Expression dynamics of vascular endothelial markers: endoglin and syndecan-1 in predicting dengue disease outcome

Mariappan, Vignesh; Adikari, Shalinda; Lokesh, S.; Easow, Joshy M; Pillai, Agieshkumar Balakrishna

doi:10.1016/j.trsl.2021.02.001

Cited by 16 publications

(11 citation statements)

References 50 publications

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“…For example, endoglin is an endothelial marker that is highly expressed during the inflammation process. The soluble form of endoglin (sEng) is released into circulation by activated cells and is reported to be potential markers for vascular leakage in dengue disease severity [ 101 , 102 ]. Similar to Eng, another important marker of vascular endothelial activations called SDC-1 is reported to induce neutrophil chemotaxis, inhibit alveolar epithelial wound healing, and promote pulmonary fibrosis [ 103 ].…”

Section: Endothelial Markersmentioning

confidence: 99%

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Mariappan

Manoharan

Ranganadin

et al. 2021

Microbial Pathogenesis

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The current pandemic due to the fast spreading of SARS-CoV-2 infection has caused severe impairment in health, social, economic, scientific, and medical sectors across the globe. Owing to the not so well understood mechanism of disease pathogenesis in terms of variations in immune responses, there remains obscure why some of the patients who are infected by the novel SARS-CoV-2 develop an unpredictable clinical course that rapidly causes severe and deadly complications/manifestations. Currently, several assays are available for the confirmation of SARS-CoV-2 infection at the point of care. However, none of these assays can predict the severity of the COVID-19 disease. Thus, the identification of a prognostic biomarker that forecasts the condition of SARS-CoV-2 patients to develop a severe form of the disease could enable the clinicians for more efficient patient triage and treatment. In this regard, the present review describes the role of selected biomolecules that are crucially involved in the immune-pathogenesis of SARS-CoV-2 infection such as hyper-immune responsiveness, bradykinin storm and vascular leakage assuming these may serve as an effective prognostic biomarker in COVID-19 to understand the outcome of the disease. Based on the review, we also propose the development of a cost-effective SERS-based prognostic biosensor for the detection and quantification of biomolecules for use as a point-of-care system during a disease outbreak.

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Section: Endothelial Markersmentioning

confidence: 99%

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Mariappan

Manoharan

Ranganadin

et al. 2021

Microbial Pathogenesis

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“…In dengue, impaired platelet aggregation to ADP from dengue antigen immune complexes formation on platelet surfaces have been reported [ 23 , 24 ]. A recent study analyzing the expression of endoglin (EDG) and Syndecan-1 (SDC1) from activated endothelial cells during plasma leakage in critical phase of dengue showed significant correlation in predicting dengue severity [ 25 ]. