Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid

Lyon, B. R.; Llewellyn, Danny J.; Huppatz, John L.; Dennis, Elizabeth S.; Peacock, W. James

doi:10.1007/bf00027313

Cited by 60 publications

(18 citation statements)

References 22 publications

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“…Many herbicide-degrading or -detoxifying enzymes, such as the phosphinothricin acetyltransferase Bar (38), bromoxynil-specific nitrilase Bxn (29), 2,4-dichlorophenoxyacetate monooxygenase TfdA (23), and atrazine chlorohydrolase AtzA (36), have been successfully used to construct herbicide-resistant crops. AmpA is a simple homodimer and is able to hydrolyze a variety of amide herbicides to herbicidally inactive products with high hydrolysis efficiency and does not need any cofactor for its activity.…”

Section: Discussionmentioning

confidence: 99%

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

Zhang

Yin

Hang

et al. 2012

Appl Environ Microbiol

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bThe bacterial isolate Paracoccus sp. strain FLN-7 hydrolyzes amide pesticides such as diflubenzuron, propanil, chlorpropham, and dimethoate through amide bond cleavage. A gene, ampA, encoding a novel arylamidase that catalyzes the amide bond cleavage in the amide pesticides was cloned from the strain. ampA contains a 1,395-bp open reading frame that encodes a 465-aminoacid protein. AmpA was expressed in Escherichia coli BL21 and homogenously purified using Ni-nitrilotriacetic acid affinity chromatography. AmpA is a homodimer with an isoelectric point of 5.4. AmpA displays maximum enzymatic activity at 40°C and a pH of between 7.5 and 8.0, and it is very stable at pHs ranging from 5.5 to 10.0 and at temperatures up to 50°C. AmpA efficiently hydrolyzes a variety of secondary amine compounds such as propanil, 4-acetaminophenol, propham, chlorpropham, dimethoate, and omethoate. The most suitable substrate is propanil, with K m and k cat values of 29.5 M and 49.2 s ؊1 , respectively. The benzoylurea insecticides (diflubenzuron and hexaflumuron) are also hydrolyzed but at low efficiencies. No cofactor is needed for the hydrolysis activity. AmpA shares low identities with reported arylamidases (less than 23%), forms a distinct lineage from closely related arylamidases in the phylogenetic tree, and has different biochemical characteristics and catalytic kinetics with related arylamidases. The results in the present study suggest that AmpA is a good candidate for the study of the mechanism for amide pesticide hydrolysis, genetic engineering of amide herbicide-resistant crops, and bioremediation of amide pesticide-contaminated environments.A variety of amide compounds are used as pesticides to control insects, pathogens, and weeds in agriculture. These compounds include benzoylurea insecticides (hexaflumuron, diflubenzuron, etc.), organophosphate insecticides with an amide group (dimethoate, omethoate, etc.), carbamate insecticides or herbicides (carbofuran, carbaryl, propham, chlorpropham, etc.), benzimidazole fungicides (carbendazim, benomyl, etc.), acetanilide herbicides (acetochlor, butachlor, propanil, etc.), substituted phenylurea herbicides (diuron, linuron, etc.), and sulfonylurea herbicides (chlorsulfuron, metsulfuron-methyl, etc.) (33). However, the widespread use of amide pesticides has resulted in the contamination of the environment and agricultural products. Moreover, many amide pesticides are hazardous to human health and may damage crops when used improperly (7,8,33). Thus, the removal of amide pesticides from the environment and agricultural products is of paramount importance.Microorganisms play a significant role in the degradation or detoxification of amide pesticides in the environment. Bacterial strains that are able to degrade amide pesticides (carbofuran, carbaryl, acetochlor, carbendazim, dimethoate, etc.) have been isolated, and their microbial metabolic pathways have also been elucidated (12,22,25,32,43). To date, the sequence information for the following amide pesticide-hydrolyzing enzymes is...

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Section: Discussionmentioning

confidence: 99%

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

Zhang

Yin

Hang

et al. 2012

Appl Environ Microbiol

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“…Por meio de técnicas de transgenia, foi possível realizar a transferência desse gene para espécies cultivadas, tornando-as tolerantes ao herbicida 2,4-D (Lyon et al, 1989;Lyon et al, 1993;Bisht et al, 2004;Mulwa et al, 2007). Por exemplo, a inserção do gene tfdA em plantas de Nicotiana tabacum (mediada por Agrobacterium tumefaciens) permitiu a sobrevivência de plantas após a utilização do 2,4-D (Lyon et al, 1989). Plantas de Gossypium hirsutum transformadas com esse gene também apresentaram tolerância ao herbicida (Lyon et al, 1993).…”

Section: Descoberta Do Gene E Da Enzima De Detoxificação Do 24-dunclassified

O desenvolvimento de culturas tolerantes ao herbicida diclorofenoxiacetato: revisão de literatura

Queiroz

Vidal

2014

Planta daninha

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RESUMO -O composto diclorofenoxiacetato (2,4-D) foi o primeiro herbicida orgânico, sistêmico, seletivo e de aplicação em pós-emergência desenvolvido no mundo. Juntamente com a revolução verde, ele contribuiu para elevar a produção dos cereais nas décadas posteriores a 1950. Esse produto é uma auxina sintética que pode ser utilizada como regulador do crescimento vegetal ou, ainda, como herbicida para o controle de espécies daninhas dicotiledôneas. Várias espécies infestantes dicotiledôneas que apresentam dificuldade de controle com outros herbicidas são suscetíveis ao 2,4-D. Contudo, a utilização desse herbicida fica restrita pela falta de seletividade em algumas culturas agrícolas. Nas últimas décadas, a descoberta de genes relacionados à tolerância ao 2,4-D em bactérias encontradas no solo e a sua transferência para culturas possibilitaram o desenvolvimento de linhagens tolerantes ao produto. Os objetivos desta revisão de literatura foram apresentar os genes e a atividade das enzimas responsáveis pela tolerância ao herbicida 2,4-D; ilustrar os mecanismos envolvidos na seletividade ao 2,4-D e a outros herbicidas; e equacionar algumas implicações para o manejo de plantas daninhas. ABSTRACT -The compound dichlorophenoxyacetate (2,4-D) was the first organic, systemic, selective herbicide, and for post-emergence application to be developed in the world. Along with the green revolution, this herbicide has helped to increase the cereal production on the decades after 1950. This product is a synthetic auxin that can be used as a plant growth regulator or as an herbicide for

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“…The tfdA genefrom the Gram-negative bacterium Ralstonia eutrophus strain JMP134 encodes the enzyme 2,4-D monooxygenase, involved in the conversion of 2,4-D to the non-active enzyme 3-oxoadipate (Streber et al 1987). Lyon et al (1989) cloned the gene tfdA behind a CaMV 35S promoter in a binary vector and introduced it into tobacco plants by Agrobacterium-mediated transformation. Through leaf discs and seed germination assays, the researchers verified that tfdA transgenic tobacco plants expressing 2,4-D monooxygenase showed increased tolerance to exogenous application of 2,4-D compared to wild-type plants.…”

Section: 4-dmentioning

confidence: 99%

Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects

Jube¹,

Borthakur²

2007

Electron. J. Biotechnol.

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Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid

Cited by 60 publications

References 22 publications

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

O desenvolvimento de culturas tolerantes ao herbicida diclorofenoxiacetato: revisão de literatura

Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects

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