Expression kinetics of ISG15 and viral major capsid protein (VP2) in Atlantic cod (Gadus morhua L.) fry following infection with infectious pancreatic necrosis virus (IPNV)

Das, Basanta Kumar; Collet, Bertrand; Snow, Michael; Ellis, A.E.

doi:10.1016/j.fsi.2007.03.012

Cited by 23 publications

(11 citation statements)

References 22 publications

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“…These experiments verified that Atlantic salmon ISG15 is a functional homologue of mammalian ISG15, suggesting that it has potential antiviral activity and that it fulfils a role in innate immunity. IPNV has also been shown to be a potent inducer of ISG15 in Atlantic cod (Gadus morhua L.) [49]. Indeed, our results show that the pcDNA-VP2 vaccine can stimulate the expression of ISG15 in brown trout 7 and 15 days after injection, with a similar yet stronger pattern than that obtained after IPNV infection.…”

Section: Discussionsupporting

confidence: 69%

In vitro and in vivo immune responses induced by a DNA vaccine encoding the VP2 gene of the infectious pancreatic necrosis virus

Heras

Prieto

Saint-Jean

2009

Fish & Shellfish Immunology

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The work presented here describes the construction of a plasmid encoding the VP2 gene of the infectious pancreatic necrosis virus (IPNV), its expression in BF-2 cells and an evaluation of its activity in brown trout (Salmo trutta L) soon after injection. Preliminary experiments to evaluate the potential of the plasmid to induce neutralizing antibodies were also performed. We established a BF-2 cell line that expresses VP2 constitutively and we examined the infection of these VP2-transfected BF-2 cells with homologous and heterologous viruses. The expression kinetics of IFN, and of the IFN-induced genes Mx and ISG15, was also evaluated in brown trout over a 15 day interval, and quantified by real-time or semi-quantitative PCR. Type I IFN and Mx are markers of the non-specific innate immune response to viruses and they are involved in antiviral defences. Our results demonstrate that expression of the IPNV VP2 protein in BF-2 cells induces an antiviral state against IPNV and against the infectious haematopoietic necrosis virus (IHNV). In BF-2 infected cells, VP2 inhibited both the IPNV and IHNV-induced cytopathic effect to some extent, as well as the virus yield. In vivo, VP2 was expressed in haematopoietic tissues such as the head kidney of 7 month-old trout. In addition, it induced early immune responses and specific immunity 30 days after injection. IFN mRNA expression increased sharply on the 1st and 15th day post-injection and expression of other IFN-induced genes as Mx and ISG15 was also detected soon after vaccination of brown trout. Moreover, specific antibodies were detected 30 days after vaccination. These results suggest that the VP2 gene is a good candidate for the design of IPNV-DNA vaccines and to investigate the use of cytokines as co-stimulatory molecules.

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Section: Discussionsupporting

confidence: 69%

In vitro and in vivo immune responses induced by a DNA vaccine encoding the VP2 gene of the infectious pancreatic necrosis virus

Heras

Prieto

Saint-Jean

2009

Fish & Shellfish Immunology

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show abstract

“…ISG15 protein is highly expressed after stimulating mammalian cells with IFNb [32]. IPNV could induce ISG15 gene expression and remained high even up to 21 days post-infection in Atlantic cod [33]. In our study, immune-related genes, including ifna, ifnr, mx, isg15 and several interferon regulatory factors, were upregulated following IPNV infection (Fig.…”

Section: Discussionsupporting

confidence: 54%

Activation of cytokine expression occurs through the TNFα/NF-κB-mediated pathway in birnavirus-infected cells

Wang

Liu

Gong

et al. 2011

Fish & Shellfish Immunology

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“…Studies with mammalian models have indicated that ISG15 possesses broadspectrum antiviral activity and can inhibit the production of a wide range of DNA and RNA viruses [6]. In fish, it is known that virus infection upregulates the expression of ISG15 in Atlantic cod [10,29], Japanese flounder [30], Atlantic salmon [8,31], and crucian carp [11]. Similarly, poly(I:C) treatment was reported to enhance ISG15 expression in black rockfish [13], Atlantic cod [9], and channel catfish [12].…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection

Liu

Sun

Zhang

et al. 2010

Fish & Shellfish Immunology

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Expression kinetics of ISG15 and viral major capsid protein (VP2) in Atlantic cod (Gadus morhua L.) fry following infection with infectious pancreatic necrosis virus (IPNV)

Cited by 23 publications

References 22 publications

In vitro and in vivo immune responses induced by a DNA vaccine encoding the VP2 gene of the infectious pancreatic necrosis virus

In vitro and in vivo immune responses induced by a DNA vaccine encoding the VP2 gene of the infectious pancreatic necrosis virus

Activation of cytokine expression occurs through the TNFα/NF-κB-mediated pathway in birnavirus-infected cells

Identification and analysis of a Sciaenops ocellatus ISG15 homologue that is involved in host immune defense against bacterial infection

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