Expression of 3b-Hydroxysteroid dehydrogenase and P450 side chain cleavage enzyme in the human uterine endometrium

Rhee, Hee Sub; Oh, Seon Hee; Ko, Bum Joo; Han, Dong; Jeon, Byung Hun; Park, Hyun; Moon, Hyung Bae; Kim, Won Sin

doi:10.1038/emm.2003.22

Cited by 27 publications

(26 citation statements)

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“…Although morphological and functional changes in the endometrial glands throughout the oestrous cycle and pregnancy are induced by steroids secreted by the ovary and/or placenta, localisation of 3b-HSD to the endometrium in women (Seki et al 1987, Tang et al 1993, Rhee et al 2003, monkeys (Martel et al 1994) and rats (Zaho et al 1991) has led to the proposal that endometrial 3b-HSD is involved in the local metabolism of circulating steroids. If the endometrial glands in the pregnant giraffe are indeed synthesising steroids, it is reasonable to assume that these may act in an autocrine manner to stimulate the hypertrophy and hyperplasia noted in the epithelium of the endometrial glands clustered around the base of the placentomes.…”

Section: Discussionmentioning

confidence: 99%

Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis)

Wilsher¹,

Stansfield

Greenwood

et al. 2013

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Gross, histological and immunocytochemical examinations carried out on maternal and fetal reproductive tissues from two pregnant giraffes at an estimated 8 and 13.5 months of gestation (termZ15 months) revealed a typically ruminant macrocotyledonary placenta with binucleate trophoblast cells scattered sparsely in the placentome where they stained intensely with a prolactin antiserum. Binucleate cells were present in greater numbers in the intercotyledonary allantochorion where they did not stain for prolactin whereas the uninucleate trophoblast still did. A single large corpus luteum of pregnancy and several small luteinised follicles were present in the maternal ovaries while the fetal ovaries at 13.5 months gestation showed an assortment of enlarging antral follicles and partially and completely lutenised follicles, the granulosa and luteal cells of which stained positively for 3b-hydroxysteroid dehydrogenase (3b-HSD), 17,20 lyase, prolactin, progesterone receptor and androgen receptor, but negatively for aromatase. The uninucleate trophoblast of the placentome and intercotyledonary allantochorion, the epithelium of the maternal endometrial glands, the seminiferous epithelium in the fetal testis at 8 months of gestation and the zonae fasciculata and reticularis of the fetal adrenal at 13.5 months also stained positively for 3b-HSD and negatively for aromatase. Endocrinologically, it appears that the giraffe placenta is more similar to that of the sheep than the cow with a placental lactogen as the likely driver of the considerable degree of luteinisation seen in both the maternal and the fetal ovaries.

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Section: Discussionmentioning

confidence: 99%

Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis)

Wilsher¹,

Stansfield

Greenwood

et al. 2013

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“…Rhee and coworkers reported the expression of HSD3B mRNA in the human endometrial tissue homogenates and described 3BHSD protein expression in total endometrial tissue lysates (Rhee et al 2003). In the same study, glandular epithelial cells immunopositive for 3BHSD were detected in proliferative and secretory phase endometrial samples (Rhee et al 2003). Immunoexpression was reported to be strongest in the first-trimester decidua and the decidua of ectopic pregnancy consistent with a potential role for this enzyme in decidual steroidogenesis and regulation of early pregnancy.…”

Section: Androgen Biosynthesis and Expression Of Steroidmetabolising mentioning

confidence: 91%

“…Following induction of aromatase expression in decidualised ESCs (Gibson et al 2013), local oestrogens regulate immune-mediated vascular remodelling by altering the function of uNK cells in early pregnancy (Gibson et al 2015). Rhee and coworkers reported the expression of HSD3B mRNA in the human endometrial tissue homogenates and described 3BHSD protein expression in total endometrial tissue lysates (Rhee et al 2003). In the same study, glandular epithelial cells immunopositive for 3BHSD were detected in proliferative and secretory phase endometrial samples (Rhee et al 2003).…”

Section: Androgen Biosynthesis and Expression Of Steroidmetabolising mentioning

confidence: 97%

“…Expression of the key steroidogenic enzymes, steroidogenic acute regulatory protein (STAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1) and cytochrome P450 family 17 subfamily A member 1 (CYP17A1) that convert cholesterol to the common sex steroid hormone precursor DHEA have been reported in the endometrium (Aghajanova et al 2009, Chen et al 2011, Huhtinen et al 2014. STAR mRNA is reported to be expressed at low levels in ESCs but not in epithelial cells (Hukkanen et al 1998, Rhee et al 2003, Aghajanova et al 2009). However, STAR protein levels in total endometrial tissue homogenates detected by western blotting analysis were reported to be low to undetectable (Tsai et al 2001, Aghajanova et al 2009, Attar et al 2009), and to our knowledge, there are no studies reporting CYP11A1 or CYP17A1 protein expression or enzymatic activity in the endometrium.…”

Section: Androgen Biosynthesis and Expression Of Steroidmetabolising mentioning

confidence: 99%

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Regulation of androgen action during establishment of pregnancy

Gibson¹,

Simitsidellis²,

Saunders³

2016

Journal of Molecular Endocrinology

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During the establishment of pregnancy, the ovarian-derived hormones progesterone and oestradiol regulate remodelling of the endometrium to promote an environment that is able to support and maintain a successful pregnancy. Decidualisation is characterised by differentiation of endometrial stromal cells that secrete growth factors and cytokines that regulate vascular remodelling and immune cell influx. This differentiation process is critical for reproduction, and inadequate decidualisation is implicated in the aetiology of pregnancy disorders such as foetal growth restriction and preeclampsia. In contrast to progesterone and oestradiol, the role of androgens in regulating endometrial function is poorly understood. Androgen receptors are expressed in the endometrium, and androgens are reported to regulate both the transcriptome and the secretome of endometrial stromal cells. In androgen-target tissues, circulating precursors are activated to mediate local effects, and recent studies report that steroid concentrations detected in endometrial tissue are distinct to those detected in the peripheral circulation. New evidence suggests that decidualisation results in dynamic changes in the expression of androgen biosynthetic enzymes, highlighting a role for pre-receptor regulation of androgen action during the establishment of pregnancy. These results suggest that such enzymes could be future therapeutic targets for the treatment of infertility associated with endometrial dysfunction. In conclusion, these data support the hypothesis that androgens play a beneficial role in regulating the establishment and maintenance of pregnancy. Future studies should be focussed on investigating the safety and efficacy of androgen supplementation with the potential for utilisation of novel therapeutics, such as selective androgen receptor modulators, to improve reproductive outcomes in women.

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“…In the last step of progesterone biosynthesis, 3b-hydroxysteroid dehydrogenase/ ketosteroid isomerases (HSD3B) types 1 and 2 catalyze isomerization at the D5 position and oxidation of the hydroxy group at position C3, and in this way they convert pregnenolone to progesterone ( Fig. 1) [13].…”

Section: Introductionmentioning

confidence: 99%

Altered expression of genes involved in progesterone biosynthesis, metabolism and action in endometrial cancer

Sinreih

Hevir

Rižner

2013

Chemico-Biological Interactions

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Expression of 3b-Hydroxysteroid dehydrogenase and P450 side chain cleavage enzyme in the human uterine endometrium

Cited by 27 publications

References 0 publications

Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis)

Ovarian and placental morphology and endocrine functions in the pregnant giraffe (Giraffa camelopardalis)

Regulation of androgen action during establishment of pregnancy

Altered expression of genes involved in progesterone biosynthesis, metabolism and action in endometrial cancer

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