Expression of a novel complement C3 gene in the razor clam Sinonovacula constricta and its role in innate immune response and hemolysis

Peng, Maoxiao; Niu, Donghong; Chen, Zhiyi; Lan, Tianyi; Dong, Zhiguo; Tran, Thi-Nga; Li, Jiale

doi:10.1016/j.dci.2017.03.027

Cited by 30 publications

(31 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This proto-complement complex defined in a phylogenetic approach based on transcriptomic data from multiple invertebrates contains at least a C3 like-TEP, a serine protease C2/factor B and complement receptors ( 145 , 146 ), to induce an activated complement cascade on the pathogen surface leading to the non-self opsonization and elimination. Some molecules of this proto-complement have been characterized from several mollusk species like Complement factor C2/B from Bivalvia such as Ruditapes decussates ( 147 ) or Sinonovacula constricta ( 148 ), C3-like factors from mussels ( 149 ) or clams ( 150 ), but few studies led to the characterization of the whole system ( 145 , 151 ). While a diversity of C3-like proteins within the TEP superfamily has been reported ( 63 , 99 ) as well as a large repertoire of carbohydrate-binding proteins such as FREPs ( 46 , 152 ) and VIgL domain-containing proteins in B. glabrata ( 53 , 112 ), no complement factor C2/B-like protein has yet been identified.…”

Section: Discussionmentioning

confidence: 99%

New Insights Into Biomphalysin Gene Family Diversification in the Vector Snail Biomphalaria glabrata

et al. 2021

View full text Add to dashboard Cite

Aerolysins initially characterized as virulence factors in bacteria are increasingly found in massive genome and transcriptome sequencing data from metazoans. Horizontal gene transfer has been demonstrated as the main way of aerolysin-related toxins acquisition in metazoans. However, only few studies have focused on their potential biological functions in such organisms. Herein, we present an extensive characterization of a multigene family encoding aerolysins - named biomphalysin - in Biomphalaria glabrata snail, the intermediate host of the trematode Schistosoma mansoni. Our results highlight that duplication and domestication of an acquired bacterial toxin gene in the snail genome result in the acquisition of a novel and diversified toxin family. Twenty-three biomphalysin genes were identified. All are expressed and exhibited a tissue-specific expression pattern. An in silico structural analysis was performed to highlight the central role played by two distinct domains i) a large lobe involved in the lytic function of these snail toxins which constrained their evolution and ii) a small lobe which is structurally variable between biomphalysin toxins and that matched to various functional domains involved in moiety recognition of targets cells. A functional approach suggests that the repertoire of biomphalysins that bind to pathogens, depends on the type of pathogen encountered. These results underline a neo-and sub-functionalization of the biomphalysin toxins, which have the potential to increase the range of effectors in the snail’s immune arsenal.

show abstract

Section: Discussionmentioning

confidence: 99%

New Insights Into Biomphalysin Gene Family Diversification in the Vector Snail Biomphalaria glabrata

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In this experiment, the animals were exposed to the same conditions used in the long-term stress tests, except that the clams were not fed algae. At 0-h, 12-h, 24-h, and 48-h, ten JSCs from each group were used to measure oxygen consumption, then the hemolymph of JSCs was collected, as previously reported (Peng et al, 2017), to separate haemocytes and to measure metabolic and phagocytic activities.…”

Section: Methodsmentioning

confidence: 99%

Tolerance, Growth, and Physiological Responses of the Juvenile Razor Clam (Sinonovacula constricta) to Environmental Ca2+ and Mg2+ Concentrations

