Expression level of Ubc9 protein in rat tissues.

Abstract

Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDa band of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein appears to have some degree of tissue specificity.

Protein SUMOylation is Massively Increased in Hibernation Torpor and is Critical for the Cytoprotection Provided by Ischemic Preconditioning and Hypothermia in SHSY5Y Cells

“…Since little (or no) analysis of SUMO tissue distribution has been performed, it is unclear whether the distributions noted here are typical of most vertebrate tissues or are restricted to hibernating animals. However, comparison of rat Ubc9 expression levels in various tissues (Golebiowski et al, 2003) showed interesting parallels with SUMO-1 tissue distributions observed in our ground squirrels that is, the highest expression in spleen, relatively high expression in kidney and liver, and low expression in heart and skeletal muscle.…”

Functional Analysis and Post-Transcriptional Regulation of Oocyte-Specific Transcription Factors During Early Embryogenesis in Cattle

“…As shown in Fig. 1A, UBC9 transcripts were expressed ubiquitously expressed in bovine tissues including fetal and adult ovaries, consistent with the expression pattern observed in mouse (Azuma et al, 2001), rat (Golebiowski et al, 2003) and human (Yasugi and Howley, 1996),…”

ERβ targets ZAK and attenuates cellular hypertrophy via SUMO‐1 modification in H9c2 cells

“…The findings suggest that ERβ blocked ZAK nuclear translocation to prevent ZAK‐induced hypertrophy by modulating the SUMOylation of ZAK. In addition, the fact that the components of the SUMO conjugation pathway are abundant in the heart indicates the possibility that the SUMO pathway may be implicated in cardiovascular development via modifying the transcription factors indispensable for normal cardiovascular function and development . The results show that Dox treatment to overexpress ZAK induced SUMO‐1 protein expression; however, overexpressed ERβ could totally repress the SUMO‐1 protein level induced by ZAK.…”