Expression of a Maize Ubiquitin Gene Promoter-<i>bar</i> Chimeric Gene in Transgenic Rice Plants

Toki, Seiichi; Takamatsu, Susumu; Nojiri, Chyuhei; Ooba, Shinya; Anzai, Hiroyuki; Iwata, Masaya; Christensen, Alan H.; Quail, Peter H.; Uchimiya, Hirofumi

doi:10.1104/pp.100.3.1503

Cited by 137 publications

(74 citation statements)

References 29 publications

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“…Thus, this strategy may make it unnecessary to use herbicide selection of T0 plants to eliminate untransformed escapes. Selection pressures of 5.0 and 10.0 mg/l of bialaphos have been reported in transformation of barley (Wan and Lemaux 1994), rice (Toki et al 1992), and maize (Brettschneider et al 1997). Our selection approach is similar to that reported by Wan and Lemaux (1994) for barley transformation.…”

Section: Molecular Analyses (Pcr Southern and Western) Of 33supporting

confidence: 65%

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

Zhang

Rybczyński

Langenberg³

et al. 2000

Plant Cell Reports

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Langenberg, W.G.; Mitra, Amitava; and French, Roy C., "An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration" (2000). Abstract A method for producing large numbers of transgenic wheat plants has been developed. With this approach, an average of 9.7% of immature embryo explants were transformed and generated multiple selffertile, independently transformed plants. No untransformed plants, or escapes, were regenerated. This transformation procedure uses morphogenic calli derived from scutellum tissue of immature embryos of Triticum aestivum cv. Bobwhite co-bombarded with separate plasmids carrying a selectable marker gene (bar) and a gene of interest, respectively. Transformed wheat calli with a vigorous growth phenotype were obtained by extended culture on media containing 5.0 mg/l bialaphos. These calli retained morphogenic potential and were competent for plant regeneration for as long as 11 months. The bar gene and the gene of interest were co-expressed in T0 progeny plants. This wheat transformation protocol may facilitate quantitative production of multiple transgenic plants and significantly reduce the cost and labor otherwise required for screening out untransformed escapes.

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Section: Molecular Analyses (Pcr Southern and Western) Of 33supporting

confidence: 65%

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

Zhang

Rybczyński

Langenberg³

et al. 2000

Plant Cell Reports

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“…The addition of cytokinin (BAP) and auxin (NAA) to regeneration medium enhanced the frequency of shoot regeneration from callus cultures [24,25]. Use of casein hydrolysate was found to be beneficial for generation of embryogenic calli in japonica rice [62,64,65]. Use of proline in the medium has also been reported to be effective for the initiation and maintenance of embryogenic calli [61,66].…”

Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.

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“…The combination of maize ubiquitin 1 promoter and the first intron of the coding region is capable of expressing foreign genes (26) at a high level in transgenic rice plants (27). DNA isolated from leaves of all the hygromycin-resistant plants was individually analyzed by PCR for the presence of the cryIAc gene.…”

Section: Fig 2 Ligand Blot Analysis Of 3-[(3-cholamidopropyl)dimethylmentioning

confidence: 99%

Transgenic elite indica rice plants expressing CryIAc ∂-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer ( Scirpophaga incertulas )

Nayak

Basu²,

Das³

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

218

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Generation of insect-resistant, transgenic crop plants by expression of the insecticidal crystal protein (ICP) gene of Bacillus thuringiensis (Bt) is a standard crop improvement approach. In such cases, adequate expression of the most appropriate ICP against the target insect pest of the crop species is desirable. It is also considered advantageous to generate Bt-transgenics with multiple toxin systems to control rapid development of pest resistance to the ICP. Larvae of yellow stem borer (YSB), Scirpophaga incertulas, a major lepidopteran insect pest of rice, cause massive losses of rice yield. Studies on insect feeding and on the binding properties of ICP to brush border membrane receptors in the midgut of YSB larvae revealed that cryIAb and cryIAc are two individually suitable candidate genes for developing YSB-resistant rice. Programs were undertaken to develop Bt-transgenic rice with these ICP genes independently in a single cultivar. A cryIAc gene was reconstructed and placed under control of the maize ubiquitin 1 promoter, along with the first intron of the maize ubiquitin 1 gene, and the nos terminator. The gene construct was delivered to embryogenic calli of IR64, an elite indica rice cultivar, using the particle bombardment method. Six highly expressive independent transgenic ICP lines were identified. Molecular analyses and insect-feeding assays of two such lines revealed that the transferred synthetic cryIAc gene was expressed stably in the T 2 generation of these lines and that the transgenic rice plants were highly toxic to YSB larvae and lessened the damage caused by their feeding.Technologies of plant cell biology and molecular biology have attracted much attention because they provide a powerful and novel means to supplement as well as complement the traditional methods of crop improvement. The transgenic approach of plant genetic engineering provides access to an unlimited gene pool for the transfer of desirable genes between any two species of interest, irrespective of their evolutionary or taxonomic relation. The successful generation of transgenic insectresistant crop plants by transferring the insecticidal crystal protein (ICP) gene of Bacillus thuringiensis (Bt; refs. 1-8), a Gram-positive, spore-forming bacterium, is one of the major breakthroughs in crop science that the new genetics has provided.indica rice is one of the most important food crops (9) of the world. There are half a dozen different species of lepidopteran insects whose larvae bore into the tissue of rice plants, and the resultant damage causes massive yield losses (10). Yellow stem borer (YSB), Scirpophaga incertulas, is most certainly the major contributor to these losses. The first-stage YSB larvae cut small holes in the stem of the paddy plant and enter the plant tissue, remaining there for the rest of their lives as larvae and pupae. The larvae feed voraciously inside the stem, providing themselves complete protection against chemical pesticides. There are no known resistance genes in rice (11) that can be rea...

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Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants

Cited by 137 publications

References 29 publications

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Transgenic elite indica rice plants expressing CryIAc ∂-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer ( Scirpophaga incertulas )

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