Expression of a Novel Chitinase by the Fungal Endophyte in Poa ampla

Li, Huaijun Michael; Sullivan, Ray; Moy, Melinda; Kobayashi, Donald Y.; Belanger, Faith C.

doi:10.2307/3762172

Cited by 18 publications

(10 citation statements)

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“…In particular, the production of amylase, cellulose, protease, and lipase by a large number of different endophytes in rice provide a wider spectrum of biological properties that can function at diverse pH and temperature ranges. A previous research study (Li et al, 2004) showed that several endophytic Neotyphodium species found in perennial rye grass secreted hydrolytic enzymes i.e. chitinase, proteases, glucanase, that are involved in the digestion of the insect cuticle and mycoparasitism.…”

Section: June 2015mentioning

confidence: 99%

“…These proteins are also reported to suppress plant pathogen activities directly by acting as pathogenicity related (PR) proteins. One function attributed to this is degradation of cell walls of the pathogenic fungi (Li et al, 2004).…”

Section: June 2015mentioning

confidence: 99%

“…chitinase, proteases, glucanase which are involved in the digestion of insect cuticle and mycoparasitism. Also they showed that Neoatyphodium-infected rye grasses have the ability to resist diseases and herbivoury infections (Li et al, 2004) June 2015…”

Section: June 2015mentioning

confidence: 99%

“…As endophytes are reported to enter the plants horizontally through seeds (Zabalgogeazcoa, 2008), seeds from both varieties were used to inoculate the above mentioned fungi. According to Li et al (2004), seeds lose viable endophytes during long-term storage and the resultant plants from long-term storage germinated seeds are endophyte-free. Therefore, seedlings/plants grown from LSSs are considered as endophyte-free and they proved to be so when tested by culturing on artificial media in the present study.…”

Section: June 2015mentioning

confidence: 99%

“…long storage seeds and recently harvested seeds from both varieties were used to inoculate the tested fungi. According to Li et al (2004), seeds that have lost the viable endophyte as a result of long-term storage, and resultant plants from long term storage germinated seeds are endophyte-free. Long storage seeds (LSSs) from both varieties, which have been stored at 1 o C for 20 years were selected as endophyte free controls for this study.…”

Section: June 2015mentioning

confidence: 99%

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Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

Atugala¹,

Deshappriya²

2015

J. Natn. Sci. Foundation Sri Lanka

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Traditional rice varieties, although low producers of yield, are more amenable to organic farming practices that cause less damage to the environment and human health. Improved growth and reduced disease incidence would produce higher yields, and endophytic fungi have proven to be effective in achieving these ends in wheat. With this in view, endophytic fungal assemblages of two traditional rice varieties of Sri Lanka i.e. Suwandel and Kaluheenati were evaluated for their effect on the growth and disease incidence of rice plants, because the endophytic mycoflora of these two traditional rice varieties and their effects have not been studied before. The most common and the highest occurring endophytes of both rice varieties i.e. Absidia and Cylindrocladium were tested to determine their effect on rice plant growth and the results showed that the plants inoculated with both fungal isolates showed significant increases (p ≤ 0.05) in plant height, fresh weight and dry weight. Twenty two endophytic fungal isolates common to both rice varieties were screened using dual culture assay for their ability to control the mycelial growth of Magnaporthe grisea, the causative agent of rice blast disease. All tested endophytes controlled pathogen growth by coiling the hyphae around the pathogen and forming clamps and loops. However, Absidia and Acremonium showed the highest growth inhibition of the pathogen (100 %) and showed an abundance of the above inhibitory structures. The effect of crude culture filtrates of thirteen endophytic fungi tested using the diffusion plate method indicated antagonistic activity against the rice pathogen. In planta tests were carried out to assess the effect of three endophytic fungi i.e. Acremonium, Absidia and Penicillium on infection by M. grisea. Disease symptoms were observed only on the leaves of the rice plants grown from Penicillium inoculated seedlings and on plants used as controls sprayed with a 1×10 7 spores/mL suspension of the pathogen. Pre-inoculation with Acremonium and Absidia were effective in preventing infection by the blast fungus. No disease symptoms were observed in plants pre-inoculated with endophytes and in plants used as controls sprayed with a 1×10 5 spores/mL suspension of the pathogen. Twenty one endophytic fungi common to both rice varieties were assessed by the culture plate method in this study.

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Section: June 2015mentioning

confidence: 99%

Section: June 2015mentioning

confidence: 99%

Section: June 2015mentioning

confidence: 99%

Section: June 2015mentioning

confidence: 99%

Section: June 2015mentioning

confidence: 99%

See 3 more Smart Citations

Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

Atugala¹,

Deshappriya²

2015

J. Natn. Sci. Foundation Sri Lanka

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Proteomic analysis of a filamentous fungal endophyte using EST datasets

et al. 2009

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Proteomic analysis of many species of fungi, particularly filamentous fungi, is difficult due to the lack of publicly available genome sequence data and the problems associated with cross-species comparisons. Furthermore, the detection of fungal proteins in biological systems where there are a greater number of proteins present from other eukaryote species provides additional challenges. We present an EST-based approach for identifying proteins from a fungal endophyte of temperate grasses and demonstrate that this method is well suited for fungi with minimal sequence data.

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Endophytic Fungi

Hallmann

Sikora

2011

Biological Control of Plant-Parasitic Nematodes:

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Expression of a Novel Chitinase by the Fungal Endophyte in Poa ampla

Cited by 18 publications

References 0 publications

Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

Effect of endophytic fungi on plant growth and blast disease incidence of two traditional rice varieties

Proteomic analysis of a filamentous fungal endophyte using EST datasets

Endophytic Fungi

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