Expression in Escherichia coli of a New Multidrug Efflux Pump, MexXY, from Pseudomonas aeruginosa

Abstract

Two new genes (mexXY) similar to mexAB,mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed withEscherichia coli cells harboring the plasmid carryingmexXY. This MexXY system confers higher resistance to fluoroquinolones than the MexAB and MexCD systems, and E. coli TolC or P. aeruginosa OprM is necessary for the function of the MexXY system.

Adaptive resistance to cationic compounds in Pseudomonas aeruginosa

“…2). These proteins form a functional tripartite efflux machinery that is capable of extruding a variety of antibiotics, including aminoglycosides, macrolides, ␤-lactams (penicillins except carbenicillin and sulbenicillin, cephalosporins except cefsulodin, ceftazidime and carbapenems), quinolones, lincomycin, chloramphenicol, tetracyclines and tigecycline [26][27][28][29].…”

Inactivation of MuxABC-OpmB transporter system in Pseudomonas aeruginosa leads to increased ampicillin and carbenicillin resistance and decreased virulence

“…There are twelve RND-type efflux pumps in P. aeruginosa PAO1 genome and all of them have been characterized to some details in recently years. They include MexAB-OprM (Li et al, 1995Zhao et al, 1998), MexCD-OprJ (Poole et al, 1996, Linares et al, 2005, MexEF-OprN (Kohler et al, 1997;Linares et al, 2005), MexGHI-OpmD (Aendekerk et al, 2002(Aendekerk et al, , 2005Sekiya et al, 2003), MexJK-OprM (Chuanchuen et al, 2002), MexMN , MexPQ-OpmE (Mima et al, 2005), MexVWOprM , MexXY-OprM (Mine et al, 1999;Masuda et al, 2000), CzcCBA (Rensing et al, 1997;Hassan et al, 1999;Perron et al, 2004;Caille et al, 2007), TriABCOpmH (Mima et al, 2007), and MuxABC-OpmB (Mima et al, 2009). The MuxABC-OpmB was the last reported RND system in P. aeruginosa.…”

Alterations of susceptibility of Pseudomonas aeruginosa by overproduction of multidrug efflux systems, MexAB-OprM, MexCD-OprJ, and MexXY/OprM to carbapenems: Substrate specificities of the efflux systems

“…At least four species of tripartite multidrug efflux systems, MexAB-OprM, 1,2 MexCD-OprJ, 3 MexEF-OprN, 4 and MexXY/OprM, 5,6 composed of periplasmic MFS-family protein, RND-family inner membrane protein, and outer membrane efflux protein (OEP), are encoded on the P. aeruginosa chromosome. These efflux systems extrude actively broad antibiotics from the cell interior and contribute to multidrug resistance of this bacterium.…”