Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans

Thuy, Mai Le; Kharel, Madan K.; Lamichhane, Rajan; Lee, Hei Chan; Suh, Joo‐Won; Liou, Kwangkyoung; Sohng, Jae Kyung

doi:10.1007/s10529-005-2222-y

Cited by 15 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…91 In addition to BtrC, a number of DOI synthases have recently been isolated from several aminoglycoside biosynthetic gene clusters, including the ribostamycin, neomycin, kanamycin, tobramycin, and gentamicin gene clusters. 76,78,79,82, 92 Comparison of the active site residues of the crystallized DHQ synthase from Aspergillus nidulans with the new class of DOI synthases shows several key differences within the active site, including the conversion of R264 and N268 to a glycine residue and an acidic aspartate, respectively. These alterations are highly conserved within all of the available sequences for DOI synthases creating a novel sequence signature for this subfamily.…”

Section: Discovery Of 2-deoxy-scyllo-inosose Synthasesmentioning

confidence: 99%

Biosynthesis of aminocyclitol-aminoglycoside antibiotics and related compounds

Flatt,

Mahmud

2007

Nat. Prod. Rep.

124

120

View full text Add to dashboard Cite

show abstract

Section: Discovery Of 2-deoxy-scyllo-inosose Synthasesmentioning

confidence: 99%

Biosynthesis of aminocyclitol-aminoglycoside antibiotics and related compounds

Flatt,

Mahmud

2007

Nat. Prod. Rep.

124

120

View full text Add to dashboard Cite

show abstract

“…caesius was grown in APM medium (60 g glucose, 8 g yeast extract, 20 g malt extract, 2 g NaCl, 15 g MOPS (3-(N-morpholino) propanesulfonic acid) and 10 ml l )1 distilled water). For the transformation, recombinant DNA was constructed on pIBR25 under the control of the ermE* promoter, which leads the expression in Streptomyces (Thuy et al 2005). A demethylation host, E. coli ET12567, was used to obtain demethylated DNA.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Identification of the duplicated genes for S ‐adenosyl‐ l ‐methionine synthetase ( metK1‐sp and metK2‐sp ) in Streptomyces peucetius var. caesius ATCC 27952

Niraula

Liou

et al. 2010

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To characterize the function of both metK1‐sp (sp1190) and metK2‐sp (sp1566) in vitro and in vivo, and to study the regulation of doxorubicin production by overexpressing the metK. Methods and Results: We cloned two orfs into pET32a(+) respectively, and the formation of S‐Adenosyl‐l‐methionine was clearly observed in the in vitro enzyme assays as functional MetKs. Reverse transcriptase polymerase chain reaction (PCR) analysis indicated that the transcripts for the metK1‐sp were repressed as Streptomyces cells entered the decline phase, whereas that of the metK2‐sp was induced, suggesting that these MetK proteins may be important for the growth and the regulation of secondary metabolites during the stationary growth phase, whether considered together or separately. Furthermore, we found that the introduction of high‐copy‐number plasmids containing the metK1‐sp and metK2‐sp resulted in 2·1‐ and 1·4‐fold greater levels of doxorubicin production than the control transformants containing only the vector, respectively. We also attempted to disrupt the metK‐sp and found that doxorubicin production from the metK1‐sp‐deleted mutant (Streptomyces peucetius/pNN1) was reduced when compared to the parent strain (S. peucetius var. caesius ATCC 27952). Conclusions: The results of this study indicated that two metK are differentially expressed during cell growth, and that the expressions of the two metK genes are differentially regulated under the same conditions. Significance and Impact of the Study: Streptomyces peucetius var. caesius contains two genes, metK1‐sp and metK2‐sp, which encode functional S‐adenosyl‐l‐methionine synthetase (MetK). The degree of homology (90% identity) found between the two genes shows that metK1‐sp and metK2‐sp are duplicated genes. Although there is currently no evidence for the relationship of the duplicated metK genes involved in the regulation of doxorubicin production, metK1‐sp and metK2‐sp may play a role in controlling the stimulation of antibiotic production during secondary metabolism.

show abstract

Machine‐Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters

Lee,

Choe,

Palsson

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Streptomyces produces diverse secondary metabolites of biopharmaceutical importance, yet the rate of biosynthesis of these metabolites is often hampered by complex transcriptional regulation. Therefore, a fundamental understanding of transcriptional regulation in Streptomyces is key to fully harness its genetic potential. Here, independent component analysis (ICA) of 454 high‐quality gene expression profiles of the model species Streptomyces coelicolor is performed, of which 249 profiles are newly generated for S. coelicolor cultivated on 20 different carbon sources and 64 engineered strains with overexpressed sigma factors. ICA of the transcriptome dataset reveals 117 independently modulated groups of genes (iModulons), which account for 81.6% of the variance in the dataset. The genes in each iModulon are involved in specific cellular responses, which are often transcriptionally controlled by specific regulators. Also, iModulons accurately predict 25 secondary metabolite biosynthetic gene clusters encoded in the genome. This systemic analysis leads to reveal the functions of previously uncharacterized genes, putative regulons for 40 transcriptional regulators, including 30 sigma factors, and regulation of secondary metabolism via phosphate‐ and iron‐dependent mechanisms in S. coelicolor. ICA of large transcriptomic datasets thus enlightens a new and fundamental understanding of transcriptional regulation of secondary metabolite synthesis along with interconnected metabolic processes in Streptomyces.

show abstract

Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans

Cited by 15 publications

References 12 publications

Biosynthesis of aminocyclitol-aminoglycoside antibiotics and related compounds

Biosynthesis of aminocyclitol-aminoglycoside antibiotics and related compounds

Identification of the duplicated genes for S ‐adenosyl‐ l ‐methionine synthetase ( metK1‐sp and metK2‐sp ) in Streptomyces peucetius var. caesius ATCC 27952

Machine‐Learning Analysis of Streptomyces coelicolor Transcriptomes Reveals a Transcription Regulatory Network Encompassing Biosynthetic Gene Clusters

Contact Info

Product

Resources

About