Expression kinetics and plasmid stability of recombinant E. coli encoding urease-derived peptide with bioinsecticide activity

Optimization and kinetic characterization of recombinant 1,3-β-glucanase production in Escherichia coli K-12 strain BL21/pETSD10 - a bioreactor scale study

“…At very low aeration values (0Á18 vvm), the specific activity was very low. Similar results were obtained in the experiments with recombinant strains of Staphylococcus warneri EX17 producing lipase (Volpato et al 2009 (Tomazetto et al 2007;C ßalik et al 2010;Bellido et al 2013). In these studies, the authors assumed that under deficient oxygen concentrations, the production of the protein and metabolite is deteriorated significantly.…”

Insecticidal effect of Canavalia ensiformis major urease on nymphs of the milkweed bug Oncopeltus fasciatus and characterization of digestive peptidases

“…Biophysics, Universidade Federal de São Paulo, São Paulo, Brazil). The recombinant peptide Jaburetox-2Ec and anti-Jaburetox2Ec polyclonal antibodies raised in rabbits were prepared as described previously Tomazzeto et al, 2007). Chemicals and reagents used for high performance liquid chromatographics (HPLC) and nano-liquid tandem mass spectrometry (LC-MS/MS) analyses were HPLC or sequencing grade.…”

“…Jaburetox-2Ec, a recombinant peptide equivalent to pepcanatox (Mulinari et al, 2004, is a potent insecticide but does not affect mice or neonate rats when administered by oral or intraperitoneal routes . This recombinant peptide also kills insects that are resistant to intact ureases, such as Spodoptera frugiperda or Triatoma infestans adults (Tomazzeto et al, 2007).…”

Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: Role in the release of entomotoxic peptides from Canavalia ensiformis urease

“…The recombinant urease-derived entomotoxic peptide Jaburetox2Ec, produced and purified as described in Mulinari et al (2007), was used to immunize rabbits and prepare anti-Jaburetox-2Ec polyclonal antibodies (Tomazzeto et al, 2007).…”

“…A recombinant peptide, Jaburetox-2Ec equivalent to pepcanatox, obtained by heterologous expression in Escherichia coli (Mulinari et al, 2004, is highly insecticidal and did not affect mice or neonate rats when administered by oral or intraperitoneal routes . Interestingly, Jaburetox-2Ec kills insects that are resistant to intact ureases, such as Spodoptera frugiperda or adults of Triatoma infestans (Tomazzeto et al, 2007).…”