Expression of a Vacuolar Na+/H+ Antiporter Gene of Alfalfa Enhances Salinity Tolerance in Transgenic Arabidopsis

An, Bao-Yan; Luo, Yan; Li, Jiarui; Qiao, Weihua; Zhang, Xiansheng; Gao, Xin-Qi

doi:10.1016/s1875-2780(08)60022-x

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…Several reports on improvement of salt tolerance through overexpression of vacuolar NHX s in agriculturally important but glycophytic crops implicate a pivotal function of the NHX s in intracellular compartmentalization of Na + and salt tolerance [3], [12]. In legumes, NHX1 has been reported in Glycine max [13], Medicago sativa [14], Trifolium repens [15], Lotus tenuis [16], Caragana korshinskii [17] and recently by our lab, in Vigna unguiculata (GenBank Acc. No.…”

Section: Introductionmentioning

confidence: 81%

Cloning and Functional Characterization of a Vacuolar Na+/H+ Antiporter Gene from Mungbean (VrNHX1) and Its Ectopic Expression Enhanced Salt Tolerance in Arabidopsis thaliana

et al. 2014

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Plant vacuolar NHX exchangers play a significant role in adaption to salt stress by compartmentalizing excess cytosolic Na+ into vacuoles and maintaining cellular homeostasis and ionic equilibrium. We cloned an orthologue of the vacuolar Na+/H+ antiporter gene, VrNHX1 from mungbean (Vigna radiata), an important Asiatic grain legume. The VrNHX1 (Genbank Accession number JN656211.1) contains 2095 nucleotides with an open reading frame of 1629 nucleotides encoding a predicted protein of 542 amino acids with a deduced molecular mass of 59.6 kDa. The consensus amiloride binding motif (84LFFIYLLPPI93) was observed in the third putative transmembrane domain of VrNHX1. Bioinformatic and phylogenetic analysis clearly suggested that VrNHX1 had high similarity to those of orthologs belonging to Class-I clade of plant NHX exchangers in leguminous crops. VrNHX1 could be strongly induced by salt stress in mungbean as the expression in roots significantly increased in presence of 200 mM NaCl with concomitant accumulation of total [Na+]. Induction of VrNHX1 was also observed under cold and dehydration stress, indicating a possible cross talk between various abiotic stresses. Heterologous expression in salt sensitive yeast mutant AXT3 complemented for the loss of yeast vacuolar NHX1 under NaCl, KCl and LiCl stress indicating that VrNHX1 was the orthologue of ScNHX1. Further, AXT3 cells expressing VrNHX1 survived under low pH environment and displayed vacuolar alkalinization analyzed using pH sensitive fluorescent dye BCECF-AM. The constitutive and stress inducible expression of VrNHX1 resulted in enhanced salt tolerance in transgenic Arabidopsis thaliana lines. Our work suggested that VrNHX1 was a salt tolerance determinant in mungbean.

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Section: Introductionmentioning

confidence: 81%

Cloning and Functional Characterization of a Vacuolar Na+/H+ Antiporter Gene from Mungbean (VrNHX1) and Its Ectopic Expression Enhanced Salt Tolerance in Arabidopsis thaliana

et al. 2014

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“…Na + /H + antiporters are ubiquitous membrane proteins that mediate Na + homeostasis and catalyze the exchange of Na + for H + across membranes (Apse et al, 1999). Recently, many Na + /H + antiporter genes have been identified and characterized in various plant species, including Arbidopsis thaliana (Apse et al, 1999), Oryza sativa (Fukuda et al, 1999), alfalfa (An et al, 2008), Salsola soda , Chrysanthemum crassum (Song et al, 2012), Cucumis sativus (Wang et al, 2013), and Zea mays (Pitann et al, 2013). The primary function conferring salt tolerance of these genes had been manifested by transgenic events in many plants (Asif et al, 2011;Tian et al, 2011;Uliaie et al, 2012;Jiang et al, 2012;Yarra et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Salicornia europaea L. Na+/H+ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.)

Zhang¹,

Niu²,

Huridu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na + /H + antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyses. Of 197 plants that were obtained after transformation, 36 were positive by PCR analysis using 2 primer pairs for the CaMV35S-SeNHX1 and SeNHX1-Nos fragments; 6 plants survived in a greenhouse. RT-PCR analysis revealed that SeNHX1 was expressed in 5 plants. The resultant transgenic alfalfa had better salt tolerance. After stress treatment for 21 days with 0.6% NaCl, the chlorophyll and MDA contents in transgenic plants were lower, but proline content and SOD, POD, and CAT activities were higher than those in wild-type plants. These results suggest that the salt tolerance of transgenic alfalfa was improved by the overexpression of the SeNHX1 gene.

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Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis

et al. 2023

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An effective strategy for increasing the productivity of major crops under salt stress conditions is the development of transgenics that harbor genes responsible for salinity tolerance. Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the biosynthesis of the osmoprotectant, glycine betaine (GB), and osmotic balance in plants, and several plants transformed with BADH have shown signi cant improvements in salt and drought tolerance. However, very few eld-tested transgenic cultivars have been reported, as most of the transgenic studies are limited to laboratory or green house experiments. In this study, we demonstrated through eld experiments that BADHfrom Atriplex hortensis (AhBADH) confers salt tolerance when transformed into soybean (Glycine max L.). AhBADH was successfully introduced into soybean by Agrobacterium mediated transformation. A total of 256 transgenic plants were obtained, out of which 47 lines showed signi cant enhancement of salt tolerance compared to non-transgenic control plants. Molecular analyses of the transgenic line TL7 with the highest salt tolerance exhibited stable inheritance and expression of AhBADH in progenies with a single copy insertion. TL7 exhibited stable enhanced salt tolerance and improved agronomic traits when subjected to 300mM NaCl treatment. Currently, the transgenic line TL7 with stable enhanced salt tolerance, which has been cleared for environmental release, is under biosafety assessment. TL7 stably expressing AhBADH could then be applied in commercial breeding experiments in order to genetically improve salt tolerance in soybean. Key MessageThe salt-tolerance of transgenic soybean cleared for environmental release was improved by stable overexpression of AhBADH gene from Atriplex hortensis, which was demonstrated through molecular analysis and eld experiments.

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Expression of a Vacuolar Na+/H+ Antiporter Gene of Alfalfa Enhances Salinity Tolerance in Transgenic Arabidopsis

Cited by 9 publications

References 23 publications

Cloning and Functional Characterization of a Vacuolar Na+/H+ Antiporter Gene from Mungbean (VrNHX1) and Its Ectopic Expression Enhanced Salt Tolerance in Arabidopsis thaliana

Cloning and Functional Characterization of a Vacuolar Na+/H+ Antiporter Gene from Mungbean (VrNHX1) and Its Ectopic Expression Enhanced Salt Tolerance in Arabidopsis thaliana

Salicornia europaea L. Na+/H+ antiporter gene improves salt tolerance in transgenic alfalfa (Medicago sativa L.)

Improved salt-tolerance of transgenic soybean by stable over-expression of AhBADH gene from Atriplex hortensis

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