiation. Finally, cell fusion in TGF-beta-treated SM10 and HRP-1 cells was investigated by western blotting analysis of placental alkaline phosphatase and cadherin-11 and by microscopic analyses of cell morphology using green fluorescent protein (GFP) and rhodamine phalloidin staining. The western blotting and morphological analyses indicate TGF-beta-induced cell fusion and morphological differentiation in the SM10 cell line. The SM10 cell line will provide a new and unique model for detailed analysis of TGF-beta-induced molecular events associated with labyrinthine trophoblast differentiation and function.

show abstract

The human immunodeficiency virus(HIV) inhibitor 9‐(2‐phosphonylmethoxyethyl)adenine (pmea) is a strong inducer of differentiation of several tumor cell lines

Balzarini

Verstuyf

Hatse

et al. 1995

Intl Journal of Cancer

View full text Add to dashboard Cite

9-(2-phosphonylmethoxyethyl)adenine (PMEA) is the prototype compound of a series of acyclic nucleoside phosphonate derivatives endowed with potent and selective anti-retroviral activity in vitro and in vivo. We have now found that PMEA is also a potent inducer of differentiation of a number of tumor cells, including human erythroleukemia K562 cells, rat choriocarcinoma RCHO cells and human acute promyelocytic leukemic HL-60 cells. At 10 microM PMEA, rat RCHO cell cultures could be almost fully differentiated, and at 50 microM PMEA, approximately 50% of the K562 cells could be triggered to produce hemoglobin. The differentiating activity of butyric acid was at least partially additive to that of PMEA when both drugs were combined in K562 cell cultures. PMEA needs to be present for at least 2 or 3 days in the K562 cell cultures to achieve optimal differentiating activity. This suggests that either a PMEA metabolite and/or its anti-metabolic effects may be responsible for differentiation of the tumor cells.

show abstract

Expression of alkaline phosphatase in differentiated rat labyrinthine trophoblast tissue

Cited by 17 publications

References 23 publications

Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry

Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistry

Transforming Growth Factor-βInduces Differentiation of the Labyrinthine Trophoblast Stem Cell Line SM10

The human immunodeficiency virus(HIV) inhibitor 9‐(2‐phosphonylmethoxyethyl)adenine (pmea) is a strong inducer of differentiation of several tumor cell lines

Contact Info

Product

Resources

About