Expression of ACTH-induced corticosteroid biosynthesis in newborn rat adrenocortical cells cultured in serum-free and carrier protein-free medium containing a cholesterol-?-cyclodextrin complex

Hammami, Mohamed Amen; Maume, G.; Maume, B.F.

doi:10.1007/bf00117706

Cited by 8 publications

(3 citation statements)

References 22 publications

(17 reference statements)

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“…We do not observe a correlation between OTS layer thickness and the contact angles. Figure 5. Advancing water contact angles on HOC22SÍ/SÍ monolayers vs layer number.…”

Section: Resultsmentioning

confidence: 99%

“…f 0 Spectra were recorded as described in note a, Table I, and are of a multilayer sample prepared on the initial monolayer presented in Table I as sample 1. 'The notation [1][2][3][4][5][6][7] means the spectrum of the seven-layer sample; layer eight is the spectrum of the eighth layer obtained by subtraction of the seven-layer spectra from the eight-layer spectra. ' The samples were either those with an ester surface (E) or reduced (R) to the alcohol surface.…”

Section: Resultsmentioning

confidence: 99%

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Formation of multilayers by self-assembly

Ulman²,

1989

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Monolayer and multilayer films were formed by self-assembly of methyl 23-(trichlorosilyl)tricosanoate (1) from organic solution. In agreement with published results, this compound was found to form good quality, close-packed monolayers on silicon surfaces. We have, however, found that films significantly thicker than the three monolayers previously obtained can be formed from continued chemisorption of monolayers of this compound followed by reduction of the surface ester with LiAlH4 in THF to form an "alcohol surface". The quality of monolayer formation in the multilayer films was monitored in detail by ellipsometry, contact angle, and FTIR measurements, and, although generally increasing disorder can be detected, films of up to 25 discrete monolayers can be successfully made. These results indicate that self-assembly is a viable alternative to the Langmuir-Blodgett transfer technique for the construction of relatively thick (O.l-gm scale), ordered, multilayer films.

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“…We do not observe a correlation between OTS layer thickness and the contact angles. Figure 5. Advancing water contact angles on HOC22SÍ/SÍ monolayers vs layer number.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Formation of multilayers by self-assembly

Ulman²,

1989

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“…Delivery and supply of lipids have been shown to be an important role of serum and one of the most difficult functions to replace in chemically defined media. Cyclodextrins, which are cyclic glucose compounds with a hydrophobic interior and a hydrophilic exterior, have been utilized as carriers for fatty acids and cholesterol with some success in serum-free medium formulations (Gorfien et al, 2000;Greenberg-Ofrath et al, 1993;Hammami et al, 1986;Keen and Steward, 1995;Minamoto et al, 1991). Pluronic 1 F-68 (PF-68), which is commonly used in cell culture medium to protect cells from bubble damage (Handa-Corrigan et al, 1989) has also been used to emulsify lipids to allow delivery into aqueous culture media (Maiorella et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Quantitation of interaction of lipids with polymer surfaces in cell culture

Altaras

Eklund

Ranucci

et al. 2006

Biotech & Bioengineering

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As cell culture medium development efforts have progressed towards leaner, serum-free, and chemically defined formulations, it has become increasingly important to ensure that the appropriate concentrations of all nutrients are maintained and delivered at point of use. In light of concurrent efforts to progress to disposable polymeric storage and culture platforms, the characterization and control of medium component interactions with container surfaces can be a key issue in ensuring consistent delivery of these medium formulations. These studies characterize the interactions of lipids with culture surfaces typically encountered in the bioprocess industry using model systems. The extent and kinetics of lipid association with polymeric surfaces were determined using radio-labeled linoleic acid and cholesterol. The effect of methyl-beta-cyclodextrin, a component commonly used to solubilize lipids in culture media, on association kinetics was also examined. In addition, loss of lipids across a sterilizing membrane filter was quantified. We find that there is potential for significant loss of hydrophobic components due to non-specific binding to surfaces at timescales relevant to a typical cell culture process. The extent of loss is dependent on the nature of the hydrophobic component as well as the type of surface. These studies highlight the potential of the extracellular environment to modify medium composition and also emphasize the importance of medium formulation strategies, including those used in the delivery of hydrophobic components. It is noted, however, that the level of loss is very dependent on the specific system including the composition of the culture medium used.

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Animal Cell Culture Media

Tsao¹,

Gould

Robinson

2000

Encyclopedia of Cell Technology

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Serum Chemically Defined Basal Media and Minimal Essential Media (MEM) Serum‐Free Media Protein‐Free Media Medium Stability and Storage Medium Development for Large‐Scale Production Development of Serum‐Free Media: A Case Study Bibliography At the start of the twentieth century, researchers were trying to grow mammalian cells derived from biopsies, including cancer cells, fetal cells, and primary cells from various tissues. Since that time, medium development has followed one of two approaches; researchers have made use of biological fluids, including serum, embryo extracts, and protein digests, or they have studied those fluids and tried to find simpler substitutes for them. The following sections describe the basic components of cell culture media discovered by both of these approaches. These are ( 1 ) serum and its components, functions and processing; ( 2 ) chemically defined basal media that supplement serum for cell growth; ( 3 ) serum‐free media (or serum‐reconstitution); ( 4 ) protein‐free media (or serum‐protein replacement); ( 5 ) medium stability and storage; ( 6 ) medium development for large‐scale production; and ( 7 ) a case study for developing a serum‐free medium.

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Expression of ACTH-induced corticosteroid biosynthesis in newborn rat adrenocortical cells cultured in serum-free and carrier protein-free medium containing a cholesterol-?-cyclodextrin complex

Cited by 8 publications

References 22 publications

Formation of multilayers by self-assembly

Formation of multilayers by self-assembly

Quantitation of interaction of lipids with polymer surfaces in cell culture

Animal Cell Culture Media

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