Peng

Liu

et al. 2019

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

To facilitate transplanting razor clam ( Sinonovacula constricta ) populations to inland saline-alkaline waters (ISWs), we evaluated the tolerance of juvenile S. constricta (JSC) to Ca 2+ and Mg 2+ concentrations, and determined the effects of these ions on JSC growth and physiological parameters. After 30 days stress, the tolerable ranges of JSC to Ca 2+ and Mg 2+ were determined to be 0.19 mmol⋅L -1 –19.46 mmol⋅L -1 and 0 mmol⋅L -1 –29.54 mmol⋅L -1 , respectively. The concentrations of Ca 2+ (less than 0.65 mmol⋅L -1 or more than 3.24 mmol⋅L -1 ) and Mg 2+ (less than 0.37 mmol⋅L -1 or more than 14.17 mmol⋅L -1 ) significantly inhibit JSC growth. Physiological enzyme activity no significant response when the concentrations range of Ca 2+ and Mg 2+ are 0.93 mmol⋅L -1 –6.49 mmol⋅L -1 and 0.37 mmol⋅L -1 –14.77 mmol⋅L -1 , respectively. For transplantation practice, these data indicate that only high concentrations of Ca 2+ (3.24–6.825 mmol⋅L -1 ) and Mg 2+ (14.77–33.69 mmol⋅L -1 ) in target inland saline-alkaline water had significantly impact on growth and physiological response. In addition, present study suggests that the increase in Ca 2+ and Mg 2+ ion concentrations caused by ocean acidification will not affect the survival, growth and physiology of S. constricta . Current research suggests that S. constricta can adapt to extreme changes in the marine environment (Ca 2+ and Mg 2+ ) and may be an excellent candidate for inland saline-alkaline water transplantation practice.

show abstract

“…Cq3 acts as a central innate immunity hub mediating opsonization and production of AMPs to eliminate pathogens and activating the complement cascade as the first line of immune defense (Ricklin et al, 2016;Volanakis, 1990). In marine bivalves such as the mussel Mytilus corruscus, the razor clam Sinonovacula constricta and oyster C. gigas, Cq3 is stimulated by bacterial infection (Vibrio and Micrococcus species), exposure to bacterial PAMPs or toxic insults (such as dissolved copper) (Chen et al, 2018;Dong et al, 2017;Peng et al, 2017Peng et al, , 2016Wang et al, 2017a). A highly expressed Cq3 system combined with the elevated expression of AMPs such as big defensin might contribute to a more rapid activation of the humoral immune defense and more effective pathogen killing in C. gigas compared with C. virginica.…”

Section: Mineral Sequestration and Cellular Transport Involve Metastamentioning

confidence: 99%

Potential trade-offs between biomineralization and immunity revealed by shell properties and gene expression profiles of two closely related Crassostrea species

Ivanina

Borah

Vogts

et al. 2018

Journal of Experimental Biology

View full text Add to dashboard Cite

Species of the Ostreidae family are key ecosystem engineers and many of them - including and - are commercially important aquaculture species. Despite similarities in their morphology and ecology, these two species differ in their ability to defend against pathogens, potentially reflecting species-specific differential specialization of hemocytes on immune defense versus biomineralization. To test this hypothesis, we investigated the expression levels of immune- and biomineralization-related genes as well as mineralogical and mechanical properties of the shells and the calcium sequestration ability of the hemocytes of and The expression of biomineralization-related genes was higher in than in in multiple tissues including the mantle edge and hemocytes, while the expression of immune genes was higher in the hemocytes of Hemocytes of contained more calcium (stored intracellularly as calcium carbonate mineral) compared with those of Analysis of the adult shells showed that the crystallinity of calcite was higher and the laths of the foliated layer of the shell were thicker in than in Mechanically, the shells of were stiffer, harder and stronger than those of Taken together, our results show that the species-specific differences in physiology (such as disease resistance and exoskeleton properties) are reflected at the cellular and molecular levels in the differential specialization of hemocytes on potentially competing functions (immunity and biomineralization) as well as different expression profiles of other tissues involved in biomineralization (such as the mantle edge).

show abstract

Expression of a novel complement C3 gene in the razor clam Sinonovacula constricta and its role in innate immune response and hemolysis

Cited by 30 publications

References 51 publications

New Insights Into Biomphalysin Gene Family Diversification in the Vector Snail Biomphalaria glabrata

New Insights Into Biomphalysin Gene Family Diversification in the Vector Snail Biomphalaria glabrata

Tolerance, Growth, and Physiological Responses of the Juvenile Razor Clam (Sinonovacula constricta) to Environmental Ca2+ and Mg2+ Concentrations

Potential trade-offs between biomineralization and immunity revealed by shell properties and gene expression profiles of two closely related Crassostrea species

Contact Info

Product

Resources

About