Expression in murine and human neuroblastoma cell lines of VGF, a tissue specific protein

Rossi, A.; Granata, Filippo; Augusti-Tocco, Gabriella; Canu, Nadia; Levi, Andrea; Possenti, Roberta

doi:10.1016/0736-5748(92)90053-3

Cited by 17 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endocrine cell types that express VGF include posterior pituitary, as well as neuroendocrine and endocrine cells of the anterior pituitary, adrenal medulla, gastrointestinal tract and b-cells pancreatic islets [25,75]. Consistent with the reported VGF distribution, expression of VGF in cultured cell lines is limited to those derived from neuronal and neuroendocrine tissues [72].…”

Section: The Melanocortin Systemmentioning

confidence: 50%

The role of the vgf gene and VGF-derived peptides in nutrition and metabolism

et al. 2007

Self Cite

View full text Add to dashboard Cite

Energy homeostasis is a complex physiological function coordinated at multiple levels. The issue of genetic regulation of nutrition and metabolism is attracting increasing interest and new energy homeostasis-regulatory genes are continuously identified. Among these genes, vgf is gaining increasing interest following two observations: (1) VGF-/- mice have a lean and hypermetabolic phenotype; (2) the first VGF-derived peptide involved in energy homeostasis, named TLQP-21, has been identified. The aim of this review will be to discuss the role of the vgf gene and VGF derived peptides in metabolic and nutritional functions. In particular we will: (1) provide a brief overview on the central systems regulating energy homeostasis and nutrition particularly focusing on the melanocortin system; (2) introduce the structure and molecular characteristic of vgf; (3) describe the phenotype of VGF deficient mice; (4) present recent data on the metabolic role of VGF-derived peptides, particularly focusing on one peptide named TLQP-21.

show abstract

Section: The Melanocortin Systemmentioning

confidence: 50%

The role of the vgf gene and VGF-derived peptides in nutrition and metabolism

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…ID2 downregulation by the two microRNAs might mediate, at least partly, this reduction, for instance by restoring RB control on proliferation. We then analysed the expression of two proteins modulated during NB cell differentiation: N-Myc, which is downregulated [44], and Vgf – a neuropeptide precursor induced by neurotrophins [45] –, which is upregulated by RA in SK-N-BE cells [46], [47]. Induction of vgf expression involves binding of a bHLH protein complex to the promoter [45].…”

Section: Resultsmentioning

confidence: 99%

“…ID2, N-Myc and GAPDH antibodies were from Santa Cruz Biotechnology (sc-489, sc-56729 and sc-32233, respectively), Actin and Neurofilament 200 (NF200) antibodies were from Sigma (A2066 and N0142), and Vgf antibody was as described [45], [47]. Horseradish peroxidase secondary antibodies were from Chemicon and Protein A peroxidase from Sigma.…”

Section: Methodsmentioning

confidence: 99%

A New Module in Neural Differentiation Control: Two MicroRNAs Upregulated by Retinoic Acid, miR-9 and -103, Target the Differentiation Inhibitor ID2

et al. 2012

View full text Add to dashboard Cite

The transcription factor ID2 is an important repressor of neural differentiation strongly implicated in nervous system cancers. MicroRNAs (miRNAs) are increasingly involved in differentiation control and cancer development. Here we show that two miRNAs upregulated on differentiation of neuroblastoma cells – miR-9 and miR-103 – restrain ID2 expression by directly targeting the coding sequence and 3′ untranslated region of the ID2 encoding messenger RNA, respectively. Notably, the two miRNAs show an inverse correlation with ID2 during neuroblastoma cell differentiation induced by retinoic acid. Overexpression of miR-9 and miR-103 in neuroblastoma cells reduces proliferation and promotes differentiation, as it was shown to occur upon ID2 inhibition. Conversely, an ID2 mutant that cannot be targeted by either miRNA prevents retinoic acid-induced differentiation more efficient than wild-type ID2. These findings reveal a new regulatory module involving two microRNAs upregulated during neural differentiation that directly target expression of the key differentiation inhibitor ID2, suggesting that its alteration may be involved in neural cancer development.

show abstract

“…Neuroblastoma cells were identified as VGF positive and the VGF expression is upregulated during differentiation [43]. VGF peptides are present in endocrine cells early during development and adulthood and VGF increases in hyperplasia and tumors [41].…”

Section: Discussionmentioning

confidence: 99%

Mass spectrometry identification of granins and other proteins secreted by neuroblastoma cells

et al. 2013

View full text Add to dashboard Cite

We used mass spectrometry-based protein identification to determine the presence of granins and other proteins in the mouse neuroblastoma secretome. We detected polypeptides derived from four members of the granin family: chromogranin A, chromogranin B, secretogranin III, and VGF. Many of them are derived from previously described biologically active regions; however, for VGF and CgB, we detected peptides not related to known bioactivities. Along with granins, we identified 115 other proteins secreted by mouse neuroblastoma cells, belonging to different functional categories. Fifty-six out of 119 detected proteins possess the signal fragments required for translocation into endoplasmic reticulum. Sequences of remaining 63 proteins were analyzed using SecretomeP algorithm to determine probability of nonclassical secretion. Identified proteins are involved in the regulation of cell cycle, proliferation, apoptosis, angiogenesis, proteolysis, and cell adhesion.

show abstract

Expression in murine and human neuroblastoma cell lines of VGF, a tissue specific protein

Cited by 17 publications

References 16 publications

The role of the vgf gene and VGF-derived peptides in nutrition and metabolism

The role of the vgf gene and VGF-derived peptides in nutrition and metabolism

A New Module in Neural Differentiation Control: Two MicroRNAs Upregulated by Retinoic Acid, miR-9 and -103, Target the Differentiation Inhibitor ID2

Mass spectrometry identification of granins and other proteins secreted by neuroblastoma cells

Contact Info

Product

Resources

About