Expression of a rice GLP in Medicago truncatula exerting pleiotropic effects on resistance against Fusarium oxysporum through enhancing FeSOD-like activity

Sultana, Tasawar; Deeba, Farah; Naz, Farah; Rose, Ray J.; Naqvi, Syed Muhammad Saqlan

doi:10.1007/s11738-016-2273-9

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…GLPs with SOD activity from wheat and barley have long been known to provide resistance against Blumeria graminis 49 . Recently, more defense-related GLP SODs have been found in heterologous systems such as rice 13 , pepper 34 , sunflower 31 , and rape 50 . In the present study, we obtained that GhGLP2 protein significantly restricted spore germination of the tested V. dahliae and F. oxysporum in a dose-dependent manner (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide dismutase (SOD; EC 1.15.1.1), the first line of defense against ROS 28 , is the major scavenger of O 2 in this system. It has an effect on restricting the ROS-dependent damage and protecting cells from the oxidative burst by dismutation of O 2 to H 2 O 2 and O 2 and often correlated with increased plant resistance to biotic and abiotic stresses 13 . Some GLPs has been proven to be SODs and against oxidative stress by ROS detoxification 14,29,30 .…”

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confidence: 99%

“…BvGLP1 confers resistance against Verticillium longisporum and Rhizoctonia solani, with elevated levels of H 2 O 2 , by constitutively triggering the expression of several plant defense-related proteins (PR-1, PR-2, PR-3, PR-4, and PDF-1.2) 35 . OsRGLP1 provides protection against F. oxysporum that may involve the direct influence of H 2 O 2 on salicylic acid (SA) and jasmonic acid (JA) signaling pathways, leading to the activation of defense-related genes 13 .…”

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Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

Pei

Zhu

Jia

et al. 2020

Sci Rep

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Germin-like proteins (GLps) are a diverse and ubiquitous family of plant glycoproteins belonging to the cupin super family; they play considerable roles in plant responses against various abiotic and biotic stresses. Here, we provide evidence that GLP2 protein from cotton (Gossypium hirsutum) functions in plant defense responses against Verticillium dahliae, Fusarium oxysporum and oxidative stress. Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity and inhibits spore germination of pathogens. Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition. transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum, with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves. The enhanced tolerance of GhGLP2-transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 o 2 production. further, the expression of several defense-related genes (PDF1.2, LOX2, and VSP1) or oxidative stress-related genes (RbohD, RbohF) was triggered by GhGLP2. Thus, our results confirmed the involvement of GhGLP2 in plant defense response against Verticillium and Fusarium wilt pathogens and stress conditions. Cotton (Gossypium hirsutum L.) is an important fiber crop that is considered the backbone of the global fiber economy 1. Verticillium and Fusarium wilt are caused by Verticillium dahliae and Fusarium oxysporum, respectively, which are soil-borne pathogenic fungi that present major constraints to the production of cotton 2. Verticillium wilt is a notorious and devastating disease of cotton 3 , and it occurs before the squaring stage and peaks in the boll-setting stage, causing necrotic areas on the leaves, wilting, and discoloration of the vascular tissues 4. The disease symptoms of Fusarium wilt initiate and peak at the seedling and squaring stages, respectively, showing necrotic patches between the main veins and leaf detachment from the stem 5. Due to diverse factors, such as the climate, pathogen population structures, and cultivar susceptibility, the currently available control measures for these two diseases are not adequate 6. Thus, research on the cultivation of resistant cotton plants, by finding novel disease-resistant genes against these soil-borne fungal species, is consequently, of great importance. Previous studies have suggested that extracellular germins and germin-like proteins (GLPs) could be induced by a range of abiotic or biotic stresses, such as herbivores 7,8 , drought 9,10 , salinity 8,11 and they are considered to be the pathogenesis-related proteins (PRs) 16 family due to their disease resistance property 12-14. Germins and GLPs were first characterized in wheat (Triticum aestivum) and constitute a large plant gene family 15. They occur as water-solub...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

