Expression of a Plant Cell Wall Invertase in Roots of Arabidopsis Leads to Early Flowering and an Increase in Whole Plant Biomass

Schweinichen, C von; Büttner, Michael

doi:10.1055/s-2005-865894

Cited by 25 publications

(19 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, transgenic tobacco plants expressing the apoplastlocated yeast invertase with a seed-specific promoter did not show increased accumulation of storage products in the oilseeds, even with additional overexpression of hexokinase, indicating no change in the sink strength of these storage sinks (Tomlinson et al, 2004). In Arabidopsis, the root-specific expression of yeastderived apoplastic invertase had no detectable effect on the plant biomass as well (von Schweinichen and Bü ttner, 2005). In contrast, Arabidopsis plants expressing an apoplastic invertase of Chenopodium rubrum with the root-specific pyk10 promoter showed only slightly enhanced invertase activity and no changes in the soluble sugar contents, but there was an indirect influence on the whole plant development and an increased total plant biomass (von Schweinichen and Bü ttner, 2005).…”

Section: Discussionmentioning

confidence: 93%

“…In Arabidopsis, the root-specific expression of yeastderived apoplastic invertase had no detectable effect on the plant biomass as well (von Schweinichen and Bü ttner, 2005). In contrast, Arabidopsis plants expressing an apoplastic invertase of Chenopodium rubrum with the root-specific pyk10 promoter showed only slightly enhanced invertase activity and no changes in the soluble sugar contents, but there was an indirect influence on the whole plant development and an increased total plant biomass (von Schweinichen and Bü ttner, 2005). The authors observed a similar phenotype when the extracellular-located yeast invertase was driven under a meristem-specific promoter, which was observed in several other studies (e.g.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Schaarschmidt

González²,

Roitsch³

et al. 2007

PLANT PHYSIOLOGY

View full text Add to dashboard Cite

The mutualistic interaction in arbuscular mycorrhiza (AM) is characterized by an exchange of mineral nutrients and carbon. The major benefit of AM, which is the supply of phosphate to the plant, and the stimulation of mycorrhization by low phosphate fertilization has been well studied. However, less is known about the regulatory function of carbon availability on AM formation. Here the effect of enhanced levels of hexoses in the root, the main form of carbohydrate used by the fungus, on AM formation was analyzed. Modulation of the root carbohydrate status was performed by expressing genes encoding a yeast (Saccharomyces cerevisiae)-derived invertase, which was directed to different subcellular locations. Using tobacco (Nicotiana tabacum) alcTcwINV plants, the yeast invertase was induced in the whole root system or in root parts. Despite increased hexose levels in these roots, we did not detect any effect on the colonization with Glomus intraradices analyzed by assessment of fungal structures and the level of fungus-specific palmitvaccenic acid, indicative for the fungal carbon supply, or the plant phosphate content. Roots of Medicago truncatula, transformed to express genes encoding an apoplast-, cytosol-, or vacuolar-located yeastderived invertase, had increased hexose-to-sucrose ratios compared to b-glucuronidase-transformed roots. However, transformations with the invertase genes did not affect mycorrhization. These data suggest the carbohydrate supply in AM cannot be improved by root-specifically increased hexose levels, implying that under normal conditions sufficient carbon is available in mycorrhizal roots. In contrast, tobacco rolCTppa plants with defective phloem loading and tobacco pyk10TInvInh plants with decreased acid invertase activity in roots exhibited a diminished mycorrhization.Arbuscular mycorrhiza (AM) represents a widespread mutualistic association between soil-born fungi of the phylum Glomeromycota and most land plants. The AM interaction enables the plant to improve its supply of water and mineral nutrients, mainly phosphate. In return, the obligate biotrophic AM fungi are provided with carbon. In the Arum-type interaction, as analyzed here between Glomus sp. and tobacco (Nicotiana tabacum) or Medicago truncatula, the AM fungus colonizes the cortical cells by formation of intra-and intercellular hyphae and very characteristic haustorialike structures, the highly branched intracellular arbuscules. When the carbon supply is sufficient, lipid-rich vesicles are formed intercellularly within the cortex, as fungal storage organs. Intracellular fungal structures are separated from the plant cytoplasm by an extension of the plasma membrane, forming the periarbuscular membrane surrounding the arbuscule. The greatly increased surfaces of host and arbuscule plasma membranes offers optimized conditions for effective nutrient exchange via the established symbiotic interface (for review, see Gianinazzi-Pearson et al., 1996;Harrison, 1999).The exchange of phosphate via the periarbuscular interface and th...

