Expression of 3 beta-hydroxysteroid dehydrogenase/isomerase in the female rat pituitary

Vidal, Sergio; Román, Albina; Moya, Lucas; Kovács, K.

doi:10.1677/joe.0.1660095

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…Steroid synthesis is a finely regulated, complex mechanism that can only occur in places where functional enzymes exist. The enzyme 3β‐hydroxysteroid dehydrogenase (3β‐HSD), which catalyses the conversion of pregnenolone into progesterone (P 4 ), is expressed not only in classical steroid‐producing glands, such as the ovary, the adrenal gland and the placenta, but also in several brain areas, including the hypothalamus and the pituitary gland, amongst others . Neurosteroids in the CNS regulate different brain areas involved in the modulation of mood, behaviour and cognition.…”

Section: Introductionmentioning

confidence: 99%

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

Cáceres

Orozco

Cabrera

et al. 2020

J Neuroendocrinology

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The present study aimed to determine whether an i.c.v. administration of allopregnanolone (ALLO) rapidly modifies the hypothalamic and ovarian 3β‐hydroxysteroid dehydrogenase (3β‐HSD) enzymatic activity and gene expression in in vivo and ex vivo systems in pro‐oestrus (PE) and dioestrus I (DI) rats. Animals were injected with vehicle, ALLO, bicuculline or bicuculline plus ALLO and were then killed. In the in vivo experiment, the hypothalamus, ovaries and serum were extracted and analysed. In the ex vivo experiment, the superior mesenteric ganglion ‐ ovarian nerve plexus ‐ ovary system was extracted and incubated during 120 minutes at 37 ºC. The serum and ovarian compartment fluids were used to determine progesterone by radioimmunoanalysis. In the in vivo experiments, ALLO caused a decrease in hypothalamic and ovarian 3β‐HSD enzymatic activity during PE. During DI, ALLO increased hypothalamic and ovarian 3β‐HSD activity and gene expression. The ovarian 3β‐HSD activity increased in both stages in the ex vivo system; gene expression increased only during DI. ALLO induced an increase in serum progesterone only in D1 and in the ovarian incubation liquids in both stages. All findings were reversed by an injection of bicuculline before ALLO. Ovarian steroidogenic changes could be attributed to signals coming from ganglion neurones, which are affected by the acute central neurosteroid stimulation. The i.c.v. administration of ALLO via the GABAergic system altered 3β‐HSD activity and gene expression, modulating the neuroendocrine axis. The present study reveals the action that ALLO exerts on the GABAA receptor in both the central and peripheral nervous system and its relationship with hormonal variations. ALLO is involved in the “fine tuning” of neurosecretory functions as a potent modulator of reproductive processes in female rats.

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Section: Introductionmentioning

confidence: 99%

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

Cáceres

Orozco

Cabrera

et al. 2020

J Neuroendocrinology

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“…Pg can also be metabolized by 20α-hydroxysteroid dehydrogenase (20α-HSD) (Clementi et al 2004). These enzymes are present in different regions, including the hypothalamus (Guennoun et al 1995, Vidal et al 2000 and the ovary (Vega Orozco et al 2012). Micevych and Sinchak (2008) mentioned that neurosteroids are not isolated from peripheral steroid sources.…”

Section: Introductionmentioning

confidence: 99%

A single dose of allopregnanolone affects rat ovarian morphology and steroidogenesis

Pelegrina¹,

Cáceres²,

Giuliani³

et al. 2017

Reproduction

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Allopregnanolone, a progesterone metabolite, is one of the best characterized neurosteroids. In a dose that mimics serum levels during stress, allopregnanolone inhibits sexual receptivity and ovulation and induces a decrease in luteinizing hormone levels. The aim of this work was to examine the effect of an intracerebroventricular administration of allopregnanolone on ovarian morphophysiology, serum and tissue levels of progesterone and estrogen, and enzymatic activity of 3β-hydroxysteroid dehydrogenase, 20α-hydroxysteroid dehydrogenase and 3α-hydroxysteroid oxido-reductase in the ovary and in the medial basal hypothalamus on the morning of estrus. Ovarian morphology was analyzed under light microscopy. The hormone assays were performed by radioimmunoassay. The enzymatic activities were measured by spectrophotometric analysis. The morphometric analysis revealed that, in allopregnanolone-treated animals, the number of secondary and Graafian follicles was decreased while that of atretic follicles and cysts was significantly increased. Some cysts showed luteinized unruptured follicles. There were no differences in the number of tertiary follicles or corpora lutea in comparison with the corresponding control groups. In allopregnanolone-treated animals, progesterone serum levels were increased, while ovarian progesterone levels were decreased. Moreover, 3β-HSD and 3α-HSOR enzymatic activities were increased in the medial basal hypothalamus while ovarian levels were decreased. The enzyme 20α-hydroxysteroid dehydrogenase showed the opposite profile. The results of this study showed that allopregnanolone interferes on ovarian steroidogenesis and ovarian morphophysiology in rats, providing a clear evidence for the role of this neurosteroid in the control of reproductive function under stress situations.

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Reproductive performance is associated with seasonal plasma reproductive hormone levels, steroidogenic enzymes and sex hormone receptor expression levels in cultured Asian yellow pond turtles (Mauremys mutica)

Wang

Liu

et al. 2021

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Expression of 3 beta-hydroxysteroid dehydrogenase/isomerase in the female rat pituitary

Cited by 6 publications

References 42 publications

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

A single dose of allopregnanolone affects rat ovarian morphology and steroidogenesis

Reproductive performance is associated with seasonal plasma reproductive hormone levels, steroidogenic enzymes and sex hormone receptor expression levels in cultured Asian yellow pond turtles (Mauremys mutica)

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