Pei

Zhu

Jia

et al. 2020

Sci Rep

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“…Many GLPs are localized in the cell wall and function as cofactors for cell wall reinforcement by facilitating the cross-linking of plant cell wall components during the formation of papillae, which renders them resistant to infection; this process involves the generation of H 2 O 2 because of their SOD or OXO activity (Wei et al, 1998; Liu Q. et al, 2016; Zhang et al, 2017). SOD is responsible for the dismutation of superoxide to H 2 O 2 and O 2 and the protection of cells from oxidative burst (Sultana et al, 2016). H 2 O 2 can act as a signaling molecule for the induction of systemic acquired resistance of non-inoculated tissues (Tran et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…lilii infection than did the wild-type plants (Zhang et al, 2017). Furthermore, the OsRGLP1 gene was highly expressed in Medicago truncatula transformed lines and provided protection against F. oxysporum (Sultana et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

GhABP19, a Novel Germin-Like Protein From Gossypium hirsutum, Plays an Important Role in the Regulation of Resistance to Verticillium and Fusarium Wilt Pathogens

Pei

Zhu

et al. 2019

Front. Plant Sci.

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Germin-like proteins (GLPs) are water-soluble plant glycoproteins belonging to the cupin superfamily. The important role of GLPs in plant responses against various abiotic and biotic stresses, especially pathogens, is well validated. However, little is known about cotton GLPs in relation to fungal pathogens. Here, a novel GLP gene was isolated from Gossypium hirsutum and designated as GhABP19 . The expression of GhABP19 was upregulated in cotton plants inoculated with Verticillium dahliae and Fusarium oxysporum and in response to treatment with jasmonic acid (JA) but was suppressed in response to salicylic acid treatment. A relatively small transient increase in GhABP19 was seen in H 2 O 2 treated samples. The three-dimensional structure prediction of the GhABP19 protein indicated that the protein has three histidine and one glutamate residues responsible for metal ion binding and superoxide dismutase (SOD) activity. Purified recombinant GhABP19 exhibits SOD activity and could inhibit growth of V. dahliae , F. oxysporum , Rhizoctonia solani , Botrytis cinerea , and Valsa mali in vitro . To further verify the role of GhABP19 in fungal resistance, GhABP19 -overexpressing Arabidopsis plants and GhABP19 -silenced cotton plants were developed. GhABP19-transgenic Arabidopsis lines showed much stronger resistance to V. dahliae and F. oxysporum infection than control (empty vector) plants did. On the contrary, silencing of GhABP19 in cotton conferred enhanced susceptibility to fungal pathogens, which resulted in necrosis and wilt on leaves and vascular discoloration in GhABP19 -silenced cotton plants. The H 2 O 2 content and endogenous SOD activity were affected by GhABP19 expression levels in Arabidopsis and cotton plants after inoculation with V. dahliae and F. oxysporum , respectively. Furthermore, GhABP19 overexpression or silencing resulted in activation or suppression of JA-mediated signaling, respectively. Thus, GhABP19 plays important roles in the regulation of resistance to verticillium and fusarium wilt in plants. These modulatory roles were exerted by its SOD activity and ability to activate the JA pathway. All results suggest that GhABP19 was involved in plant disease resistance.

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The J emalong 2HA line used for Medicago truncatula transformation: hormonology and epigenetics

Rose

Song

2019

The Model Legume Medicago Truncatula

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Expression of a rice GLP in Medicago truncatula exerting pleiotropic effects on resistance against Fusarium oxysporum through enhancing FeSOD-like activity

Cited by 11 publications

References 40 publications

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

GhABP19, a Novel Germin-Like Protein From Gossypium hirsutum, Plays an Important Role in the Regulation of Resistance to Verticillium and Fusarium Wilt Pathogens

The J emalong 2HA line used for Medicago truncatula transformation: hormonology and epigenetics

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