Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

Rodrigues, Flávia Guimarães; Santos, Maíra Neves; Carvalho, T. X T De; Rocha, Bruno Coelho; Riehle, Michael A.; Pimenta, Paulo Filemon Paolucci; Abraham, Eappen G.; Jacobs-­Lorena, Marcelo; Brito, Cristiana Ferreira Alves de; Moreira, Luciano Andrade

doi:10.1111/j.1365-2583.2008.00791.x

Cited by 29 publications

(40 citation statements)

References 42 publications

(60 reference statements)

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“…These methods are hampered by insecticide resistance and the high maintenance costs required to sustain the measures. The development of genetically modified mosquitoes (GMMs) that are resistant to parasite infection (which aims to reduce and/or block Plasmodium transmission) is being undertaken (James et al 1999, Rodrigues et al 2008, Isaacs et al 2011, although the impact of GMMs in the field must be carefully evaluated prior to widespread use.…”

Section: Malaria Treatment and Drug-resistant Parasites -mentioning

confidence: 99%

New approaches in antimalarial drug discovery and development: a review

Aguiar

Rocha

Souza

et al. 2012

Mem. Inst. Oswaldo Cruz

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Malaria remains a major world health problem following the emergence and spread of Plasmodium falciparum that is resistant to the majority of antimalarial drugs. This problem has since been aggravated by a decreased sensitivity of Plasmodium vivax to chloroquine. This review discusses strategies for evaluating the antimalarial activity of new compounds in vitro and in animal models ranging from conventional tests to the latest high-throughput screening technologies. Antimalarial discovery approaches include the following: the discovery of antimalarials from natural sources, chemical modifications of existing antimalarials, the development of hybrid compounds, testing of commercially available drugs that have been approved for human use for other diseases and molecular modelling using virtual screening technology and docking. Using these approaches, thousands of new drugs with known molecular specificity and active against P. falciparum have been selected. The inhibition of haemozoin formation in vitro, an indirect test that does not require P. falciparum cultures, has been described and this test is believed to improve antimalarial drug discovery. Clinical trials conducted with new funds from international agencies and the participation of several industries committed to the eradication of malaria should accelerate the discovery of drugs that are as effective as artemisinin derivatives, thus providing new hope for the control of malaria

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Section: Malaria Treatment and Drug-resistant Parasites -mentioning

confidence: 99%

New approaches in antimalarial drug discovery and development: a review

Aguiar

Rocha

Souza

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…This phenomenon may be the result of a hitchhiking effect or insertional mutagenesis and not due to a negative effect of the transgenic protein, as although homozygous transgenic mosquitoes were replaced by wt, transgenic heterozygous mosquitoes persisted with wt. Rodrigues et al (2008) recently developed four transgenic lines of Ae. fluviatilis expressing the mutated phospholipase A2 enzyme (PLA2m) which was shown to significantly inhibit P. gallinaceum oocyst development.…”

Section: Mosquitoesmentioning

confidence: 99%

Safe and fit genetically modified insects for pest control: from lab to field applications

et al. 2010

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Insect transgenesis is continuously being improved to increase the efficacy of population suppression and replacement strategies directed to the control of insect species of economic and sanitary interest. An essential prerequisite for the success of both pest control applications is that the fitness of the transformant individuals is not impaired, so that, once released in the field, they can efficiently compete with or even out-compete their wild-type counterparts for matings in order to reduce the population size, or to spread desirable genes into the target population. Recent research has shown that the production of fit and competitive transformants can now be achieved and that transgenes may not necessarily confer a fitness cost. In this article we review the most recent published results of the fitness assessment of different transgenic insect lines and underline the necessity to fulfill key requirements of ecological safety. Fitness evaluation studies performed in field cages and medium/large-scale rearing will validate the present encouraging laboratory results, giving an indication of the performance of the transgenic insect genotype after release in pest control programmes.

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“…Several other researchers have followed this strategy and developed transgenic mosquitoes that cannot transmit their parasites. Bee venom phospholipases, synthetic antimalaria proteins like vida3, single chain antibodies (scFv) targeting malaria parasites, as well as an antimicrobial peptide cecropin A have been used as effectors and mosquitoes engineered to express them lack the ability to effectively transmit parasites [52][53][54][55][56]. RNAi-based resistance to dengue virus has also been engineered in Ae.…”

Section: Mosquitoesmentioning

confidence: 99%

Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies

Ogaugwu¹,

Durvasula²

2017

Biological Control of Pest and Vector Insects

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Insects are the most numerous of all animals and are found in almost every inhabitable place on earth. The order Diptera (true flies) contains many members that are notorious agricultural pests, nuisance or vectors of diseases. The list is long: mosquitoes, tsetse flies, screw worms, fruit flies, sand flies, blow flies, house flies, gall and biting midges, black flies, leaf miners, horse flies, and so on. Efforts to combat some of these pests and vectors have resulted in control measures such as the chemical, physical, and cultural control methods. These methods, though largely beneficial, have disadvantages and limitations, which sometimes seem to outweigh the problems initially sought to be controlled. The chemical method, for example, is not environment-friendly since it negatively affects many nontarget organisms and disrupts ecosystem balance. Development of insecticide resistance by pests/vectors is another concern. Molecular biotechnology has introduced vast arrays of novel ways to tackle pests and disease vectors, as well as improve the potency of existing control methods. This chapter looks at transgenic and paratransgenic biotechnologies and how they have been applied so far to develop and expand the arsenal against dipteran pests and disease vectors. Further, we discuss the advantages, disadvantages, and limitations of these technologies.

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Expression of a mutated phospholipase A₂ in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

Cited by 29 publications

References 42 publications

New approaches in antimalarial drug discovery and development: a review

New approaches in antimalarial drug discovery and development: a review

Safe and fit genetically modified insects for pest control: from lab to field applications

Developing the Arsenal Against Pest and Vector Dipterans: Inputs of Transgenic and Paratransgenic Biotechnologies

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