Expression of Aeromonas caviae ST pyruvate dehydrogenase complex components mediate tellurite resistance in Escherichia coli

Castro, Miguel E.; Molina, Roberto C.; Díaz, Waldo A.; Pradenas, Gonzalo A.; Vásquez, Claudio C.

doi:10.1016/j.bbrc.2009.01.078

Cited by 28 publications

(23 citation statements)

References 27 publications

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“…Antibiotics acting by interfering with cell wall (ampicillin, cefotaxime) or protein synthesis (tetracycline, chloramphenicol, gentamicin), when used with sub-lethal concentrations of tellurite, their killing action is potentiated many folds. The possible mechanisms other than Reactive oxygen species generation [34], are damage to metabolic enzymes [35,36], glutathione depletion [37] or lipid peroxidation [38].…”

Section: Antimicrobial Adjuvantsmentioning

confidence: 99%

Novel Strategies to Combat Antimicrobial Resistance

Kaur¹

2016

J Infect Dis Ther

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Infectious diseases form the major health-care burden for the developing world and antimicrobials prove to be the magical drugs to combat this. The discovery of antimicrobial agents was boon for the global health-care system and the wonderful cure by antimicrobials shifted the disease trends from infectious to life-style diseases in the developed world. Sudden appearance of the antimicrobial resistance hampered the whole success; and this situation is further complicated by the dry pipeline of antimicrobial development. Now, this is heading the world towards the "preantibiotic" era. The development of new antimicrobials is not able to match pace with the speedily growing antimicrobial resistance. Development of new active pharmaceutical principles is a difficult and costly practice. The other approach to achieve the same is by rejuvenating the existing antimicrobials. These contemporary novel approaches include bacteriophage therapy, fecal microbiota transplantation, antimicrobial peptides, combination drug therapy and antimicrobial adjuvants to combat antimicrobial resistance forms the main stay of discussion of this article.

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Section: Antimicrobial Adjuvantsmentioning

confidence: 99%

Novel Strategies to Combat Antimicrobial Resistance

Kaur¹

2016

J Infect Dis Ther

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“…More recently, we have shown that overproduction of the Aeromonas caviae ST dihydrolipoil dehydrogenase results in enhanced tellurite resistance in E. coli. This enzyme exhibits NADH-dependent tellurite reductase (TR) activity (Castro et al, 2008(Castro et al, , 2009). The change of two defined amino acid residues at the enzyme active site decreased TR activity (unpublished data).…”

Section: The Enigma Of Tellurite Toxicitymentioning

confidence: 99%

“…In addition to its high resistance to tellurite, this strain exhibited high levels of tellurite reduction, as determined by the darkness of cells exposed to the toxic salt and by tellurite reductase (TR) enzymatic assays performed with cell-free extracts. Interestingly, most of this TR activity was dependent of NADH and tracked to the pyruvate dehydrogenase multienzymatic complex (PDH), specifically to the E3 component encoded by the lpdA gene (Castro et al, 2008(Castro et al, , 2009). …”

Section: Lpdamentioning

confidence: 99%

“…These mutants were chosen based on previous work on the E. coli E3 component indicating that C45 is highly conserved and is involved in the formation of a disulfide bond with C50, required for appropriate protein conformation (Kim et al, 2008). H322 and E354 were changed to Y and K, respectively, because it was previously shown that these mutations affect NADH binding, a substrate required for PDH as well as for TR activity (Castro et al, 2008(Castro et al, , 2009). …”

Section: Lpdamentioning

confidence: 99%

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Site-Directed Mutagenesis as a Tool for Unveiling Mechanisms of Bacterial Tellurite Resistance

Pérez-Donoso¹,

Vásquez²

2013

Genetic Manipulation of DNA and Protein - Examples From Current Research

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“…Microorganisms are known to catalyze the reduction of toxic tellurite into sparingly soluble and less toxic elemental tellurium [Te(0)] (10)(11)(12)(13)(14)(15). In comparison, little is known about the microbial reduction of tellurate, even though Te(VI) is the dominant form of Te in the hydrosphere (16,17).…”

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Genetic Evidence for a Molybdopterin-Containing Tellurate Reductase

Theisen

Zylstra

Yee

2013

Appl Environ Microbiol

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The genetic identity and cofactor composition of the bacterial tellurate reductase are currently unknown. In this study, we examined the requirement of molybdopterin biosynthesis and molybdate transporter genes for tellurate reduction in Escherichia coli K-12. The results show that mutants deleted of the moaA, moaB, moaE, or mog gene in the molybdopterin biosynthesis pathway lost the ability to reduce tellurate. Deletion of the modB or modC gene in the molybdate transport pathway also resulted in complete loss of tellurate reduction activity. Genetic complementation by the wild-type sequences restored tellurate reduction activity in the mutant strains. These findings provide genetic evidence that tellurate reduction in E. coli involves a molybdoenzyme.T ellurium (Te) is a metalloid element used for a variety of industrial applications, including metallurgy, chemical manufacturing, electronics, and nanotechnology (1-4). The disposal of mine tailings and tellurium-containing waste has led to an increase in environmental contamination (5) . These oxyanions are highly toxic to microbiota (6) and cause inhibitory effects to most microorganisms at concentrations as low as 1 g/ml (1, 4). While naturally occurring Te-resistant bacteria have been isolated that are able to grow in the presence of elevated Te concentrations (7,8) and several genetic elements have been linked to Te resistance (9), the molecular mechanisms of bacterium-tellurium interactions remain poorly understood.Microorganisms are known to catalyze the reduction of toxic tellurite into sparingly soluble and less toxic elemental tellurium [Te(0)] (10-15). In comparison, little is known about the microbial reduction of tellurate, even though Te(VI) is the dominant form of Te in the hydrosphere (16,17). Recently, Shewanella species isolated from deep-ocean hydrothermal vent worms were discovered to respire Te(VI) as a terminal electron acceptor (18), and the anaerobic bacteria Sulfurospirillum barnesii and Bacillus selenitireducens were found to generate energy for growth on lactate by the reduction of Te(VI) to Te(0) (7). A Gram-positive bacterium, Bacillus beveridgei, was recently isolated that can also grow by reducing Te(VI) to Te(0) (8). The genetic identity and cofactor composition of enzymes that catalyze tellurate reduction in these bacteria, as well as other Te(VI) reducers, are currently unknown.The molybdenum cofactor forms the active site of several important bacterial redox proteins, including the enzymes that catalyze the reduction of selenium and arsenic (15,(19)(20)(21)(22)(23), two metalloid elements that share similar chemical characteristics with tellurium. In selenate [Se(VI)]-and arsenate [As(V)]-reducing bacteria, the assembly of the molybdoenzymes is dependent on molybdenum import into the cell and biosynthesis of the molybdopterin cofactor. Previous studies have demonstrated that molybdate uptake in Escherichia coli is facilitated by an ABC-type transporter, and the molybdopterin cofactor is constructed via a biosynthetic pathway enc...

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Expression of Aeromonas caviae ST pyruvate dehydrogenase complex components mediate tellurite resistance in Escherichia coli

Cited by 28 publications

References 27 publications

Novel Strategies to Combat Antimicrobial Resistance

Novel Strategies to Combat Antimicrobial Resistance

Site-Directed Mutagenesis as a Tool for Unveiling Mechanisms of Bacterial Tellurite Resistance

Genetic Evidence for a Molybdopterin-Containing Tellurate Reductase

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