Expression levels and association of gelatinases MMP-2 and MMP-9 and collagenases MMP-1 and MMP-13 with VEGF in synovial fluid of patients with arthritis

Abstract

This study was performed to provide evidence, albeit indirectly, as to which matrix metalloproteinases (MMPs), among the gelatinases MMP-2 and MMP-9 and the collagenases MMP-1 and MMP-13, play a more proactive role in the angiogenic process in arthritic joint. Joint fluid was collected from 33 patients with rhuematoid arthritis (RA) and osteoarthritis (OA), and protein (MMPs and vascular endothelial growth factor (VEGF)) levels were measured by ELISA, and the association of MMPs with VEGF was evaluated in joint fluid of patients with RA or OA. The levels of collagenases (total MMP-1 and total MMP-13) and gelatinases (total MMP-2 and total MMP-9) in RA joint fluid were significantly higher than those in OA fluid. Total MMP-9 levels were significantly associated with VEGF levels in RA fluids, but not in OA fluid, while total MMP-13 levels were strongly associated with VEGF levels in both RA and OA fluid. However, total MMP-2 and total MMP-1 levels were not associated with VEGF levels in either RA or OA joint fluid. Our results indirectly suggest that in RA and OA, MMP-9 and MMP-13 may play a more important role in angiogenesis than MMP-2 and MMP-1.

Correlation between interleukin-6 expression in articular cartilage bone and osteoarthritis

“…A possible mechanism is the elevated MMP-9 secretion induced by higher IL-6 expression from monocytes, which is activated by the synovial inflammatory response, thereby disturbing the balance between MMPs and TIMPs and leading to the damage of chondrocytes (Guo et al, 2011). In this process, IL-6 stimulates the production of prostaglandin and collagenase in normal cartilage and synovial cells in order to stimulate the IL-1 induced-MMP response in synovial fibroblasts in rheumatoid arthritis (Litinsky et al, 2006;Wang et al, 2010;Shen et al, 2011) or to elevate the expression of VEGF (vascular endothelial growth factor) type II receptor, thus facilitating MMP-9 secretion (Sakao et al, 2008;Kim et al, 2011).…”

Upcoming value of gene expression analysis in rheumatology

“…Повышенную продукцию различных ММР наблюдали в синовиальной жидкости и синовиальных фибробластах тканей суставов больных РА при воспалении [130]. Макрофагальный ингибиторный фактор миграции, который продуцируется преимущественно макрофагами в ответ на различные провоспалительные стимулы, способен повышать экспрессию ММР-1 и -3 в культуре синовиальных фибробластов больных РА [131], тогда как экспрессия ММР-9 и -13 в суставной жидкости больных РА ассоциируется с концентрацией сосудистого эндотелиального фактора роста (VEGF), поэтому эти ММР могут участвовать в процессах ангиогенеза [132]. Протеиназы также участвуют в разрушении суставов при ОА [133], спондилоартропатиях [134], системном склерозе [135] и СКВ [136].…”

“…[источник] TNFa [14] S100 Са-связывающий белок А8 [89] MMP-9 [95] IL-1b [90] ASICs [75] MMP-12 [128] IL-7R [142] TPRV1 [76,77] RANKL [116,117] IL-2 индуцируемая киназа Т-лимфоцитов [157] NGF [80] OPG [114] Регуляторы активации Т-лимфоцитов [157] Рецептор лимфотоксина [81] Катепсины В, К, L [125] Хемокины и их рецепторы [157] Эндоканнабиноиды [82] MMP-1 [129] р53-связывающий белок [150] Антагонисты PPARa [82] MMP-3 [130] Рецептор Т-лимфоцитов [157] Опиоидные рецепторы [84] MMP-13 [132] Регуляторы активации Т-лимфоцитов [157] Антагонисты опиоидных рецепторов [84] Cелектин [150] Рецептор фракталкина CX3CR1 [86] RUNX3 [113] IL6 [31] ULK1 [96,140] …”

Covalent linking peptide to hydrothermally reduced graphene oxide for ultrasensitive detection of matrix metalloproteinase 9

“…It was reported that MMP9 concentration in the synovial fluid of patients with osteoarthritis was 3.47-9.77 pM (Kim et al 2010) and MMP9 concentration in the cerebrospinal fluid of patients after epileptic seizures was 50-102 pM (Li et al 2013). MMP9 below 150 pM cannot be analyzed by GO-based FRET sensor developed previously (Nguyen et al 2017).…”