Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

Rodríguez, María Verónica; Mendiondo, Guillermina M.; Maskin, Laura; Gudesblat, Gustavo E.; Iusem, Norberto D.; Benech‐Arnold, Roberto L.

doi:10.1093/aob/mcp184

Cited by 42 publications

(51 citation statements)

References 33 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of ABI5 in dormancy induction is discussed (Finkelstein et al 2008) mostly because of the lack of such research concerning Arabidopsis seeds (Dekkers et al 2016). Research with orthologues, however, has confirmed that ABI5 homologues are more highly expressed in dormant sorghum cultivars (Rodríguez et al 2009). Despite this, Finkelstein et al (2008) concluded that further studies were required to confirm the role of ABI5 in seed dormancy.…”

Section: Introductionmentioning

confidence: 99%

Signalling regulators of abscisic and gibberellic acid pathways are involved in dormancy breaking of Norway maple (Acer platanoides L.) seeds

2017

View full text Add to dashboard Cite

Physiological dormancy is a characteristic of the seeds of plants that are exposed to adverse harsh environmental conditions. Many tree seeds from the northern hemisphere have acquired deep dormancy as an adaptation to the winter period. Such kinds of dormancy can be removed by cold stratification. This physiological process is regulated through abscisic and gibberellic acids signal transduction, in which ABI5, 14-3-3 and RGL2 have a negative response to cold stratification and cause dormancy breaking. Our study is, to our knowledge, the first to report tissue localisation of ABI5 and RGL2 in deeply physiologically dormant seeds. Localisation of these proteins differs in time (weeks of stratification) and space (anatomy of the embryo root). Studies showed that changes occurred on three levels: (1) tissue, as the fluorescence signal throughout the weeks of stratification was localised in different regions of the embryo axes, and these changes were associated with changes in development regulation of the individual regions; (2) cell, either in nucleus or in cytoplasm, involving regulation of gene expression, and synthesis and inactivation in cytoplasm; and (3) organelle, specifically in nuclei/nucleoli, indicating transcription regulation of the specific genes. At the end of stratification, when dormancy is broken, ABI5 and RGL2 were not noticeable in the cells of the apical meristem. ABI5 likely blocked germination through inhibition of meristem activity, whereas RGL2 through blocking of procambial cell differentiation. We speculate that similar molecular and cellular mechanisms exist among other seeds characterised by physiological dormancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Signalling regulators of abscisic and gibberellic acid pathways are involved in dormancy breaking of Norway maple (Acer platanoides L.) seeds

2017

View full text Add to dashboard Cite

show abstract

“…In this scenario, new mapping studies are needed to narrow these QTL and carry out future fine mapping analysis to identify individual genes. Of particular interest was the co-localisation of genes SbABI3/VP1 and SbGA20ox3 within qGI-3, taking into account that ABA sensitivity and GA metabolism are the most relevant physiological components to explain differences in seed dormancy behaviour for the parental lines used in this work (Steinbach et al 1995;Gualano et al 2007;Rodríguez et al 2009Rodríguez et al , 2012. On the other hand, the detected QTL do not colocalise with known wheat PHS QTL and seem to result from different genes.…”

Section: Discussionmentioning

confidence: 99%

“…Other features, such as the panicle architecture, glumes or glumellae presence and seed tannin content, which could actually affect the relationship between dormancy and PHS susceptibility, have been shown not to be involved in IS9530/RedlandB2 PHS behaviour (Steinbach et al 1995). Premature release from dormancy in RedlandB2 grains has been ascribed to a reduced sensitivity of their embryos to the germination inhibitory action of abscisic acid (ABA) and an abnormal accumulation of active gibberellins (GAs) (Steinbach et al 1995(Steinbach et al , 1997Rodríguez et al 2009Rodríguez et al , 2012. Both processes (faulty ABA sensitivity and GA inactivation) have been found to be differentially regulated in this line at the transcriptional level of genes involved in ABA signalling and GA catabolism (Rodríguez et al 2009(Rodríguez et al , 2012.…”

