Expression of a ribosome inactivating protein (curcin 2) inJatropha curcas is induced by stress

Qin, Wei; Huang, Mingxing; Xu, Ying; Xin-shen, Zhang; Chen, Fang

doi:10.1007/bf02703672

Cited by 51 publications

(21 citation statements)

References 14 publications

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“…Transcripts for curcin 2 [20] were not present. Unlike ricin from castor, the type-I RIPs of J. curcas lack a lectin cell binding domain and are thus thought to be only mildly cytotoxic upon ingestion [21,22].…”

Section: Ribosome-inactivating Proteins Of J Curcasmentioning

confidence: 86%

Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing

King

Graham

2011

Bioenerg. Res.

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Jatropha curcas L. has received much attention recently as a potential oilseed crop for the production of renewable oil. Despite the interest in this crop, relatively little is known on the molecular biology of this species compared with more established oilseed crops. To gain a more detailed understanding of the processes involved in deposition of oil and protein within Jatropha seeds, we conducted high-throughput sequencing analysis of the transcriptome of developing J. curcas seeds using 454 sequencing. A single sequencing run yielded 195,692 sequences (46 Mbp) of raw sequence data. Assembly of this sequence data produced 12,419 contigs and 17,333 singletons. BLASTX searches of the contigs revealed that storage proteins were the most abundant transcripts. Oleosins, ribosomal proteins, metallothioneins and late embryogenesis abundant proteins were also highly represented. Curcin, a type-I ribosome-inactivating protein, accounted for 0.7% of the transcriptome. No transcripts for type-II ribosome-inactivating proteins were found, suggesting that these are not present in the seeds of J. curcas. To test the power of 454 sequencing compared to conventional gene sequencing as a tool for gene discovery, a search of the homologues for genes involved in the conversion of sucrose to triacylglycerol was conducted. Hits for all the known genes in this process were obtained. Pyrosequencing of the J. curcas developing seed transcriptome has provided a valuable increase in the amount of sequence data currently available for this species. The sequence data will be of great use to those engaged in J. curcas research and crop improvement.

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Section: Ribosome-inactivating Proteins Of J Curcasmentioning

confidence: 86%

Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing

King

Graham

2011

Bioenerg. Res.

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“…Nonetheless, the hypothesis of a defensive role is supported by several lines of experimental evidence. The expression of RIP genes can be regulated by biotic stress, including viral [10] or fungal [11,12] infections, and during plant senescence [13]. The proposed mechanisms for the RIP antimicrobial activity are quite controversial in literature; some authors identified the host ribosomes as the main target of endogenous RIPs, while others claimed that the defense mechanism may be exerted by direct interaction of RIP with invading pathogens (reviewed in [9]).…”

Section: Introductionmentioning

confidence: 99%

Protein Synthesis Inhibition Activity by Strawberry Tissue Protein Extracts during Plant Life Cycle and under Biotic and Abiotic Stresses

Polito

Bortolotti

Mercatelli

et al. 2013

IJMS

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Ribosome-inactivating proteins (RIPs), enzymes that are widely distributed in the plant kingdom, inhibit protein synthesis by depurinating rRNA and many other polynucleotidic substrates. Although RIPs show antiviral, antifungal, and insecticidal activities, their biological and physiological roles are not completely understood. Additionally, it has been described that RIP expression is augmented under stressful conditions. In this study, we evaluated protein synthesis inhibition activity in partially purified basic proteins (hereafter referred to as RIP activity) from tissue extracts of Fragaria × ananassa (strawberry) cultivars with low (Dora) and high (Record) tolerance to root pathogens and fructification stress. Association between the presence of RIP activity and the crop management (organic or integrated soil), growth stage (quiescence, flowering, and fructification), and exogenous stress (drought) were investigated. RIP activity was found in every tissue tested (roots, rhizomes, leaves, buds, flowers, and fruits) and under each tested condition. However, significant differences in RIP distribution were observed depending on the soil and growth stage, and an increase in RIP activity was found in the leaves of drought-stressed plants. These results suggest that RIP expression and activity could represent a response mechanism against biotic and abiotic stresses and could be a useful tool in selecting stress-resistant strawberry genotypes.

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“…Jatropha curcas is a well-known and mass cultivated multipurpose plant with immense medicinal value [22][23][24] . Felke 25 isolated the toxic principle curcin, from the seeds of this plant which is a type 1 RIP possessing strong inhibitory activity on protein synthesis 1 along with N-glycosidase and antitumor activities 4,[26][27][28] . Recent works have demonstrated the cancer directed therapeutic potential of this RIP in conjunction with peptides and nanoparticles 29,30 , additionally emphasizing the compatibility of curcin to collaborate with various delivery strategies without compromising on its activity.…”

mentioning

confidence: 99%

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

Mohamed

Veeranarayanan

Minegishi

et al. 2014

Sci Rep

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Curcin, a type 1 ribosome inactivating protein (RIP) is investigated here for its cellular competence on six mammalian cell lines. Cells exposed to curcin (100 mg/ml) for 72 h exhibited significant cellular metabolic arrest, with the cancer cell lines being more sensitive. The viability assessment of the cancer cells in a 3D cell culture based assay revealed highly restricted sprouting and proliferation with near to complete dead cell population. Prominent mitochondrial dysfunction, elevated reactive oxygen species levels, nuclear degeneration, structural/mechanical destabilization and suppression of defense mechanisms were imminent with the RIP treated cells. Expression levels of nuclear factor kB (NF-kB), cytoskeletal focal adhesion kinases (FAK) and vinculin were significantly diminished. Vital cellular organelles as nucleus, mitochondria and actin were severely incapacitated on RIP exposure resulting in multimodal apoptosis and necrosis. The ability of curcin to impart comprehensive shutdown of the cells, especially cancer cells, complemented with its hemocompatibility, opens up possibilities of utilizing this ribotoxin as a prospective therapeutic candidate against cancers of diverse origins.

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Expression of a ribosome inactivating protein (curcin 2) inJatropha curcas is induced by stress

Cited by 51 publications

References 14 publications

Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing

Profiling the Developing Jatropha curcas L. Seed Transcriptome by Pyrosequencing

Protein Synthesis Inhibition Activity by Strawberry Tissue Protein Extracts during Plant Life Cycle and under Biotic and Abiotic Stresses

Cytological and Subcellular Response of Cells Exposed to the Type-1 RIP Curcin and its Hemocompatibility Analysis

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