Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma

Iliev, Robert; Fedorko, Michal; Macháčková, Táňa; Mlčochová, Hana; Svoboda, Marek; Pacík, Dalibor; Doležel, Jan; Staník, Michal; Slabý, Ondřej

doi:10.21873/anticanres.11239

Cited by 78 publications

(70 citation statements)

References 15 publications

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“…In 2015, Yang and colleagues (9) firstly described the presence of piR-57125 (DQ596014) in serum and plasma and proved that similarly to miRNAs, this piRNA remains extremely stable regardless of repetitive freeze-thawing or long-term incubation at room temperature. Since that time, several other articles described the presence of piRNAs in circulation and their deregulation in blood samples of cancer patients (8,20,35).…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, the authors further revealed that this piRNA plays a tumorpromoting role by upregulating phosphorylation and transcriptional activity of HSF1, the common transcription factor of heat shock proteins (19). Interestingly, piR-823 was also observed to be deregulated not only in tissue, but also in blood serum of patients with renal cell carcinoma (8) and gastric cancer (20). Currently, next-generation sequencing (NGS) is widely used to identify known as well as novel piRNAs with deregulated expression in cancer.…”

Section: Introductionmentioning

confidence: 93%

“…Recently, several studies have indicated the abundance of novel class of small noncoding RNAs called PIWI-interacting RNAs (piRNA) in various types of body fluids (8,9). These 26-32 nucleotide-long molecules have been shown to participate in the epigenetic regulation of cancer and other diseases and are key elements of cellular homeostasis (10).…”

Section: Introductionmentioning

confidence: 99%

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Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer

Vychytilova-Faltejskova

Štítkovcová

Radová

et al. 2018

Cancer Epidemiology, Biomarkers &Amp; Prevention

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The early detection of colon cancer is one of the main prerequisites for successful treatment and mortality reduction. Circulating PIWI-interacting RNAs (piRNA) were recently identified as novel promising biomarkers. The purpose of the study was to assess the profiles of piRNAs in blood serum of colon cancer patients with the aim to identify those with high diagnostic potential. Blood serum samples from 403 colon cancer patients and 276 healthy donors were included in this 3-phase biomarker study. Large-scale piRNA expression profiling was performed using Illumina small RNA sequencing. The diagnostic potential of selected piRNAs was further validated on independent training and validation sets of samples using RT-qPCR. In total, 31 piRNAs were found to be significantly deregulated in serum of cancer patients compared with healthy donors. Based on the levels of piR-5937 and piR-28876, it was possible to differentiate between cancer patients and healthy donors with high sensitivity and specificity. Moreover, both piRNAs exhibited satisfactory diagnostic performance also in patients with stage I disease and enabled detection of colon cancer with higher sensitivity than currently used biomarkers CEA and CA19-9. Finally, the expression of analyzed piRNAs in blood restored significantly 1 month after the surgical resection. Based on our findings, piRNAs are abundant in human blood serum. Furthermore, their levels in colon cancer have been observed to be significantly deregulated. However, their involvement in carcinogenesis must be further established. piRNAs could serve as promising noninvasive biomarkers for early detection of colon cancer. .

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer

Vychytilova-Faltejskova

Štítkovcová

Radová

et al. 2018

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Further, xenograft nude mice model confirmed its tumor suppressive role in a dose-dependent manner, indicating that piR-823 play a crucial role in gastric cancer. Recently, Iliev et al demonstrated that piR-823 is down-regulated in tumor tissue, blood serum and urine of renal cell carcinoma (RCC) [86]. Law et al [87] identified a novel piR-Hep1 which is up-regulated in hepatocellular carcinoma (HCC) compared to adjacent non-malignant liver tissues through deep sequencing.…”

Section: Introductionmentioning

confidence: 99%

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

Han

Xia

et al. 2017

Cell Physiol Biochem

124

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P-Element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are a type of noncoding RNAs (ncRNAs) and interact with PIWI proteins. piRNAs were primarily described in the germline, but emerging evidence revealed that piRNAs are expressed in a tissue-specific manner among multiple human somatic tissue types as well and play important roles in transposon silencing, epigenetic regulation, gene and protein regulation, genome rearrangement, spermatogenesis and germ stem-cell maintenance. PIWI proteins were first discovered in Drosophila and they play roles in spermatogenesis, germline stem-cell maintenance, self-renewal, retrotransposons silencing and the male germline mobility control. A growing number of studies have demonstrated that several piRNA and PIWI proteins are aberrantly expressed in various kinds of cancers and may probably serve as a novel biomarker and therapeutic target for cancer treatment. Nevertheless, their specific mechanisms and functions need further investigation. In this review, we discuss about the biogenesis, functions and the emerging role of piRNAs and PIWI proteins in cancer, providing novel insights into the possible applications of piRNAs and PIWI proteins in cancer diagnosis and clinical treatment.

