Expression of 19 microRNAs in glioblastoma and comparison with other brain neoplasia of grades I–III

Abstract

Several biomarkers have been proposed as useful parameters to better specify the prognosis or to delineate new target therapy strategies for glioblastoma patients. MicroRNAs could represent putative target molecules, considering their role in tumorigenesis, cancer progression and their specific tissue expression. Although several studies have tried to identify microRNA signature for glioblastoma, a microRNA profile is still far from being well-defined. In this work the expression of 19 microRNAs (miR-7, miR-9, miR-9∗, miR-10a, miR-10b, miR-17, miR-20a, miR-21, miR-26a, miR-27a, miR-31, miR-34a, miR-101, miR-137, miR-182, miR-221, miR-222, miR-330, miR-519d) was evaluated in sixty formalin-fixed and paraffin-embedded glioblastoma samples using a locked nucleic acid real-time PCR. Moreover, a comparison of miRNA expressions was performed between primary brain neoplasias of different grades (grades IV-I). The analysis of 14 validated miRNA expression in the 60 glioblastomas, using three different non-neoplastic references as controls, revealed a putative miRNA signature: mir-10b and miR-21 were up-regulated, while miR-7, miR-31, miR-101, miR-137, miR-222 and miR-330 were down-regulated in glioblastomas. Comparing miRNA expression between glioblastoma group and gliomas of grades I-III, 3 miRNAs (miR-10b, mir-34a and miR-101) showed different regulation statuses between high-grade and low-grade tumors. miR-10b was up-regulated in high grade and significantly down-regulated in low-grade gliomas, suggesting that could be a candidate for a GBM target therapy. This study provides further data for the identification of a miRNA profile for glioblastoma and suggests that different-grade neoplasia could be characterized by different expression of specific miRNAs.

Comprehensive analysis of microRNA expression profile in malignant glioma tissues

“…According to the meta‐analysis results, miR‐21, miR‐10b and miR‐25 are the most frequently up‐regulated, whereas miR‐139‐5p, miR‐218 and miR‐124‐3p are commonly down‐regulated in GBM patients. Both miR‐21 and miR‐10b belong to the best characterized miRNAs influencing malignant glioma development (Karsy et al., 2012; Mizoguchi et al., 2012; Purow, 2011) and are acclaimed to be up‐regulated in high‐grade gliomas (Visani et al., 2014). On the contrary, miR‐25 is rarely recognized as an important player in glioma biology, despite its frequent up‐regulation showed in profiling studies.…”

MiR‐31 and miR‐128 regulates poliovirus receptor‐related 4 mediated measles virus infectivity in tumors

“…The increase in PVRL4 protein was observed with no change in PVRL4 mRNA levels suggesting that PVRL4 is post‐transcriptionally regulated. We and others (Karsy et al., 2012; Mizoguchi et al., 2012; Visani et al., 2014) have shown that miR‐31 and miR‐128 expression levels are down‐regulated in glioblastoma. We further observed negative correlations between the expression levels of miR‐31/‐128 and PVRL4 protein levels in glioblastoma patient samples.…”

Serum miR‐182 is a predictive biomarker for dichotomization of risk of hepatocellular carcinoma in rats

“…Longitudinal analysis of circulating miRNAs revealed rno‐miR‐182 as a potential predictive biomarker, which is 96% identical in sequence to its human homolog. miR‐182 is another oncomiR that is commonly upregulated in multiple cancers and predicts poor survival in patients . Chen et al observed that high levels of serum miR‐182 in patients with HCC were significantly associated with decreased survival after surgery.…”