Expression of a pea disease resistance response gene in the potato cultivar Shepody

Chang, Ming‐Mei; Chiang, Chin C.; Martin, Mark W.; Hadwiger, Lee A.

doi:10.1007/bf02849153

Cited by 33 publications

(14 citation statements)

References 23 publications

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“…Similar negative results have also been observed in the studies of pea PR-10.1 [26]. However, expression of the pea PR-10.1 gene in potato confers resistance to early dying disease [27]. In the recent studies, Chen et al [28] found RNAi-silenced kernels had a significant reduction in PR10 production and lower in aflatoxin production compared to no-RNAisilenced kernels in maize.…”

Section: Discussionsupporting

confidence: 64%

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Xie¹,

Wen²,

Liu³

et al. 2013

AJPS

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Peanut (Arachis hypogaea L.) is one of the most susceptible host crops to Aspergillus flavus invasion and subsequent aflatoxin contamination. In this report, a new member of PR10 family putative resistant gene (designated as ARAhPR10, No. EU661964.1) encoding a PR10 protein was isolated and characterized. Analysis of qRT-PCR showed that the expression of ARAhPR10 was induced by pre-harvested A. flavus infection, but no significant difference was observed between resistant genotype "GT-C20" and susceptible genotype "Yueyou 7". Seven transgenic peanut lines expressing the ARAhPR10 gene under the control of 35S promoter were obtained using the Agrobacterium tumefaciens-mediated method. Real time RT-PCR results showed that the expression level of the ARAhPR10 was significantly higher and the A. flavus infection and aflatoxin content were significantly lower in seeds of transgenic lines than that of the wild type. A significant negative correlation between ARAhPR10 expression at transcript level and seeds aflatoxin production was observed. Combining the previous results, it is suggested that ARAhPR10 expression play an important role in peanut host resistance to A. flavus infection and aflatoxin producing.

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Section: Discussionsupporting

confidence: 64%

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Xie¹,

Wen²,

Liu³

et al. 2013

AJPS

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“…Evaluating these results, other approaches revealed diverse qualitative outcomes concerning the disease severity upon infection with fungal or oomycete pathogens: Constitutive expression or overexpression of PR10s led to enhanced resistance levels (Chang et al 1993;Wang et al 1999), but there are also examples where PR-10 overexpression in transgenic plants did not reveal any alterations in resistance (Constable et al 1993). Moreover, many PRs and related proteins possess antimicrobial activities in vitro, but when expressed in transgenic plants, they reduce only a limited number of diseases (van Loon et al 2006).…”

Section: Discussionmentioning

confidence: 92%

Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Colditz

Niehaus

Krajinski

2007

Planta

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Recent studies on the root proteome of Medicago truncatula (Gaertn.) showed an induction of pathogenesis-related (PR) proteins of the class 10 after infection with the oomycete pathogen Aphanomyces euteiches (Drechs.). To get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using RNAi was carried out. Agrobacterium rhizogenes-mediated transformation of M. truncatula roots led to a knockdown of the Medicago PR10-1 gene in transgenic in vitro root cultures. Proteomic analyses of the MtPr10-1i root cultures showed that MtPr10-1 was eYciently knocked down in two MtPr10-1i lines. Moreover, Wve additional PR-10-type proteins annotated as abscisic acid responsive proteins (ABR17s) revealed also an almost complete silencing in these two lines. Inoculation of the root cultures with the oomycete root pathogen A. euteiches resulted in a clearly reduced colonization and thus in a suppressed infection development in MtPr10-1i roots as compared to that in roots of the transformation controls. In addition, MtPr10-1 silencing led to the induction of a new set of PR proteins after infection with A. euteiches including the de novo induction of two isoforms of thaumatin-like proteins (PR-5b), which were not detectable in A. euteiches-infected control roots. Thus, antagonistic induction of other PR proteins, which are normally repressed due to PR-10 expression, is supposed to cause an increased resistance of M. truncatula upon an A. euteiches in vitro infection. The results were also further conWrmed by detecting increased PR-5b induction levels in 2-D gels of a previously analyzed M. truncatula line (F83.005-9) exhibiting increased A. eìuteiches tolerance associated with reduced PR-10 induction levels.

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“…An increased understanding of these mechanisms may accelerate progress in developing novel control strategies for downy mildew diseases. Indeed, the DRR49 protein from pea has been used to increase potato tuber production in the presence of pathogeninfested soil (Chang et al 1993), and to increase levels of resistance to Leptosphaeria maculans in canola (Wang et al 1999b). It is therefore possible that the proteins identified in the current study may be of use in the development of novel plant lines with enhanced resistance to biotic and abiotic stresses.…”

Section: Discussionmentioning

confidence: 93%

Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae

et al. 2008

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A proteomic approach was used to identify host proteins altering in abundance during Peronospora viciae infection of a susceptible cultivar of pea (Pisum sativum cv. Livioletta). Proteins were extracted from fully developed pea leaflets at 4 days post-inoculation, before visible symptoms were apparent. Cytoplasmic proteins and membrane-and nucleic acid-associated proteins from infected and control leaves were examined using two-dimensional difference gel electrophoresis. The majority of proteins had a similar abundance in control and infected leaves; however, several proteins were altered in abundance and twelve were found to have increased significantly in the latter. These proteins were selected for either matrix-assisted laser desorption/ ionisation time-of-flight mass spectrometry or electrospray ionisation quadrupole time-of-flight tandem mass spectrometry analysis following trypsin digestion, with sequence identity being assigned to eight of the proteins. These included the ABR17 stress-response protein, the pathogen-induced PI176 protein, three photosynthetic proteins, a glycine-rich RNA binding protein and two glyceraldehyde 3-phosphate dehydrogenases (cytosolic and chloroplastic) which can be induced by a range of abiotic and biotic stresses in many plant species. The possible roles of these proteins in the response of the pea plant during P. viciae infection are discussed. This study represents the first proteomic analysis of downy mildew infection of pea leaves, and provides the basis for further work to elucidate molecular mechanisms of compatibility in P. viciae infections.

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Expression of a pea disease resistance response gene in the potato cultivar Shepody

Cited by 33 publications

References 23 publications

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae

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Expression of a pea disease resistance response gene in the potato cultivar Shepody

Cited by 33 publications

References 23 publications

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of &lt;i&gt;Aspergillus flavus&lt;/i&gt; Infection in Peanut Seeds

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of &lt;i&gt;Aspergillus flavus&lt;/i&gt; Infection in Peanut Seeds

Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches

Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae

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Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds

Overexpression of ARAhPR10, a Member of the PR10 Family, Decreases Levels of <i>Aspergillus flavus</i> Infection in Peanut Seeds