Expression of adhesion molecules, monocyte interactions and oxidative stress in human endothelial cells exposed to wood smoke and diesel exhaust particulate matter

Forchhammer, Lykke; Loft, Steffen; Roursgaard, Martin; Cao, Yi; Riddervold, Ingunn Skogstad; Sigsgaard, Torben; Möller, Peter

doi:10.1016/j.toxlet.2011.12.003

Cited by 58 publications

(41 citation statements)

References 37 publications

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“…Six hours exposure of human lung epithelial cells to high concentrations of fullerene C 60 nanoparticles increased GSH levels (Wang et al, 2014). Our previous work showed that 3 h exposure to DEP or carbon-based particles induced ROS in a number of different types of human cells (Cao et al, 2014a,b;Forchhammer et al, 2012;Frikke-Schmidt et al, 2011;Jantzen et al, 2012;Vesterdal et al, 2012Vesterdal et al, , 2014. However, exposure of primary human endothelial cells to carbon black nanoparticles (Printex90) only significantly increased reduced GSH levels at 24 h, but did not significantly affect it or expression of antioxidant enzymes (glutamate-cysteine ligase modifier subunit and heme oxygenase 1) after 3 h (Cao et al, 2014b).…”

Section: Discussionmentioning

confidence: 93%

Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

Cao¹,

Jantzen²,

Gouveia³

et al. 2015

Environmental Toxicology and Pharmacology

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Section: Discussionmentioning

confidence: 93%

Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

Cao¹,

Jantzen²,

Gouveia³

et al. 2015

Environmental Toxicology and Pharmacology

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“…Toxicological effects related to human health Human controlled exposure studies report that short-term inhalation of woodsmoke induces mild inflammatory effects including distal airway inflammation, increased oxidative stress and immune cell recruitment, but no effects on measures of lung function [98][99][100][101][102][103]. Studies also report systemic effects such as increased levels of coagulation markers, and decreased heart rate variability and systemic inflammation [100,[104][105][106][107].…”

Section: Toxicity Of Woodsmoke Particlesmentioning

confidence: 99%

Health impacts of anthropogenic biomass burning in the developed world

Sigsgaard¹,

et al. 2015

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Climate change policies have stimulated a shift towards renewable energy sources such as biomass. The economic crisis of 2008 has also increased the practice of household biomass burning as it is often cheaper than using oil, gas or electricity for heating. As a result, household biomass combustion is becoming an important source of air pollutants in the European Union.This position paper discusses the contribution of biomass combustion to pollution levels in Europe, and the emerging evidence on the adverse health effects of biomass combustion products.Epidemiological studies in the developed world have documented associations between indoor and outdoor exposure to biomass combustion products and a range of adverse health effects. A conservative estimate of the current contribution of biomass smoke to premature mortality in Europe amounts to at least 40 000 deaths per year.We conclude that emissions from current biomass combustion products negatively affect respiratory and, possibly, cardiovascular health in Europe. Biomass combustion emissions, in contrast to emissions from most other sources of air pollution, are increasing. More needs to be done to further document the health effects of biomass combustion in Europe, and to reduce emissions of harmful biomass combustion products to protect public health. @ERSpublications Biomass combustion is an important source of air pollution and ill health in the EU: emissions need to reduce http://ow.ly/RYkPk

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“…In our previous work, we have incubated the probe-loaded cells with the particles and continuously measured the ROS for 3 h (Cao et al, 2014;Forchhammer et al, 2012;Vesterdal et al, 2012). However, preliminary experiments showed that the presence of BSA could interfere with the assay.…”

Section: Ros Measurementmentioning

confidence: 99%

“…The protein level of tumor necrosis factor (TNF), interleukin (IL)-6 and IL-8 was measured in the medium from the lower chamber to indicate inflammatory responses. The adhesion of THP-1 monocytes to HUVECs was used as a major endpoint since the recruitment of monocytes is an important characteristic in the pathogenesis of atherosclerosis (Moore & Tabas, 2011), and increased monocyte adhesion to HUVECs has been observed by in vitro exposure to, e.g., wood smoke particles (Forchhammer et al, 2012) as well as MWCNTs (Cao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Monocyte adhesion induced by multi-walled carbon nanotubes and palmitic acid in endothelial cells and alveolar–endothelial co-cultures

et al. 2015

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Free palmitic acid (PA) is a potential pro-atherogenic stimulus that may aggravate particle-mediated cardiovascular health effects. We hypothesized that the presence of PA can aggravate oxidative stress and endothelial activation induced by multi-walled carbon nanotube (MWCNT) exposure in vitro. We investigated the interaction between direct exposure to MWCNTs and PA on THP-1 monocyte adhesion to human umbilical vein endothelial cells (HUVECs), as well as on indirect exposure in an alveolar-endothelial co-culture model with A549 cells and THP-1-derived macrophages exposed in inserts and the effect measured in the lower chamber on HUVECs and THP-1 cells. The exposure to MWCNTs, including a short (NM400) and long (NM402) type of entangled fibers, was associated with elevated levels of reactive oxygen species as well as a decrease in the intracellular glutathione concentration in HUVEC and A549 monocultures. Both effects were found to be independent of the presence of PA. MWCNT exposure significantly increased THP-1 monocyte adhesion to HUVECs, and co-exposure to PA aggravated the NM400-mediated adhesion but decreased the NM402-mediated adhesion. For the co-cultures, the exposure of A549 cells did not promote THP-1 adhesion to HUVECs in the lower chamber. When THP-1 macrophages were present on the cell culture inserts, there was a modest increase in the adhesion and an increase in interleukin-6 and interleukin-8 levels in the lower chamber whereas no tumor necrosis factor was detected. Overall, this study showed that direct exposure of HUVECs to MWCNTs was associated with oxidative stress and monocyte adhesion and the presence of PA increased the adhesion when exposed to NM400.

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Expression of adhesion molecules, monocyte interactions and oxidative stress in human endothelial cells exposed to wood smoke and diesel exhaust particulate matter

Cited by 58 publications

References 37 publications

Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

Automobile diesel exhaust particles induce lipid droplet formation in macrophages in vitro

Health impacts of anthropogenic biomass burning in the developed world

Monocyte adhesion induced by multi-walled carbon nanotubes and palmitic acid in endothelial cells and alveolar–endothelial co-cultures

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