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Schaarschmidt

González²,

Roitsch³

et al. 2007

PLANT PHYSIOLOGY

View full text Add to dashboard Cite

show abstract

“…The rol genes of Agrobacterium rhizogenes are known to affect plant hormone metabolism and/or sensitivity; therefore, these are suitable tools to modify plant development (van der Salm et al 1996), including apical dominance, enhanced rooting, accelerated flowering, and short life span. von Schweinichen and Büttner (2005) have reported that an increase in root mass and, consequently, in root surface area, allows the effective absorption of nutrients from the medium (soil), resulting in enhanced development. Although enhanced rooting in rol transformed plants positively affects plant architecture, whether or not this improves root profile to absorb more nutrients in water-deficient regions is yet to be proven.…”

Section: Discussionmentioning

confidence: 99%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Soybean (Glycine max L.) cultivar NARC-4 was transformed with constructs carrying rolA, rolB, or rolC genes, each under the control of the Cauliflower Mosaic Virus 70S promoter. Cotyledonary nodes of soybean seeds were infected with Agrobacterium tumefaciens strain LBA4404 carrying one of the three rol genes, along with nptII in the plasmid pLBR. The efficiency of the transformation varied slightly among the three constructs, with frequencies of 6, 7, and 5% for the rolA, rolB, and rolC genes, respectively, being observed. Southern blot analysis confirmed the integration of rol genes in the soybean genome with varying numbers of copies of the transgene. All transformed plants showed enhanced rooting, but the number of adventitious roots was higher for transformants carrying the rolB transgene. rolA and rolC transformants showed dwarf phenotypes, clustered branching, and variations in leaf morphology. Furthermore, these plants flowered within a short period of time and produced lower numbers of flowers. rolB transformants showed variations in phenotype, including dwarf to semidwarf and shrubby growth, abnormal stem growth, short internodes, variations in leaf morphology, and greenish to yellowish-green colored leaves. These plants also flowered early, but dwarf plants produced low numbers of flowers, while shrubby plants produced high numbers of flowers, but these were mostly infertile.

show abstract

“…In order to enhance sink strength, heterologous plant cell wall invertase (CrCIN1) was transformed into Arabidopsis thaliana. Under the control of a root-specific promoter, and the transgenic plants developed more secondary roots and subsequently, a higher shoot and whole plant biomass, furthermore flower in advance (Schweinichen and Büttner 2005). By expressing a CIN under a meristem-specific promoter, Arabidopsis thaliana increased CIN activity, caused accelerated flowering, elevation of the number of siliques which result from enhanced branching of the inflorescence and an increase in seed yield by nearly 30 % (Heyer et al 2004).…”

Section: Introductionmentioning

confidence: 98%

Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

et al. 2014

View full text Add to dashboard Cite

Cell wall invertase (CIN) plays an important role in carbohydrate partitioning and regulation of sinksource interaction. In this study, a full length cDNA encoding CIN gene was cloned by RT-PCR and RACE-PCR from sugarcane. The open reading frame of 1731 bp encodes a protein of 577 amino acids with a predicted molecular mass of 141.04 kDa and theoretical PI of 4.67. A size of 4,537 bp DNA encoding SoCIN1 was isolated, which contains seven exons and six introns. Using genome walking approach, a promoter sequence of 974 bp of SoCIN1 was isolated, and prediction with PlantCARE and PLACE software revealed that it has several key cis-regulatory elements known to be involved in various biotic and abiotic plant stresses. Relative expression in specific tissues was done using qRT-PCR, and the results demonstrated that SoCIN1 expression level was higher in the immature leaves and maturing leaves than the matured leaves and internodes at elongation stage and processing maturing stage. The conditions of 15 % PEG and 6°C could have a similar function for inducing the expression of SoCIN gene in leaves, while 15 % PEG, 100 mM NaCl and 6°C could induce the expression of SoCIN gene in roots.

show abstract

Expression of a Plant Cell Wall Invertase in Roots of Arabidopsis Leads to Early Flowering and an Increase in Whole Plant Biomass

Cited by 25 publications

References 51 publications

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Regulation of Arbuscular Mycorrhization by Carbon. The Symbiotic Interaction Cannot Be Improved by Increased Carbon Availability Accomplished by Root-Specifically Enhanced Invertase Activity

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.)

Contact Info

Product

Resources

About