Expression of a functional inducible nitric oxide synthase in hairy cell leukaemia and ESKOL cell line

Apoptosis induction by hypercross-linking of the surface antigen CD5 with anti-CD5 monoclonal antibodies in B cell chronic lymphocytic leukemia

“…As shown in Figure 5a, b and c, Bcl-2 and Mcl-1 protein expression suffered a significant decrease after CD5 hypercross-linking in B-CLL cells from patient group A (Bcl-2 MFIR 26.9 ± 7.7 control vs 12.5 ± 6.6 in CD5 hypercross-linked, P Ͻ 0.01, Mcl-1 MFIR 27.7 ± 7.4 control vs 11.5 ± 5.4, P Ͻ 0.01), while Bax protein expression was significantly increased (MFIR 14.9 ± 10.3 con- trol vs 29.5 ± 6.5, P Ͻ 0.01). Because a functional inducible nitric oxide synthase displaying anti-apoptotic activity was demonstrated in B-CLL and hairy cell leukemia, [25][26][27] we also examined iNOS expression after CD5 hypercross-linking in eight group A and 11 group B patients, and found a significant decrease of its levels in the B-CLL cells of group A patients (MFIR 10.8 ± 3.4 control vs 5.1 ± 2.0, P Ͻ 0.01), as shown in Figure 5d. Nothing significant happened with the expression of these apoptosis-related proteins in the cells from the 11 group B patients tested.…”

Mechanisms involved in the pro- and anti-apoptotic role of NO in human leukemia

“…73 Similar results were reported for ESKOL cells and for tumoral cells of HCL patients and it was shown that NO released played an anti-apoptotic role. 74,75 iNOS expression was also detected in seven out of eight human T cell leukemia virus type-I (HTLV-I)-infected cell lines (ATL-derived lines and in vitro transformed lines). Four out of nine ATL (adult T cell leukemia) patients of different subtypes (chronic or acute) also showed iNOS expression and the latter was found in all subtypes of ATL.…”

Nitric oxide induces the apoptosis of human BCR-ABL-positive myeloid leukemia cells: evidence for the chelation of intracellular iron

“…The expression of functional NOS-II in different human cell types including leukemia cells 11,13,29,30 investigated the role of NO as a member of anti-leukemia tools of macrophages. 3,31 The present study indicates that BCR-ABL + myeloid leukemia cells remained sensitive to the anti-proliferation property of exogenous NO.…”