Expression of Adhesion Molecules in Nonallergic Chronic Sinusitis

Shimomura, Akira; Ikeda, Katsuhisa; Suzuki, Hideaki; Nakabayashi, Seiichiro; Oshima, Takeshi; Furukawa, Masayuki; Takasaka, Tomonori; Ando, Noriaki; Ohtani, Haruo; Nishimura, Hiroshi

doi:10.1097/00005537-199711000-00018

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…Administration of EM inhibited accumulation of neutrophils in the middle ear after LPS stimulation. It is well known that adhesion molecules are involved in the infiltration of leukocytes to the foci of inflammation, and that the modulation of adhesion molecules affects the migration of leukocytes (6,7). Leukocytes migrate into the inflammatory tissues through the vascular endothelia in two stages: the first stage is called rolling, where L-selectin is involved, and the second stage is called firm adhesion, where the i 2 -integrins Mac-1 and LFA-1 are involved.…”

Section: Discussionmentioning

confidence: 99%

Effect of Erythromycin on Otitis Media with Effusion in Experimental Rat Model

Enomoto¹,

Ichikawa²,

Nagaoka³

et al. 1998

Acta Oto-Laryngologica

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Enomoto F, Ichikawa G, Nagaoka I, Yamashita T. Effect of erythromycin on otitis media with effusion in experimental rat model. Acta Otolaryngol (Stockh) 1998; Suppl 539: 57-60.To understand the mechanism of erythromycin (EM) on otitis media with effusion, we examined its effects on leukocyte accumulation and expression of adhesion molecules L-selectin and Mac-1, using a rat experimental model. Administration of EM inhibited leukocyte (neutrophil) accumulation in the middle ear cavity after LPS stimulation. Moreover, EM downregulated L-selectin expression and inhibited interleukin (IL)-8-induced upregulation of Mac-1 on peripheral blood neutrophils. These finding suggests that EM may improve otitis media with effusion by inhibiting neutrophil accumulation in the middle ear cavity through modulating the expression of adhesion molecules L-selectin and Mac-1 on peripheral blood neutrophils.

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Section: Discussionmentioning

confidence: 99%

Effect of Erythromycin on Otitis Media with Effusion in Experimental Rat Model

Enomoto¹,

Ichikawa²,

Nagaoka³

et al. 1998

Acta Oto-Laryngologica

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“…The recruitment and migration of inflammatory cells at sites of mucosal inflammation involve a variety of adhesion molecules [1]. Nonallergic chronic sinusitis is characterized by purulent paranasal sinus effusion and persistent nasal discharge with numerous emigrated neutrophils [2, 3, 4, 5, 6, 7, 8, 9], in additon to the accumulation of eosinophils in the tissue [5, 10, 11, 12, 13]. Tokushiga et al [14]proposed that IL–1β induces the expression of both intercellular adhesion molecule–1 (ICAM–1) and E–selectin on ECs, presumably leading to neutrophil infiltration.…”

Section: Introductionmentioning

confidence: 99%

“…We recently demonstrated that IL–8 generated by emigrated neutrophils and mucosal epithelia plays a pivotal role in the selective neutrophil migration into the sinus effusion [6]. More recently, we revealed that ICAM–1 expression in mucosal microvascular EC was correlated to neutrophil infiltration into the sinus mucosa and contributed to the establishment of the inflammatory cell distribution in patients with nonallergic chronic sinusitis [7]. On the other hand, the driving force for eosinophil accumulation in the sinus mucosa was associated with endothelial vascular cell adhesion molecule–1 (VCAM–1) upregulation induced by TNF–α [12].…”

Section: Introductionmentioning

confidence: 99%

Expression of Adhesion Molecules in Cultured Human Nasal Mucosal Microvascular Endothelial Cells Activated by Interleukin–1â or Tumor Necrosis Factor–·: Effects of Dexamethasone

Yamamoto

Ikeda

Watanabe

et al. 1998

Int Arch Allergy Immunol

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Adhesion molecules of microvascular endothelial cells play a key role in the inflammatory processes involved in nonallergic sinusitis. We investigated the cytokine–regulated expression of intercellular adhesion molecule–1 (ICAM–1), E–selectin and vascular cell adhesion molecule–1 (VCAM–1), and the effect of dexamethasone on these expressions in cultured human nasal microvascular endothelial cells (HNMEC). ICAM–1 was enhanced, and E–selectin and VCAM–1 were induced in a dose–dependent fashion following stimulation with IL–1β or TNF–α. HNMEC differed from human umbilical vein endothelila cells in that (1) maximal upregulation of ICAM–1 expression induced by IL–1β or TNF–α required more time (2) TNF–α was more potent than IL–1β in VCAM–1 expression, and (3) dexamethasone inhibited the upregulation of E–selectin expression alone. These findings contribute to a better understanding of the characteristic features of leukocyte infiltration into inflamed tissue and the effect of glucocorticoid in nonallergic chronic sinusitis.

