Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus

Abstract

We have investigated the bovine immune response to heterologous proteins expressed using a recombinant rinderpest virus (RPV). A new gene unit was created in a cDNA copy of the genome of the vaccine strain of RPV, and an open reading frame inserted that encodes the polymerase (3D pol ) and parts of the capsid protein VP1 from foot-and-mouth disease virus (FMDV). Infectious recombinant RPV was rescued and shown to express the FMDV-derived protein at good levels in infected cells. The rescued virus was only slightly more attenuated in tissue culture than the original virus. Cattle infected with this recombinant generated a normal immune response to RPV, and were protected from lethal challenge by that virus. Experimental animals showed a specific delayed-type hypersensitivity response to FMDV 3D pol , similar to that seen in FMDV infection ; however, no antibodies were detected recognizing either of the components of the FMDV-derived protein, nor was any proliferative response to these epitopes found in isolated peripheral blood lymphocytes from infected animals. No protection was seen against FMDV infection.

Recovery and Characterization of a Chimeric Rinderpest Virus with the Glycoproteins of Peste-des-Petits-Ruminants Virus: Homologous F and H Proteins Are Required for Virus Viability

“…All three pRPV-based plasmids were used in rescue experiments as described previously (4,5). Rescue of each plasmid was tried at least twice, using six 35-mm-diameter wells in each attempt.…”

“…The antibodies used were C1 and C77 (monoclonal mouse anti-RPV H and anti-PPRV H, respectively) (2) and MB18 (rabbit anti-RPV P) (5).…”

“…Virus rescue in 293 cells was as previously described (5). For rescue in Vero cells, a new technique involving use of the recombinant fowlpox virus FP-T7 to express the T7 polymerase protein was used (17).…”

Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication

“…Expression of foreign proteins by several members of the Paramyxoviridae has been reported (Bukreyev et al, 1996;Mebatsion et al, 1996;He et al, 1997;Johnson et al, 1997;Roberts et al, 1998;Baron et al, 1999;Singh & Billeter, 1999;Bailly et al, 2000;Buchholz et al, 2000;Durbin et al, 2000;Krishnamurthy et al, 2000;Huang et al, 2001;Nakaya et al, 2001). In most of these cases, the foreign gene was inserted at a specific position in the viral genome and no systematic attempts were made to examine the effects of the position of the foreign gene on expression levels and virus replication.…”

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus

“…However, feline infectious peritonitis virus vaccination with killed, recombinant or live-attenuated virus results in the production of anti-S Abs, leading to disease enhancement and death after virus challenge (38,39). Although long-lived mucosal immunity, immune enhancement, and recombination with existing strains are potential problems facing the development of an efficacious SARS vaccine, several animal vaccine approaches have proven successful and most SARS patients develop immune responses and recover (2,4,40,41).…”