Expression, functional, and structural analysis of proteins critical for otoconia development

Xu, Yinfang; Zhang, Hui; Ye, Hua; Zhao, Xinbing; Lovas, Sándor; Lundberg, Yunxia Yesha Wang

doi:10.1002/dvdy.22531

Cited by 11 publications

(30 citation statements)

References 13 publications

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“…Recently, Thalmann et al identified Sc1 from mouse otoconia by mass spectrometry ). However, Xu et al (Xu et al 2010) found that Sc1 was hardly detectable in the wild-type otoconia. Instead, the deposition of Sc1 was drastically increased in otoconia crystals when Oc90 is absent, suggesting a possible role for Sc1 as an alternative process of biomineralization (Xu et al 2010).…”

Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 98%

“…However, Xu et al (Xu et al 2010) found that Sc1 was hardly detectable in the wild-type otoconia. Instead, the deposition of Sc1 was drastically increased in otoconia crystals when Oc90 is absent, suggesting a possible role for Sc1 as an alternative process of biomineralization (Xu et al 2010). Sc1 Although Sc1 and Oc90 have no significant sequence similarity, the two proteins share analogous structural features.…”

Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 98%

“…A subsequent in vitro experiment has also demonstrated that Oc90 can facilitate nucleation, determine the crystal size and morphology in a concentration-dependent manner . Recent evidence suggests that the formation of otoconia at all in Oc90 null mice may be partially attributed to the compensatory deposition of Sc1 (Xu et al 2010). …”

Section: Anchoring Proteinsmentioning

confidence: 99%

“…In addition, the enriched cysteines in the polypeptide backbone of Sc1 may enable the formation of numerous intra-and inter-molecular disulfide bridges, as well as dimerization or even oligomerization of the protein, all of which enable the protein to serve as a rigid and stable framework for inorganic crystal deposition and growth (Chun et al 2006;Xu et al 2010).…”

Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 99%

“…The organic components of otoconia primarily consist of glycoproteins and proteoglycans Ito et al 1994;Pisam et al 2002;Pote and Ross 1991;Verpy et al 1999;Wang et al 1998;Xu et al 2010;Zhao et al, 2007). To date, as many as 8 murine otoconins have been identified (Table 1): the predominant otoconial protein, otoconin-90 (Oc90) and other 'minor' otoconins including otolin-1 (aka otolin) (Zhao et al 2007), fetuin-A (aka countertrypin) Zhao et al 2007), osteopontin (aka Spp1) (Sakagami 2000;Takemura et al 1994;Zhao et al 2008a), Sparc-like protein 1 (Sc1, aka hevin and Ecm2) Xu et al 2010), possibly secreted protein acidic and rich in cysteine (Sparc, aka BM-40 and osteonectin), and dentin matrix protein 1 (DMP1). Those otoconins are expressed in different cells and secreted into the utricular and saccular endolymph.…”

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confidence: 99%

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Proteins Involved in Otoconia Formation and Maintenance

Lundberg¹,

Xu²

2012

Otolaryngology

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Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 98%

Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 98%

Section: Anchoring Proteinsmentioning

confidence: 99%

Section: Sc1 Can Partially Compensate the Function Of Oc90mentioning

confidence: 99%

mentioning

confidence: 99%

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Proteins Involved in Otoconia Formation and Maintenance

Lundberg¹,

Xu²

2012

Otolaryngology

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Spatiotemporal differences in otoconial gene expression

Zhang

Lundberg

2016

Genesis

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Otoconia are minute bio-crystals composed of glycoproteins, proteoglycans and CaCO3, and are indispensable for sensory processing in the utricle and saccule. Otoconia abnormalities and degeneration can cause or facilitate crystal dislocation to the ampulla, leading to vertigo and imbalance in humans. In order to better understand the molecular mechanism controlling otoconia formation and maintenance, we have examined the spatial and temporal expression differences of otoconial genes in the mouse inner ear at developmental, mature and aging stages using whole transcriptome sequencing (RNA-Seq) and quantitative RT-PCR. We show that the expression levels of most otoconial genes are much higher in the utricle and saccule compared to other inner ear tissues before postnatal stages in C57Bl/6J mice, and the expression of a few of these genes is restricted to the embryonic utricle and saccule. After the early postnatal stages, expression of all otoconial genes in the utricle and saccule is drastically reduced, while a few genes gain expression dominance in the aging ampulla, indicating a potential for ectopic debris formation in the latter tissue at old ages. The data suggest that the expression of otoconial genes is tightly regulated spatially and temporally during developmental stages and can become unregulated at aging stages.

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Serum vitamin D and recurrent benign paroxysmal positional vertigo

Rhim¹

2016

Laryngoscope Investig Oto

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ObjectivesThe objective of the present study was to examine the effects of serum 25‐hydroxyvitamin D concentrations on patients diagnosed with benign paroxysmal positional vertigo (BPPV) on BPPV recurrence.Study DesignCase series.MethodsA retrospective review of 232 patients diagnosed with BPPV visiting the clinic between June 2014 and June 2015 was performed. All patients underwent a complete otolaryngological, audiologic, and neurologic evaluation. The appropriate particle‐repositioning maneuver was performed depending on the type of BPPV. The patients were divided into the recurrence group and the nonrecurrence group. Age, gender, follow‐up period, type of BPPV, and vitamin D concentrations in the two groups were compared and analyzed through binary logistic regression analyses.ResultsThe average follow‐up period after treatment was 10.2 months. Forty‐one (17.7%) of 232 patients suffered a recurrence during the follow‐up period. The mean vitamin D concentration of 191 patients who did not suffer any recurrence was 16.63 ng/mL, whereas that of 41 patients who suffered a recurrence was 13.64 ng/mL. This difference in vitamin D concentrations was statistically significant (P < 0.019). The patients' age, gender, follow‐up period, and type of BPPV had no statistically significant impact.ConclusionVitamin D is assumed to affect BPPV as a recurrence factor independent of age, gender, follow‐up period, and type of BPPV.Level of Evidence4.

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Expression, functional, and structural analysis of proteins critical for otoconia development

Cited by 11 publications

References 13 publications

Proteins Involved in Otoconia Formation and Maintenance

Proteins Involved in Otoconia Formation and Maintenance

Spatiotemporal differences in otoconial gene expression

Serum vitamin D and recurrent benign paroxysmal positional vertigo

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