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“…However, under pathophysiological conditions, other cell types are found to express P-selectin. Notably, it has been reported that epithelial cells from breast carcinomas, 16 from paranasal chronic sinusitis mucosa, 17 and from glomerulonephritis biopsies 18 express P-selectin. This property has been exploited in this study to develop a model system to assess the role of vWF in the sorting of P-selectin and the biogenesis of WPB-like organelles.…”

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confidence: 99%

Assembly of Multimeric von Willebrand Factor Directs Sorting of P-Selectin

Hop

Guilliatt

Daly

et al. 2000

ATVB

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Abstract-We designed a model system to study the role of von Willebrand factor (vWF) in the sorting of P-selectin and the biogenesis of Weibel-Palade body (WPB)-like organelles. For that purpose, a human epithelial cell line (T24) that synthesizes P-selectin mRNA, but which is devoid of vWF mRNA synthesis and storage organelles, was transfected with full-length vWF cDNA or a deletion mutant thereof. Stable transfectants of T24 with full-length vWF cDNA revealed the generation of WPB-like organelles as demonstrated by colocalization of vWF and P-selectin with double-labeling immunofluorescence. In contrast, T24 cells transfected with vWF delD'D3 cDNA, encoding a mutant that is unable to form vWF multimers, displayed only perinuclear vWF staining, whereas no indication was found for the presence of WPB-like organelles. The contents of the organelles in full-length vWF cDNA-transfected T24 cells were released on activation of the protein kinase C pathway, similar to the situation with genuine endothelial cells. The expression of vWF did not affect the biosynthesis of P-selectin, as deduced from the observation that untransfected and vWF cDNA-transfected T24 cells contained the same amount of P-selectin mRNA. We propose that the biosynthesis of multimeric vWF directs the generation of WPB-like organelles, as evidenced by the sequestering and anchoring of P-selectin into these storage granules. Key Words: von Willebrand factor Ⅲ P-selectin Ⅲ Weibel-Palade bodies Ⅲ protein sorting M ultimeric von Willebrand factor (vWF) is an obligatory glycoprotein for the formation of a hemostatic plug. It connects the platelet to the subendothelium that is exposed on injury of the vessel wall. vWF is synthesized by megakaryocytes and vascular endothelial cells. Endothelial cells display 2 secretory routes for newly synthesized vWF, namely, a constitutive and a regulated pathway. 1 Constitutively secreted vWF is rapidly transported in secretory vesicles toward the plasma membrane. This material consists of vWF dimers and low-molecular-weight multimers. Another portion of the newly synthesized vWF is encountered in specific storage organelles, denoted Weibel-Palade bodies (WPBs). These organelles fuse with the plasma membrane on activation of the protein kinase C signal transduction pathway, followed by release of their contents (regulated secretion). 2,3 vWF routed by this pathway consists of high-molecular-weight multimers that are Ϸ2 orders of magnitude more effective in mediating adhesion of platelets to the injured vessel wall than are vWF dimers or low-molecular-weight multimers. 4 The WPBs are considered to be endothelial cell-specific, rod-shaped, electron-dense storage organelles. 5 Apart from vWF multimers and the vWF propolypeptide, 6 -8 the WPB contains a number of other proteins, namely CD63, a transmembrane glycoprotein that is also present in lysosomal membranes of many cell types 9,10 ; interleukin-8 11 ; tissue plasminogen activator 12 ; and the transmembrane receptor P-selectin (also called GMP-140 or PADGEM). 13,14...

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Expression of Adhesion Molecules in Nonallergic Chronic Sinusitis

Cited by 8 publications

References 22 publications

Effect of Erythromycin on Otitis Media with Effusion in Experimental Rat Model

Effect of Erythromycin on Otitis Media with Effusion in Experimental Rat Model

Expression of Adhesion Molecules in Cultured Human Nasal Mucosal Microvascular Endothelial Cells Activated by Interleukin–1â or Tumor Necrosis Factor–·: Effects of Dexamethasone

Assembly of Multimeric von Willebrand Factor Directs Sorting of P-Selectin

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