Section: Introductionmentioning

confidence: 99%

“…Premature release from dormancy in RedlandB2 grains has been ascribed to a reduced sensitivity of their embryos to the germination inhibitory action of abscisic acid (ABA) and an abnormal accumulation of active gibberellins (GAs) (Steinbach et al 1995(Steinbach et al , 1997Rodríguez et al 2009Rodríguez et al , 2012. Both processes (faulty ABA sensitivity and GA inactivation) have been found to be differentially regulated in this line at the transcriptional level of genes involved in ABA signalling and GA catabolism (Rodríguez et al 2009(Rodríguez et al , 2012. QTL searches for PHS resistance in immature sorghum grains were attempted previously by Lijavetzky et al (2000) using a RedlandB2 x IS9530 segregating population.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seed dormancy QTL identification across a Sorghum bicolor segregating population

et al. 2016

View full text Add to dashboard Cite

Pre-harvest sprouting (PHS) in Sorghum bicolor is one of the main constrains for its production in the central region of Argentina, as grain maturation often coincides with rainy or high environmental humidity conditions. The obtention of more dormant genotypes with higher PHS resistance has always been a desirable trait for breeders but the typical quantitative nature of seed dormancy makes its manipulation difficult through classical breeding. Dissecting this quantitative variability into quantitative trait loci (QTL) is a main concern especially in cereal species. In this work, a sorghum segregating population including 190 families was genotyped with microsatellite markers and the SbABI5 candidate gene. A genetic map encompassing 96 markers and a total length of 1331 cM was built. Seed dormancy was phenotyped in F 3 and F 4 panicles in two contrasting Argentinean environments (Castelar and Manfredi). Six seed dormancy QTL for mature grains were identified (qGI-1, qGI-3, qGI-4, qGI-6, qGI-7 and qGI-9) with the aid of QTL Cartographer and QTLNetwork, three of them (qGI-3, qGI-7 and qGI-9) being co-localised by both approaches. No epistasis was detected for the identified QTL but QTL-byenvironment interaction was significant for qGI-7 and qGI-9. Interestingly, seed dormancy candidate genes Ruth A. Heinz and Roberto L. Benech-Arnold have contributed equally to this study.Electronic supplementary material The online version of this article (

show abstract

“…Application of methyl jasmonate reduces dormancy and ABA content in imbibed wheat grains and this is mediated via a reduction in TaNCED1 and an increase in TaABA8 0 OH-1 expression (Jacobsen et al, 2013). While maintenance of high ABA concentrations after imbibition is required for the expression of dormancy in the barley grain, this is not the case in some wheat and sorghum genotypes (Rodríguez et al, 2009;. In grain sorghum (S. bicolor), coat-imposed dormancy in two inbred lines with contrasting dormancy is not related to ABA content, which decreases similarly upon grain imbibition (Gualano et al, 2007;Rodríguez et al, 2009).…”

Section: Hormones In the Expression Of Dormancy In The Imbibed Grainmentioning

confidence: 99%

Dormancy in cereals (not too much, not so little): about the mechanisms behind this trait

et al. 2015

View full text Add to dashboard Cite

As in other cultivated species, dormancy can be seen as a problem in cereal production, either due to its short duration or to its long persistence. Indeed, cereal crops lacking enough dormancy at harvest can be exposed to pre-harvest sprouting damage, while a long-lasting dormancy can interfere with processes that rely on rapid germination, such as malting or the emergence of a uniform crop. Because the ancestors of cereal species evolved under very diverse environments worldwide, different mechanisms have arisen as a way of sensing an appropriate germination environment (a crucial factor for winter or summer annuals such as cereals). In addition, different species (and even different varieties within the same species) display diverse grain morphology, allowing some structures to impose dormancy in some cereals but not in others. As in seeds from many other species, the antagonism between the plant hormones abscisic acid and gibberellins is instrumental in cereal grains for the inception, expression, release and re-induction of dormancy. However, the way in which this antagonism operates is different for the various species and involves different molecular steps as regulatory sites. Environmental signals (i.e. temperature, light quality and quantity, oxygen levels) can modulate this hormonal control of dormancy differently, depending on the species. The practical implications of knowledge accumulated in this field are discussed.

show abstract

Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

Cited by 42 publications

References 33 publications

Signalling regulators of abscisic and gibberellic acid pathways are involved in dormancy breaking of Norway maple (Acer platanoides L.) seeds

Signalling regulators of abscisic and gibberellic acid pathways are involved in dormancy breaking of Norway maple (Acer platanoides L.) seeds

Seed dormancy QTL identification across a Sorghum bicolor segregating population

Dormancy in cereals (not too much, not so little): about the mechanisms behind this trait

Contact Info

Product

Resources

About