Expression of agsA, one of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress

Damveld, Robbert A.; vanKuyk, Patricia A.; Arentshorst, Mark; Klis, Frans M.; Hondel, Cees A. M. J. J. van den; Ram, Arthur F. J.

doi:10.1016/j.fgb.2004.11.006

Cited by 81 publications

(87 citation statements)

References 72 publications

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“…Analysis of its sequence suggests a resemblance to previously reported fungal AGS [24,26]. As with other a-glucan synthases, three distinct domains can be identified in the PbrAgs1 sequence.…”

Section: Paracoccidioides Brasiliensis and Its Cell Wallsupporting

confidence: 63%

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Paracoccidioides brasiliensis: chemical and molecular tools for research on cell walls, antifungals, diagnosis, taxonomy

San-Blas

Niño-Vega

2008

Mycopathologia

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Paracoccidioides brasiliensis is a dimorphic fungus, a causative agent of paracoccidioidomycosis, one of the most frequent systemic mycoses that affect the rural population in Latin America, only geographical region in which this fungus is to be found. In this work, we discuss matters related to (a) cell wall studies based on the cloning and analysis of genes involved in the synthesis of cell wall components, and their possible roles in virulence and dimorphism in P. brasiliensis, (b) molecular taxonomy and the molecular classification of P. brasiliensis as an Ascomycete belonging in the Order Onygenales, (c) phylogeny of P. brasiliensis and the possible existence of cryptic species within the genus Paracoccidioides, and (d) new experimental antifungal drugs such as azasterols or sterol hydrazones, compounds that affect the activity of D 24(28) sterol methyl reductase (SMR) and/or D (24) -sterol methyl transferase (SMT), and (e) specific primers for the molecular detection of P. brasiliensis in vitro and in clinical samples.

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Section: Paracoccidioides Brasiliensis and Its Cell Wallsupporting

confidence: 63%

“…Damvel et al [26] identified a family of five a-1,3-glucan synthase-encoding genes in A. niger and three in Penicillium chrysogenum. Once sequenced, the predicted protein sequence of the agsA gene indicated that the overall domain structure of a-1,3-glucan synthases is conserved in fungi.…”

Section: Paracoccidioides Brasiliensis and Its Cell Wallmentioning

confidence: 99%

Paracoccidioides brasiliensis: chemical and molecular tools for research on cell walls, antifungals, diagnosis, taxonomy

San-Blas

Niño-Vega

2008

Mycopathologia

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“…The screen is based on the observation that the agsA gene, which encodes a putative a-1,3-glucan synthase, is strongly induced in response to different forms of cell wall stress (Damveld et al 2005a;Meyer et al 2007b). Important factors for such a successful genetic screen are:…”

Section: Discussionmentioning

confidence: 99%

“…A. niger contains a family of five ags genes. Two of them, agsA and to a lesser extent agsE, are induced in response to calcofluor white (CFW)-induced cell wall stress (Damveld et al 2005a). Induced expression of agsA is dependent on the RlmA transcription factor, a homolog of the Rlm1p transcription factor in S. cerevisiae (Damveld et al 2005b).…”

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confidence: 99%

“…Construction of recombinant plasmids: For the construction of plasmid PagsA-amdS-TamdS, a 2010-bp SalI-EcoRI fragment containing the agsA promoter region was isolated from pRD12 (Damveld et al 2005a) and ligated into SalI-EcoRI digested pBluescipt II SK (Stratagene, La Jolla, CA). The resulting plasmid was linearized with EcoRI and XbaI yielding the first fragment for a three-way ligation.…”

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A Novel Screening Method for Cell Wall Mutants in Aspergillus niger Identifies UDP-Galactopyranose Mutase as an Important Protein in Fungal Cell Wall Biosynthesis

et al. 2008

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To identify cell wall biosynthetic genes in filamentous fungi and thus potential targets for the discovery of new antifungals, we developed a novel screening method for cell wall mutants. It is based on our earlier observation that the Aspergillus niger agsA gene, which encodes a putative a-glucan synthase, is strongly induced in response to cell wall stress. By placing the agsA promoter region in front of a selectable marker, the acetamidase (amdS) gene of A. nidulans, we reasoned that cell wall mutants with a constitutively active cell wall stress response pathway could be identified by selecting mutants for growth on acetamide as the sole nitrogen source. For the genetic screen, a strain was constructed that contained two reporter genes controlled by the same promoter: the metabolic reporter gene PagsA-amdS and PagsA-H2B-GFP, which encodes a GFP-tagged nuclear protein. The primary screen yielded 161 mutants that were subjected to various cell wall-related secondary screens. Four calcofluor white-hypersensitive, osmotic-remediable thermosensitive mutants were selected for complementation analysis. Three mutants were complemented by the same gene, which encoded a protein with high sequence identity with eukaryotic UDP-galactopyranose mutases (UgmA). Our results indicate that galactofuranose formation is important for fungal cell wall biosynthesis and represents an attractive target for the development of antifungals.

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Microarray hybridization analysis of light-dependent gene expression inPenicillium chrysogenumidentifies bZIP transcription factor PcAtfA

Wolfers

Kamerewerd

Nowrousian

et al. 2015

J. Basic Microbiol.

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The fungal velvet complex is a light-dependent master regulator of secondary metabolism and development in the major penicillin producer, Penicillium chrysogenum. However, the light-dependent mechanism is unclear. To identify velvet-dependent transcriptional regulators that show light-regulated expression, we performed microarray hybridizations with RNA isolated from P. chrysogenum ΔPcku70 cultures grown under 13 different long-term, light-dependent growth conditions. We compared these expression data to data from two velvet complex deletion mutants; one lacked a subunit of the velvet complex (ΔPcvelA), and the other lacked a velvet-associated protein (ΔPclaeA). We sought to identify genes that were up-regulated in light, but down-regulated in ΔPcvelA and ΔPclaeA. We identified 148 co-regulated genes that displayed this regulatory pattern. In silico analyses of the co-regulated genes identified six proteins with fungal-specific transcription factor domains. Among these, we selected the bZIP transcription factor, PcAtfA, for functional characterization in deletion and complementation strains. Our data clearly indicates that PcAtfA governs spore germination. This comparative analysis of different microarray hybridization data sets provided results that may be useful for identifying genes for future functional analyses.

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Expression of agsA, one of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress

Cited by 81 publications

References 72 publications

Paracoccidioides brasiliensis: chemical and molecular tools for research on cell walls, antifungals, diagnosis, taxonomy

Paracoccidioides brasiliensis: chemical and molecular tools for research on cell walls, antifungals, diagnosis, taxonomy

A Novel Screening Method for Cell Wall Mutants in Aspergillus niger Identifies UDP-Galactopyranose Mutase as an Important Protein in Fungal Cell Wall Biosynthesis

Microarray hybridization analysis of light-dependent gene expression inPenicillium chrysogenumidentifies bZIP transcription factor PcAtfA

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