Expression of a Cellular Oncogene During Liver Regeneration

Goyette, M; Petropoulos, Christos J.; Shank, Peter R.; Fausto, Nelson

doi:10.1126/science.6297003

Science

1983

DOI: 10.1126/science.6297003

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Expression of a Cellular Oncogene During Liver Regeneration

M Goyette

Christos J. Petropoulos

Peter R. Shank

et al.

Abstract: The number of transcripts of the cellular oncogene ras, which is homologous to the transforming gene of Harvey sarcoma virus, increases during liver regeneration in rats. The increase in these transcripts in liver polysomal polyadenylated RNA occurs at the time of activation of DNA synthesis during the regenerative process induced by partial hepatectomy or carbon tetrachloride injury. The number of ras transcripts returns to basal levels within 72 hours. These observations show that transcription of a cellular… Show more

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Cited by 258 publications

(90 citation statements)

References 22 publications

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“…Factors in the delayed response during G 1 might be triggers that are essential for the regeneration process. Some genes that contribute to the delayed response have already been identified, such as p53, c-ras, and various cyclins (13)(14)(15).…”

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confidence: 99%

Apolipoprotein A-V

Vliet¹,

Sammels²,

Leegwater³

et al. 2001

Journal of Biological Chemistry

248

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Liver regeneration in response to various forms of liver injury is a complex process, which ultimately results in restoration of the original liver mass and function. Because the underlying mechanisms that initiate this response are still incompletely defined, this study was aimed to identify novel factors. Liver genes that were up-regulated 6 h after 70% hepatectomy (PHx) in the rat were selected by cDNA subtractive hybridization. Besides known genes associated with cell proliferation, several novel genes were isolated. The novel gene that was most up-regulated was further studied. Its mRNA showed a liver-specific expression and encoded a protein comprising 367 amino acids. The mouse and human cDNA analogues were also isolated and appeared to be highly homologous. The human gene analogue was located at an apolipoprotein gene cluster on chromosome 11q23. The protein encoded by this gene had appreciable homology with apolipoproteins A-I and A-IV. Maximal expression of the gene in the rat liver and its gene product in rat plasma was observed 6 h after PHx. The protein was present in plasma fractions containing high density lipoprotein particles. Therefore, we have identified a novel apolipoprotein, designated apolipoprotein A-V, that is associated with an early phase of liver regeneration.

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mentioning

confidence: 99%

Apolipoprotein A-V

Vliet¹,

Sammels²,

Leegwater³

et al. 2001

Journal of Biological Chemistry

248

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“…Since a role in cell growth and differentiation has been proposed for c-myc (Goyette et al, 1983;Evan & Hancock, 1985), this study also evaluated staining with Mycl-6E10 in a range of gastric epithelial changes, some of which are considered to have malignant potential. The surface epithelium in histological sections of normal stomach, active superficial gastritis with foveolar hyperplasia, chronic superficial gastritis and atrophic gastritis both with and without intestinal metaplasia were examined using the streptavidin-biotin immunoperoxidase assay.…”

mentioning

confidence: 99%

Evaluation of p62c-myc in benign and malignant gastric epithelia

Allum

Newbold²,

Macdonald³

et al. 1987

Br J Cancer

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“…Sci. USA, in press), c-ras (13,27,28), and c-fos (18,29,38) and genes encoding p53 (48, 60), actin (13,29,46) serum and that adenovirus infection preferentially activates genes whose expression reaches a maximum in the late G1-S phase. …”

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confidence: 99%

Adenovirus type 2 activates cell cycle-dependent genes that are a subset of those activated by serum.

Liu¹,

Baserga²,

Mercer³

1985

Mol. Cell. Biol.

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We have studied a panel of 10 genes and cDNA sequences that are expressed in a cell cycle-dependent manner in different types of cells from different species and that are inducible by different mitogens. These include five sequences (c-myc, 4F1, 2F1, 2A9, and KC-1) that are preferentially expressed in the early part of the G, phase, three genes (ornithine decarboxylase, p53, and c-rasHa) preferentially expressed in middle or late G1, and two genes (thymidine kinase and histone H3) preferentially expressed in the S phase of the cell cycle. We have studied the expression of these genes in nonpermissive (tsAF8) and semipermissive (Swiss 3T3) cells infected with adenovirus type 2. Under the conditions of these experiments, adenovirus type 2 infection stimulates cellular DNA synthesis in both tsAF8 and 3T3 cells. However, four of the five early GI genes (c-myc, 4F1, KC-1, and 2A9) and one of the late G, genes (c-ras) are not induced by adenovirus infection, although they are strongly induced by serum. The other sequences (2F1, ornithine decarboxylase, p53, thymidine kinase, and histone H3) are activated by both adenovirus and serum. We conclude that the cell cycle-dependent genes activated by adenovirus 2 are a subset of the cell cycle-dependent genes activated by serum. The data suggest that the mechanisms by which serum and adenovirus induce cellular DNA synthesis are not identical.Cell cycle-dependent genes are operationally defined here as genes that are preferentially expressed in a specific phase of the cell cycle. A number of animal genes have already been identified whose expression is cell cycle dependent. These include histone genes (2, 22, 57), c-myc (13, 28, 35, 49; B. Calabretta, L. Kaczmarek, W. Mars, D. Ochoa, C. W. Gibson, R. R. Hirschhom, and R. Baserga, Proc. Natl. Acad. Sci. USA, in press), c-ras (13,27,28), and c-fos (18,29,38) and genes encoding p53 (48, 60), actin (13,29,46) serum and that adenovirus infection preferentially activates genes whose expression reaches a maximum in the late G1-S phase. MATERIALS AND METHODSCell line and culture conditions. tsAF8 cells are G1-specific, temperature-sensitive mutants originally isolated from BHK cells (12); these cells have been extensively characterized (3,12,25). Monolayer cultures are routinely maintained in Dulbecco modified Eagle medium containing 10% (vol/vol) donor calf serum at the PT of 34°C. To obtain quiescent (Go) cultures, tsAF8 cells are plated at 106 cells per 100-mm plate, grown to near confluence at 34°C, rinsed twice with prewarmed Hanks salt solution, fed with medium containing 0.5% (vol/vol) donor calf serum, and incubated for an additional 48 h at 34°C. Swiss 3T3 cells were cultured and made quiescent as previously described (47).Virus propagation, purification, and infection. Ad2 was grown in HeLa suspension cultures maintained in minimum essential modified suspension medium (Flow Laboratories, Rockville, Md.) with 5% fetal calf serum. Virus was purified as described previously (63)

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Expression of a Cellular Oncogene During Liver Regeneration

Cited by 258 publications

References 22 publications

Apolipoprotein A-V

Apolipoprotein A-V

Evaluation of p62c-myc in benign and malignant gastric epithelia

Adenovirus type 2 activates cell cycle-dependent genes that are a subset of those activated by serum.

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