Expression of adhesion molecules on endothelial cells after contact with knitted Dacron

Cenni, E.; Granchi, Donatella; Ciapetti, G.; Verri, E.; Cavedagna, D.; Gamberini, Simonetta; Cervellati, M.; Leo, Alessandro Di; Pizzoferrato, Alberto

doi:10.1016/s0142-9612(96)00160-3

Cited by 45 publications

(33 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, expression of these adhesion molecules was determined as a function of EC seeding density and culture time. This is in contrast with other studies [9][10][11][12] in which adhesion molecule expressions were measured only after confluence and at a single time point.…”

Section: Introductioncontrasting

confidence: 72%

See 1 more Smart Citation

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.

show abstract

Section: Introductioncontrasting

confidence: 72%

“…[9][10][11] In contrast, the MFI of ICAM-1 expression increases during proliferation, indicating a gradual and specific activation of ECs. The fact that only the ICAM-1 expression is affected could be an indication of the way cell activation is triggered.…”

Section: Discussionmentioning

confidence: 96%

ICAM‐1 and VCAM‐1 expression by endothelial cells grown on fibronectin‐coated TCPS and PS

Zijpp

Poot

Feijén

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…For example, different levels of ELAM are expressed on HUVEC cultured on knitted versus woven Dacron [151] and different neutrophil adhesion [152], tissue factor and prostacyclin levels have been observed on Dacron grafts versus smooth films [153]. The goal of many of the studies listed [154][155][156][157][158][159][160][161][162][163] was to compare particular vascular graft materials, with or without different ECM coatings, to TCPS, and thus differences can potentially be attributed to topography rather than surface chemistry.…”

Section: Molecular Expression Studiesmentioning

confidence: 99%

The influence of biomaterials on endothelial cell thrombogenicity

2007

View full text Add to dashboard Cite

Driven by tissue engineering and regenerative medicine, endothelial cells are being used in combination with biomaterials in a number of applications for the purpose of improving blood compatibility and host integration. Endothelialized vascular grafts are beginning to be used clinically with some success in some centers, while endothelial seeding is being explored as a means of creating a vasculature within engineered tissues. The underlying assumption of this strategy is that when cultured on artificial biomaterials, a confluent layer of endothelial cells maintain their nonthrombogenic phenotype. In this review the existing knowledge base of endothelial cell thrombogenicity cultured on a number of different biomaterials is summarized. The importance of selecting appropriate endpoint measures that are most reflective of overall surface thrombogenicity is the focus of this review. Endothelial cells inhibit thrombosis through three interconnected regulatory systems (1) the coagulation cascade (2) the cellular components of the blood such as leukocytes and platelets and (3) the complement cascade, and also through effects on fibrinolysis and vascular tone, the latter which influences blood flow. Thus, in order to demonstrate the thromobgenic benefit of seeding a biomaterial with EC, the conditions under which EC surfaces are more likely to exhibit lower thrombogenicity than unseeded biomaterial surfaces need to be consistent with the experimental context. The endpoints selected should be appropriate for the dominant thrombotic process that occurs under the given experimental conditions.

show abstract

“…8 -12 Since these studies, numerous laboratories have focused on the preparation of polymer surfaces to enhance EC attachment (Table 1). [13][14][15][16][17][18][19][20][21][22][23][24][25][26] This approach has been limited in some cases by poor retention of ECs on biomaterial surfaces once exposed to blood flow. Thus, significant effort has focused on improving endothelial adhesion to biomaterial surfaces.…”

Section: Endothelial Cell Seeding Of Vascular Graftsmentioning

confidence: 99%