Expression of A. niger US368 xylanase in E. coli: Purification, characterization and copper activation

Elgharbi, Fatma; Hlima, Hajer Ben; Farhat-Khemakhem, Ameny; Ayadi-Zouari, Dorra; Béjar, Samír; Hmida-Sayari, Aïda

doi:10.1016/j.ijbiomac.2014.12.005

Cited by 20 publications

(24 citation statements)

References 34 publications

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“…Xylanase was purified with SnakeSkin Pleated Dialysis Tubing. The recombinant enzyme was evaluated by SDS–PAGE analysis (Figure 4A) . The highly purified xylanase showed a single band on the Coomassie Brilliant Blue‐stained gel with a protein size of 23 kDa.…”

Section: Resultsmentioning

confidence: 99%

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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Xylanase is a member of an important family of enzymes that has been used in many biotechnological processes. However, the overall cost of enzyme production has been the main problem in the industrial application of enzymes. To obtain maximum xylanase production, statistical approaches based on the Plackett-Burman design and response surface methodology were employed. The results of the statistical analyses demonstrated that the optimal conditions for increased xylanase production were the following: inoculum size, 3.8%; maize meal, 4.5%; histidine, 0.6%; methanol, 1%; culture volume, 20%; bean pulp, 30 g L −1 ; and Tween-80, 0.8%; and pH 5.0. Verification of the optimization demonstrated that 3273 U mL −1 xylanase was observed under the optimal conditions in shake flask experiments. SDS-PAGE results showed that the size of xylanase protein was about 23 kDa. The results showed that the xylanase produced by fermentation came from Aspergillus Niger by MALDI-TOF-MS. The optimized medium resulted in 2.1-and 1.4-fold higher the activity of xylanase compared with the unoptimized medium (the main nutrients are maize meal and bean pulp) and laboratory medium (the main nutrients are yeast extract and peptone), respectively.The optimization of fermentation conditions is an effective means to reduce production cost and improve xylanase activity. K E Y W O R D Smethodology optimization, Plackett-Burman, xylanase Abbreviations: PBS, phosphate-buffered saline; RSM, response surface method.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Resultsmentioning

confidence: 99%

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Sun

Yan

Zhan

et al. 2020

Engineering in Life Sciences

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“…A final yield of 38.9% and purification fold of 36.97% was reported in this study. In another study involving A. niger xylanase cloned into E. coli , a three-step purification measure was adopted involving a Ni-NTA resin and FPLC Mono-Q chromatography to obtain a high yield of 67.5% [28]. The molecular mass of purified xylanase was determined by relative mobility of standard proteins on SDS-PAGE.…”

Section: Resultsmentioning

confidence: 99%

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Ravindran¹,

Williams²,

Jaiswal³

2019

Foods

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In this study, spent coffee waste (SCW) was used as the sole carbon source for xylanase production in solid state fermentation mode using Aspergillus niger. A Box–Behnken design was constructed using three parameters viz. temperature, initial moisture content, and log number of spores to determine the optimal fermentation condition. The best fermentation conditions for xylanase production were found to be incubation at 30 °C with an initial moisture content of 70% and using an inoculum of 6.5 × 106 spores/g of dry SCW. Furthermore, the design of experiments revealed that maintaining a medium composition of 0.2 g of yeast extract, 0.04 g of K2HPO4, and 0.03 g of MgSO4 increased xylanase production. Under optimised solid-state fermentation conditions an enzyme activity of 6495.6 IU/g of dry SCW was recorded, which was approximately 1.39-fold higher than that of control (4649 IU/g of dry SCW). The efficacy of the purified xylanase as a juice enrichment agent for strawberry, blueberry, and raspberry pulp was tested.

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“…Numerous patents have been granted globally for the processes that involved genetic engineering of microbial strains for xylanase production (Cheng et al, 2006;Dauvrin and Jonniaux, 2004). Several researchers have used E. coli as the expression host system for heterologous xylanase expression (Zafar et al, 2016;Elgharbi et al, 2015;Jun et al, 2009;Panbangred et al, 1985). The E. coli expressed xylanases are functionally active, though they lack Nglycosylation.…”

Section: Genetic Engineering Of Microorganisms For Maximization Of Xymentioning

confidence: 99%

Production and applications of xylanases – an overview

Malhotra

Chapadgaonkar

2018

bta

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Endo-1, 4-β-xylanase is an enzyme that depolymerises xylan, a major component of lignocellulose. The economic utilization of lignocellulosic biomass as a feedstock for the production of fuel and chemicals would represent a paradigm shift in industrial carbon utilization, allowing sustainable resources to replace the non-renewable energy resources like petroleum. However, lignocellulose is a highly recalcitrant material that is extremely difficult to depolymerize. Many microorganisms possess repertoires of enzymes which act synergistically to decompose various components of lignocellulosic biomass into simple sugars. Biomass-utilizing organisms are widely distributed within different species of archaea, bacteria, fungi, protists, plants, and animals. These organisms possess numerous lignocellulolytic enzymes that can hydrolyze cellulose, hemicelluloses, or lignin. The present paper gives a detailed account of strategies for production, purification and application of microbial xylanases. Conversion of lignocellulose into fermentable sugars and subsequent conversion into bioethanol has been discussed in the present paper.

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Expression of A. niger US368 xylanase in E. coli: Purification, characterization and copper activation

Cited by 20 publications

References 34 publications

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

The optimization of fermentation conditions for Pichia pastoris GS115 producing recombinant xylanase

Spent Coffee Waste as a Potential Media Component for Xylanase Production and Potential Application in Juice Enrichment

Production and applications of xylanases – an overview

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