Expression of Adh8 mRNA is developmentally regulated in Japanese medaka (Oryzias latipes)

Dasmahapatra, Asok K.; Wang, Xueqing; Haasch, Mary L.

doi:10.1016/j.cbpc.2005.01.007

Cited by 23 publications

(25 citation statements)

References 20 publications

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“…However, the expression of these mRNA was developmentally regulated (Dasmahapatra et al, 2005;Wang et al, 2007a,b). In our current experiment the Aldh9 mRNA data obtained by rRT-PCR and qRT-PCR were not identical, but we were still able to demonstrate a significant dose-and developmental stage-specific reduction in these mRNA level by ethanol during medaka embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Methods of animal maintenance, egg collection, RNA, and protein preparation and purification, semi-quantitative relative (rRT-PCR) and quantitative real-time PCR (qRT-PCR) techniques were previously described (Dasmahapatra et al, 2005;Wang et al, 2006Wang et al, , 2007bWu et al, 2008). The major modification made in the present experimental protocol was in the maintenance of medaka embryos during ethanol treatment.…”

Section: Methodsmentioning

confidence: 99%

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Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Khan

Dasmahapatra

2008

Comparative Biochemistry and Physiology Part C: Toxicology &

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Japanese medaka (Oryzias latipes) embryos exposed to ethanol have developed craniofacial, cardiovascular and skeletal defects which can be compared with the phenotypic features of fetal alcohol spectrum disorder (FASD) observed in human. The present experiment was designed to show that the disruption in circulation by ethanol during embryogenesis is a potential cause of FASD. Fertilized eggs were exposed to ethanol (0, 100 and/or 400 mM) for 24 or 48 h at various developmental stages (Iwamatsu stages 4-30) and were analyzed at 6 day post fertilization (dpf). It was observed that controls and the embryos exposed to 100 mM ethanol were in circulating state; however, a significant number of embryos of stages 4-24 exposed to 400 mM ethanol had disrupted circulation. Compared to controls, protein and RNA contents were significantly reduced in noncirculating embryos. Lipid peroxidation (LPO) analysis was made at 3, 6, 24, 48, 96 and 144 hour post fertilization (hpf). LPO was increased with the advancement of morphogenesis; however, ethanol or the circulation status had no effect. We further analyzed alcohol dehydrogenase (Adh 5 and adh8) and aldehyde dehydrogenase (Aldh9A and Aldh1A2) enzyme mRNAs in the embryos exposed to 400 mM ethanol for 24h. A developmental stage specific reduction in these enzyme mRNAs by ethanol was observed. We conclude that ethanol-induced disruption in circulation during embryogenesis is a potential cause of the development of FASD features in medaka.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Khan

Dasmahapatra

2008

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Adh8 mRNA in Japanese Medaka Embryos Dasmahapatra et al (2005) showed that the medaka embryo, like other fish species, expresses Adh mRNA during development. In medaka, Adh5 expression was constitutive throughout development whereas Adh8 mRNA expression was developmentally regulated .…”

Section: Effect Of Ethanol On Expression Of Adh5 Andmentioning

confidence: 99%

Japanese medaka (Oryzias latipes): developmental model for the study of alcohol teratology

Wang

Williams

Haasch

et al. 2006

Birth Defects Research Pt B

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BACKGROUND: Animal models are necessary to investigate the mechanism of alcohol-induced birth defects. We have used Japanese medaka (Oryzias latipes) as a non-mammalian model to elucidate the molecular mechanism(s) of ethanol teratogenesis. METHODS: Medaka eggs, within 1 hr post-fertilization (hpf) were exposed to waterborne ethanol (0-1000 mM) in hatching solution for 48 hr. Embryo development was observed daily until 10 days post-fertilization (dpf). The concentration of embryonic ethanol was determined enzymatically. Cartilage and bones were stained by Alcian blue and calcein, respectively and skeletal and cardiovascular defects were assessed microscopically. Genetic gender of the embryos was determined by PCR. Levels of two isoenzymes of alcohol dehydrogenase (Adh) mRNAs were determined by semi-quantitative and real-time RT-PCR. RESULTS: The concentration of ethanol required to cause 50% mortality (LC 50 ) in 10 dpf embryos was 568 mM, however, the embryo absorbed only 15-20% of the waterborne ethanol at all ethanol concentrations. The length of the lower jaw and calcification in tail fin cartilaginous structures were reduced by ethanol exposure. Active blood circulation was exhibited at 501 hpf in embryos treated with 0-100 mM ethanol; active circulation was delayed and blood clots developed in embryos treated with 200-400 mM ethanol. The deleterious effects of ethanol were not gender-specific. Moreover, ethanol treatment was unable to alter the constitutive expression of either Adh5 or Adh8 mRNA in the medaka embryo. CONCLUSIONS: Preliminary results suggested that embryogenesis in medaka was significantly affected by ethanol exposure. Phenotypic features normally associated with ethanol exposure were similar to that observed in mammalian models of fetal alcohol syndrome. The results further indicated that medaka embryogenesis might be used as an alternative non-mammalian model for investigating specific alterations in gene expression as a means to understand the molecular mechanism(s) of ethanol-induced birth defects.

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“…In mouse, oxidation of retinol to retinaldehyde is ubiquitous as it is catalyzed by ADH3 (class III ADH), which is not tissue-specific; while, is widely expressed throughout development [29]. In medaka, adh5 , an orthologue of mammalian Class III ADH, is also ubiquitously expressed, however, whether ADH5 can induce the RA synthesis system in medaka has not yet been determined [42]. Our results suggest that nsPEF exposure affected the first step of RA synthesis, oxidation of retinol to retinaldehyde, while the exposure did not influence the signal transduction, including RA receptors.…”

Section: Discussionmentioning

confidence: 99%

Nanosecond Pulsed Electric Field Suppresses Development of Eyes and Germ Cells through Blocking Synthesis of Retinoic Acid in Medaka (Oryzias latipes)

et al. 2013

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Application of nanosecond pulsed electric fields (nsPEFs) has attracted rising attention in various scientific fields including medical, pharmacological, and biological sciences, although its effects and molecular mechanisms leading to the effects remain poorly understood. Here, we show that a single, high-intensity (10–30 kV/cm), 60-ns PEF exposure affects gene expression and impairs development of eyes and germ cells in medaka (Oryzias latipes). Exposure of early blastula stage embryos to nsPEF down-regulated the expression of several transcription factors which are essential for eye development, causing abnormal eye formation. Moreover, the majority of the exposed genetic female embryos showed a fewer number of germ cells similar to that of the control (unexposed) genetic male at 9 days post-fertilization (dpf). However, all-trans retinoic acid (atRA) treatment following the exposure rescued proliferation of germ cells and resumption of normal eye development, suggesting that the phenotypes induced by nsPEF are caused by a decrease of retinoic acid levels. These results confirm that nsPEFs induce novel effects during embryogenesis in medaka.

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Expression of Adh8 mRNA is developmentally regulated in Japanese medaka (Oryzias latipes)

Cited by 23 publications

References 20 publications

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Disruption of circulation by ethanol promotes fetal alcohol spectrum disorder (FASD) in medaka (Oryzias latipes) embryogenesis

Japanese medaka (Oryzias latipes): developmental model for the study of alcohol teratology

Nanosecond Pulsed Electric Field Suppresses Development of Eyes and Germ Cells through Blocking Synthesis of Retinoic Acid in Medaka (Oryzias latipes)

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