Expression Levels of Mir-21, Mir-146B and Mir-326 As Potential Biomarkers in Behcet'S Disease

Jadideslam, Golamreza; Ansarin, Khalil; Sakhinia, Ebrahim; Babaloo, Zohreh; Abhari, Alireza; Alipour, Shahriar; Farhadi, Jafar; Shirvani, Sam Seydi; Ghojazadeh, Morteza; Khabbazi, Alireza

doi:10.2217/bmm-2019-0098

Cited by 22 publications

(14 citation statements)

References 36 publications

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“… 26 For example, miR-326 expression could be used as a potential biomarker in patients with Behcet's disease. 27 Serum miR-21 and miR-221 were used as biomarkers for cerebrovascular diseases. 28 Besides, miR-30-5p might be a clinical biomarker for AS.…”

Section: Discussionmentioning

confidence: 99%

Serum MicroRNA-137 Serves as a Novel Biomarker for Cerebral Atherosclerosis Diagnosis and Cerebrovascular Event Prediction

Xuan

Shang

2021

Journal of Cardiovascular Pharmacology

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: MicroRNAs have been reported as biomarkers for various diseases, including cerebral atherosclerosis (AS). In this study, whether serum microRNA-137 (miR-137) could be used as a biomarker for diagnosing cerebral AS and predicting cerebrovascular event was investigated. Quantitative real-time PCR was used to measure the expression of miR-137 in serum. Logistic analysis was used to evaluate the risk factors for the occurrence of cerebral AS, and receiver operating characteristic curves were used to estimate the diagnostic value of miR-137 and other risk factors for AS occurrence. Furthermore, the prognostic value of miR-137 for patients with AS was estimated using Kaplan–Meier survival analysis and Cox regression analysis. The results indicated that serum miR-137 levels were decreased in patients with cerebral AS. The expression of miR-137 was negatively correlated with total cholesterol and low-density lipoprotein cholesterol levels in patients with cerebral AS. The levels of miR-137, total cholesterol, low-density lipoprotein cholesterol, and hypersensitivity C response protein may serve as risk factors for the occurrence of cerebral AS, and miR-137 had diagnostic value for AS screening. Cerebral AS patients with positive cerebrovascular events have low miR-137 expression. Patients with high miR-137 expression had a lower incidence of cerebrovascular adverse events (log-rank P = 0.013), and miR-137 was an independent prognostic marker for the prediction of cerebrovascular event occurrence in patients with cerebral AS. In conclusions, our findings indicate that serum miR-137 levels are decreased in patients with cerebral AS and may be a new biomarker for diagnosing cerebral AS and predicting cerebrovascular events.

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Section: Discussionmentioning

confidence: 99%

Serum MicroRNA-137 Serves as a Novel Biomarker for Cerebral Atherosclerosis Diagnosis and Cerebrovascular Event Prediction

Xuan

Shang

2021

Journal of Cardiovascular Pharmacology

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“…We subsequently verified the expression levels of a set of selected candidate miRNAs that are related to allergic and autoimmune diseases, such as upregulated miRNAs (miR-326, miR-142-5p) and downregulated miRNAs (miR-644a, miR-181a2-3p) [ 31 , 32 , 33 ], by qRT-PCR in LS tissues from the same patients examined by RNASeq ( Figure 2 A) and from an additional cohort of LS patients by qRT-PCR. Our data showed that the upregulated miRNAs, miR-326, miR-142-5p and downregulated miRNAs, miR-644a, miR-181a-2-3p were concordant with our RNASeq data ( Figure 2 B).…”

Section: Resultsmentioning

confidence: 99%

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

Tan

Ren

Yang

et al. 2021

Cells

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Lichen sclerosus (LS) is a chronic inflammatory skin disorder with unknown pathogenesis. The aberrant expression of microRNAs (miRNAs) is considered to exert a crucial role in LS. We used the next-generation sequencing technology (RNASeq) for miRNA profiling and Ingenuity Pathway Analysis (IPA) for molecular network analysis. We performed qRT-PCR, miRNA transfection and Matrigel assays for functional studies. We identified a total of 170 differentially expressed miRNAs between female LS and matched adjacent normal tissue using RNASeq, with 119 upregulated and 51 downregulated. Bioinformatics analysis revealed molecular networks that may shed light on the pathogenesis of LS. We verified the expression of a set of miRNAs that are related to autoimmunity, such as upregulated miR-326, miR-142-5p, miR-155 and downregulated miR-664a-3p and miR-181a-3p in LS tissue compared to the matched adjacent normal tissue. The differentially expressed miRNAs were also verified in blood samples from LS patients compared to healthy female volunteers. Functional studies demonstrated that a forced expression of miR-142-5p in human dermal fibroblast PCS-201-010 cells resulted in decreased cell proliferation and migration. These findings suggest that differentially expressed miRNAs may play an important role in LS pathogenesis; therefore, they could serve as biomarkers for LS management.

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“…More recently, Jadideslam and colleagues compared the expression of miR-21, miR-146b, and miR-326 in PBMCs from Iranian Azari BD patients with that in healthy controls, and revealed the downregulation of miR-21 and upregulation of miR-326. Furthermore, they suggested the great potential of miR-326 as a biomarker for predicting the uveitis and severe eye involvement in BD (Jadideslam et al, 2019).…”

Section: Micrornas and Behcet's Diseasementioning

confidence: 99%

MicroRNAs and Autoimmune-Mediated Eye Diseases

Wei

Zhao

et al. 2020

Front. Cell Dev. Biol.

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MicroRNAs (miRNAs) are evolutionarily conserved short non-coding RNAs that act at post-transcriptional regulation of gene expression by destroying target messenger RNA or inhibiting its translation. Recently, miRNAs have been identified as important regulators in autoimmunity. Aberrant expression and function of miRNAs can lead to dysfunction of immune system and mediate autoimmune disorders. Here, we summarize the roles of miRNAs that have been implicated in three representative ocular autoimmune disorders, including autoimmune uveitis, Grave's ophthalmopathy, and Sjögren's syndrome dry eye, and discuss the potential of miRNAs as biomarkers and therapeutic targets for the diagnosis and treatment of these diseases.

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Expression Levels of Mir-21, Mir-146B and Mir-326 As Potential Biomarkers in Behcet'S Disease

Cited by 22 publications

References 36 publications

Serum MicroRNA-137 Serves as a Novel Biomarker for Cerebral Atherosclerosis Diagnosis and Cerebrovascular Event Prediction

Serum MicroRNA-137 Serves as a Novel Biomarker for Cerebral Atherosclerosis Diagnosis and Cerebrovascular Event Prediction

Differentially Regulated miRNAs and Their Related Molecular Pathways in Lichen Sclerosus

MicroRNAs and Autoimmune-Mediated Eye Diseases

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