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“…Snížené hladiny této piRNA byly zaznamenány u nádorů žaludku [59] či u pa cientů s renálním karcinomem [60], přičemž umělé navýšení hladiny piR-823 in vitro v buněčné linii odvozené od nádoru žaludku vedlo k významné inhibici růstu nádorových buněk. Nádorově supresorový efekt této piRNA byl následně potvrzen in vivo s využitím myšího modelu [61]. K opačným výsledkům dospěla studie zabývající se expresí piR-823 u mnohočetného myelomu.…”

Section: Piwi Proteiny a Kancerogenezeunclassified

Involvement of PIWI-interacting RNAs in Cancerogenesis via the Regulation of Gene Expression

Rybecká¹,

Štítkovcová²,

Vychytilová-Faltejsková³

et al. 2016

Klin Onkol

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Východiska: Za poslední desetiletí přibyly stovky studií, jež souhlasně prokázaly, že krátké nekódující RNA jsou slibnými dia gnostickými, prognostickými a prediktivními bio markery nádorových onemocnění. Významnou podskupinu těchto molekul představují RNA interagující s PIWI proteiny, označované jako piRNA. Tyto krátké RNA se podílejí na regulaci genové exprese na transkripční nebo post-transkripční úrovni, jejich hlavní úlohou je však epigenetické umlčování transpozibilních elementů LINE a SINE, čímž významně přispívají k udržení genomové stability. Dále se účastní důležitých buněčných procesů, jakými jsou gametogeneze, segregace chromozomů či sebeobnova kmenových buněk. Přestože byly piRNA poprvé detekovány v zárodečných buňkách, bylo zjištěno, že se vyskytují ve vysokých hladinách také v jiných lidských tkáních, přičemž jejich exprese vykazuje tkáňovou specificitu. První studie rovněž prokázaly změněný expresní profil piRNA u pa cientů s nádorovými onemocněními. Funkce těchto molekul v procesu kancerogeneze však zatím zůstávají neobjasněné. Do popředí zájmu se v poslední době též dostávají volné cirkulující piRNA v tělních tekutinách, které nabízejí široké využití pro včasný záchyt nádorových onemocnění, predikci léčebné odpovědi či stanovení prognózy pa cientů. Cíl: Tento přehledový článek jako první v českém jazyce shrnuje dosavadní poznatky o bio genezi piRNA, o jejich strategii při umlčování transpozibilních elementů a dalších genů. Poskytuje rovněž ucelený přehled o piRNA s deregulovanou expresí u lidských nádorových onemocnění a klade důraz na jejich potenciální dia gnostické a terapeutické využití. Klíčová slova PIWI-interagující RNA-piRNA-bio geneze-nádorové onemocnění-umlčování transpozonů-bio markery-terapeutické cíle Výsledky tohoto výzkumu byly získány v rámci projektu CEITEC 2020 (LQ1601) za finančního přispění Ministerstva školství, mládeže a tělovýchovy České republiky v rámci účelové podpory z prostředků Národního programu udržitelnosti II. The results of this research have been acquired within CEITEC 2020 (LQ1601) project with financial contribution made by the Ministry of Education, Youths and Sports of the Czech Republic within special support paid from the National Programme for Sustainability II funds. Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy. The authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study. Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do bi omedicínských časopisů. The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers.

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Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma

Cited by 78 publications

References 15 publications

Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer

Circulating PIWI-Interacting RNAs piR-5937 and piR-28876 Are Promising Diagnostic Biomarkers of Colon Cancer

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

Involvement of PIWI-interacting RNAs in Cancerogenesis via the Regulation of Gene Expression

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