Expression of a kinase-dead form of CPK33 involved in florigen complex formation causes delayed flowering

Kawamoto, Nozomi; Endo, Motomu; Araki, Takashi

doi:10.1080/15592324.2015.1086856

Cited by 14 publications

(6 citation statements)

References 13 publications

(13 reference statements)

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“…We also observed a late flowering-time phenotype for the mutant cpk33 (Fig. 1 A and B), which is consistent with previously reported results [ 34 , 35 ]. In addition to CPK32, CPK23, CPK28 and CPK33 are also involved in the regulation of flowering time (Fig.…”

Section: Discussionsupporting

confidence: 93%

Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time

Chen

Yao

et al. 2021

National Science Review

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Appropriate flowering time is critical for the reproductive success of plant species. Emerging evidences indicate that calcium may play important roles in the regulation of flowering time. However, the underlying molecular mechanisms remain unclear. In this study, we demonstrate that CALCIUM-DEPENDENT PROTEIN KINASE 32 (CPK32) regulates flowering time by affecting the alternative polyadenylation of FLOWERING CONTROL LOCUS A (FCA) and altering the transcription of FLOWERING LOCUS C (FLC), a central repressor of flowering time. The knockdown of CPK32 results in an obvious late flowering phenotype and dramatically enhanced FLC transcription. CPK32 interacts with FCA, and phosphorylates the serine592 of FCA in a Ca2+-dependent manner. Moreover, the ratio of abundance of the FCA transcripts (FCA-D and FCA-P) changes significantly in the cpk32 mutant, which subsequently affects FLC expression and consequently regulates floral transition. The present evidences demonstrate that CPK32 modulates flowering time by regulating FCA alternative polyadenylation and consequent FLC expression.

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Section: Discussionsupporting

confidence: 93%

Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time

Chen

Yao

et al. 2021

National Science Review

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“…MtCDPK7, 17, 22, and MtCRK3, 6 transcripts level was lower than other genes. MtCDPK8, 16,18,19,22,23, and MtCRK1 transcript major observed in roots. MtCDPK17 and MtCRK2 were preferentially expressed in flower, while MtCDPK14, MtCDPK20, and MtCRK4 transcript were low in leaf.…”

Section: Spatial Expression Profiles Of Mtcdpk and Mtcrk Genesmentioning

confidence: 95%

Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula

Zhao

Liu

Kong

et al. 2021

IJMS

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Calcium-dependent protein kinase (CDPK or CPK) and CDPK-related kinase (CRK) play an important role in plant growth, development, and adaptation to environmental stresses. However, their gene families had been yet inadequately investigated in Medicago truncatula. In this study, six MtCRK genes were computationally identified, they were classified into five groups with MtCDPKs based on phylogenetic relationships. Six pairs of segmental duplications were observed in MtCDPK and MtCRK genes and the Ka/Ks ratio, an indicator of selection pressure, was below 0.310, indicating that these gene pairs underwent strong purifying selection. Cis-acting elements of morphogenesis, multiple hormone responses, and abiotic stresses were predicted in the promoter region. The spatial expression of MtCDPKs and MtCRKs displays diversity. The expression of MtCDPKs and MtCRKs could be regulated by various stresses. MtCDPK4, 14, 16, 22, and MtCRK6 harbor both N-myristoylation site and palmitoylation site and were anchored on plasma membrane, while MtCDPK7, 9, and 15 contain no or only one N-acylation site and were distributed in cytosol and nucleus, suggesting that the N-terminal acylation sites play a key role in subcellular localization of MtCDPKs and MtCRKs. In summary, comprehensive characterization of MtCDPKs and MtCRKs provide a subset of candidate genes for further functional analysis and genetic improvement against drought, cold, salt and biotic stress.

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“…Yet, single and double cpk6,33 mutants displayed only weak delayed flowering time compared to the fd mutant (Kawamoto et al ., 2015b), suggesting that additional CPKs are probably involved in this process. Accordingly, transgenic lines expressing a dominant negative version of AtCPK33 that can interact with FD without phosphorylating it exhibited a clear late‐flowering phenotype (Kawamoto et al ., 2015a).…”

Section: Biological Functions In Development and Metabolismmentioning

confidence: 99%

Properties and functions of calcium‐dependent protein kinases and their relatives in Arabidopsis thaliana

2019

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Summary Calcium is a ubiquitous second messenger that mediates plant responses to developmental and environmental cues. Calcium‐dependent protein kinases (CDPKs) are key actors of plant signaling that convey calcium signals into physiological responses by phosphorylating various substrates including ion channels, transcription factors and metabolic enzymes. This large diversity of targets confers pivotal roles of CDPKs in shoot and root development, pollen tube growth, stomatal movements, hormonal signaling, transcriptional reprogramming and stress tolerance. On the one hand, specificity in CDPK signaling is achieved by differential calcium sensitivities, expression patterns, subcellular localizations and substrates. On the other hand, CDPKs also target some common substrates to ensure key cellular processes indispensable for plant growth and survival in adverse environmental conditions. In addition, the CDPK‐related protein kinases (CRKs) might be closer to some CDPKs than previously anticipated and could contribute to calcium signaling despite their inability to bind calcium. This review highlights the regulatory properties of Arabidopsis CDPKs and CRKs that coordinate their multifaceted functions in development, immunity and abiotic stress responses.

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Expression of a kinase-dead form of CPK33 involved in florigen complex formation causes delayed flowering

Cited by 14 publications

References 13 publications

Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time

Calcium-dependent protein kinase CPK32 mediates calcium signaling in regulating Arabidopsis flowering time

Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula

Properties and functions of calcium‐dependent protein kinases and their relatives in Arabidopsis thaliana

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