Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation

Du, Yaru; Zhang, He; Wang, Hong; Wang, Shuai; Lei, Qiqin; Li, Chao; Kong, Renqiu; Xu, Xudong

doi:10.1101/2020.04.15.042648

Cited by 2 publications

(1 citation statement)

References 43 publications

(72 reference statements)

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“…proposed to be involved in its interaction with PatU3 and, to a lesser extent, with HetR (Videau et al 106 2018; Zhang et al 2018;Du et al 2020). In N. punctiforme, the deletion of the patN gene, which encodes 107 a putative membrane protein, increases heterocyst frequency and decreases the number of vegetative 108 cells between two heterocysts, thus leading to a multiple singular heterocyst (MSH) phenotype (Risser 109 et al 2012) (Fig.…”

Section: Introduction 54mentioning

confidence: 99%

Interaction network among factors involved in heterocyst-patterning in cyanobacteria

Rachedi

Foglino

et al. 2022

Mol Genet Genomics

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The genetically regulated pattern of heterocyst formation in multicellular cyanobacteria represents the simplest model to address how patterns emerge and are established, the signals that control them, and the regulatory pathways that act downstream. Although numerous factors involved in this process have been identified, the mechanisms of action of many of them remain largely unknown. The aim of this study was to identify specific relationships between 14 factors required for cell differentiation and pattern formation by exploring their putative physical interactions in the cyanobacterium model Nostoc sp. PCC 7120 and by probing their evolutionary conservation and distribution across the cyanobacterial phylum. A bacterial two-hybrid assay indicated that 10 of the 14 factors studied here are engaged in more than one protein-protein interaction. The transcriptional regulator PatB was central in this network as it showed the highest number of binary interactions. A phylum-wide genomic survey of the distribution of these factors in cyanobacteria showed that they are all highly conserved in the genomes of heterocyst-forming strains, with the PatN protein being almost restricted to this clade. Interestingly, eight of the factors that were shown to be capable of protein interactions were identified as key elements in the evolutionary genomics analysis. These data suggest that a network of 12 proteins may play a crucial role in heterocyst development and patterning. Unravelling the physical and functional interactions between these factors during heterocyst development will certainly shed light on the mechanisms underlying pattern establishment in cyanobacteria. Response to Reviewers:Dear editor, in this revised version of our manuscript we introduced all the corrections asked by the editor and Reviewer 1. The modifications in the text are marked in yellow, We thank the reviewers and the communicating editor for their valuable reading of our publication. We hope that this revised manuscript will be suited for publication.

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Section: Introduction 54mentioning

confidence: 99%

Interaction network among factors involved in heterocyst-patterning in cyanobacteria

Rachedi

Foglino

et al. 2022

Mol Genet Genomics

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Final Destination? Pinpointing Hyella disjuncta sp. nov. PCC 6712 (Cyanobacteria) Based on Taxonomic Aspects, Multicellularity, Nitrogen Fixation and Biosynthetic Gene Clusters

Jung

D’Agostino

Brust

et al. 2021

Life

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Unicellular cyanobacteria inhabit a wide range of ecosytems and can be found throughout the phylum offering space for taxonomic confusion. One example is strain PCC 6712 that was described as Chlorogloea sp. (Nostocales) and later assigned to the genus Chroococcidiopsis (Chroococcidiopsidales). We now show that this strain belongs to the order Pleurocapsales and term it Hyella disjuncta based on morphology, genome analyses and 16S-23S ITS rRNA phylogeny. Genomic analysis indicated that H. disjuncta PCC 6712 shared about 44.7% orthologue genes with its closest relative H. patelloides. Furthermore, 12 cryptic biosynthetic gene clusters (BGCs) with potential bioactivity, such as a mycosporine-like amino acid BGC, were detected. Interestingly, the full set of nitrogen fixation genes was found in H. disjuncta PCC 6712 despite its inability to grow on nitrogen-free medium. A comparison of genes responsible for multicellularity was performed, indicating that most of these genes were present and related to those found in other cyanobacterial orders. This is in contrast to the formation of pseudofilaments—a main feature of the genus Hyella—which is weakly expressed in H. disjuncta PCC 6712 but prominent in Hyella patelloides LEGE 07179. Thus, our study pinpoints crucial but hidden aspects of polyphasic cyanobacterial taxonomy.

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Expression from DIF1-motif promoters of hetR and patS is dependent on HetZ and modulated by PatU3 during heterocyst differentiation

Cited by 2 publications

References 43 publications

Interaction network among factors involved in heterocyst-patterning in cyanobacteria

Interaction network among factors involved in heterocyst-patterning in cyanobacteria

Final Destination? Pinpointing Hyella disjuncta sp. nov. PCC 6712 (Cyanobacteria) Based on Taxonomic Aspects, Multicellularity, Nitrogen Fixation and Biosynthetic Gene Clusters

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