Expression of a wild eggplant ribosomal protein L13a in potato enhances resistance to Verticillium dahliae

Liu, Yang; Xie, Conghua; Li, Wang; Zhang, Ruijie; Jue, Dengwei; Yang, Qing

doi:10.1007/s11240-013-0365-4

Cited by 33 publications

(28 citation statements)

References 45 publications

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“…The response to environmental stimuli and ribosomal proteins were also reported in low temperatures conditions, inducing the expression of the RPS6, RPS13 and RPL37 genes in soybean plants (Kim et al, 2004); which has been reported for the homologs RPL13 and C34 in Brassica and E. coli (Sáez-Vázquez et al, 2000; Tanaka et al, 2001); This fact was also evident in the vanilla transcriptome against Fusarium , which indicates the central role of RPL13 in the response to stress in plants, both biotic and abiotic. And as mentioned above, the remarkably overexpression of RPL13 resulted in the tolerance of transgenic potato plants, against the pathogenic fungus Verticillum dahliae , with an increase in the expression of defense genes and antioxidant enzymes (Yang et al, 2013).…”

Section: Discussionmentioning

confidence: 88%

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Cruz

Adame-García

Gregorio-Jorge

et al. 2019

Preprint

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BackgroundUpon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptomical changes must occur during interactions with pathogens, this causing the triggering of pathogen- and plant-derived molecules. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that finally leads to plant death. To further investigate how vanilla plants respond at the transcriptional level upon infection with F.oxysporum f. sp. vanillae, we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection.ResultsAnalysis of global gene expression profiles indicated that a major transcriptional change occurs at 2 dpi, in comparison to 10 dpi, whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts.ConclusionsWe propose that the plant-pathogen interaction at early stages causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla, where ribosomal proteins and regulation of the translational mechanism are critical. These are insights into the defense responses of V. planifolia Jacks., providing the basis for the understanding of the plant early response towards biotic stress.

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Section: Discussionmentioning

confidence: 88%

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Cruz

Adame-García

Gregorio-Jorge

et al. 2019

Preprint

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“…Plastidspecific ribosomal protein 2 (PSRP2), with RNA chaperone activity, participates as a negative regulator in seed germination and seedling growth under abiotic stress conditions and thus has a role in the abiotic stress response (16). Overexpressed StoL13a in potato (Solanum tuberosum) was shown to enhance disease resistance against Verticillium dahliae infection in transgenic potato plants (17). An antimicrobial peptide purified from skin secretions and epithelial cells of rainbow trout (likely the 40S ribosomal protein S30) has a protective function against intracellular or extracellular pathogens (18).…”

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confidence: 99%

Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus

Liu

Xie

et al. 2014

Appl Environ Microbiol

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bRibosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mRNA leading to protein synthesis. However, certain ribosomal proteins moonlight in the development and differentiation of organisms. In this study, the ribosomal protein L44 (RPL44), associated with salt resistance, was screened from the halophilic fungus Aspergillus glaucus (AgRPL44), and its activity was investigated in Saccharomyces cerevisiae and Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one of the proteins of the large ribosomal subunit 60S. Expression of AgRPL44 was upregulated via treatment with salt, sorbitol, or heavy metals to demonstrate its response to osmotic stress. A homologous sequence from the model fungus Magnaporthe oryzae, MoRPL44, was cloned and compared with AgRPL44 in a yeast expression system. The results indicated that yeast cells with overexpressed AgRPL44 were more resistant to salt, drought, and heavy metals than were yeast cells expressing MoRPL44 at a similar level of stress. When AgRPL44 was introduced into M. oryzae, the transformants displayed obviously enhanced tolerance to salt and drought, indicating the potential value of AgRPL44 for genetic applications. To verify the value of its application in plants, tobacco was transformed with AgRPL44, and the results were similar. Taken together, we conclude that AgRPL44 supports abiotic stress resistance and may have value for genetic application.

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“…Disease intensity was graded using the method of Yang et al (2013) as follows: grade 0, no disease dots on the leaves of the plants; grade 1, one to two withered leaves; grade 2, three to four withered leaves; grade 3, most leaves withered; and grade 4, the plant is withered nearly to death. A disease index was calculated according to the disease grading criterion of grades 0-4 using the formula: disease index = [R (number of diseased plants 9 disease grade)/ (total number of investigated plants 9 the highest disease grade)] 9 100.…”

Section: Evaluation Of Disease Resistance Of Transgenic Tobacco Plantsmentioning

confidence: 99%

Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae

Liu

Shi

et al. 2015

Plant Biotechnol Rep

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Plant P450s play an important role in plant response to environmental stimuli, especially to pathogen challenge, besides leading to the biosynthesis of secondary metabolites. In this work, a Verticillium dahliae-induced cDNA sequence was cloned from wild eggplant Solanum torvum and designated StoCYP77A2. StoCYP77A2 encodes a 511-amino acid peptide that shows sequence homology to the plant CYP77 family. StoCYP77A2 is expressed in a tissue-specific manner with the highest expression level in stem. The expression level of StoCYP77A2 was increased in wild eggplants which were infected with Verticillium dahlia. We expressed StoCYP77A2 in V. dahliae-sensitive tobacco, and the transgenic tobacco plants were more resistant to V. dahliae infection than the wild type. Protein extraction of transgenic tobacco showed strong antifungal activity against V. dahliae, indicating that StoCYP77A2 may be involved in the synthesis of some antimicrobial compounds. These results suggest that StoCYP77A2 plays a role in plant defense to V. dahliae infection.

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Expression of a wild eggplant ribosomal protein L13a in potato enhances resistance to Verticillium dahliae

Cited by 33 publications

References 45 publications

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus

Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae

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