1999
DOI: 10.1007/s004240050792
|View full text |Cite
|
Sign up to set email alerts
|

Expression environment determines K + current properties: Kv1 and Kv4 α-subunit-induced K + currents in mammalian cell lines and cardiac myocytes

Abstract: Voltage-gated K+ channel (Kv) pore-forming (alpha) subunits of the Kv1 and Kv4 subfamilies have been cloned from heart cDNA libraries, and are thought to play roles in the generation of the transient outward K+ current, Ito. Heterologous expression of these subunits in Xenopus oocytes, however, reveals K+ currents that are quite distinct from Ito. In the experiments here, the detailed time- and voltage-dependent properties of the currents expressed in mammalian cell lines and in cardiac myocytes by Kv1.4 and K… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

7
31
0

Year Published

2000
2000
2005
2005

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 63 publications
(38 citation statements)
references
References 37 publications
(116 reference statements)
7
31
0
Order By: Relevance
“…The subunit composition of Kv channels influences the expression, and electrophysiological and pharmacological properties of the associated currents (Hopkins, 1998). Kv channel expression and function may be modulated not only by coassociation of the various pore-forming ␣-subunits with accessory (␤)-subunits and other regulatory proteins but also by post-translational modifications of either the Kv ␣-or Kv ␤-subunits (Martens et al, 1999;Petersen and Nerbonne, 1999). Several K ϩ channel subunits are typically expressed in a single cell.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The subunit composition of Kv channels influences the expression, and electrophysiological and pharmacological properties of the associated currents (Hopkins, 1998). Kv channel expression and function may be modulated not only by coassociation of the various pore-forming ␣-subunits with accessory (␤)-subunits and other regulatory proteins but also by post-translational modifications of either the Kv ␣-or Kv ␤-subunits (Martens et al, 1999;Petersen and Nerbonne, 1999). Several K ϩ channel subunits are typically expressed in a single cell.…”
Section: Discussionmentioning
confidence: 99%
“…Cumulative inactivation is not apparent. have been impossible to adequately test this hypothesis by measuring only electrophysiological and pharmacological characteristics of I Kur , because 1) the voltage dependence, gating kinetics, and toxin sensitivity of heteromeric K ϩ channels are not easily predicted and vary considerably with expression environment (Hopkins, 1998;Petersen and Nerbonne, 1999); and 2) there are no specific antagonists of Kv1.5. Accordingly, we performed additional experiments with standard molecular and biochemical techniques, as detailed below.…”
Section: K ؉ Channel Diversity In Granulosa Cellsmentioning
confidence: 99%
“…Percentages (from the biotinylation cell surface assay) were subjected to arcsin transformation to produce data with a normal distribution (30) (31,32), no voltage-gated currents were detected in whole-cell recordings from cells transfected with pEGFP-C1 alone (mock-transfected; n ϭ 6) (Fig. 1A).…”
Section: Methodsmentioning
confidence: 99%
“…For example, recent reports have elegantly implicated I to in the mechanism of arrhythmias associated with Brugada syndrome, whereby phase 2 reentrant excitation could occur between cells with recovered I to and those with inactive I to (3,9). Both Kv4.3 and Kv1.4 proteins are expressed in the myocardium of large mammals (29). Flecainide and H 2 O 2 have been used to determine the contribution of these two K ϩ channel subunits to native I to (12,36,39).…”
Section: Discussionmentioning
confidence: 99%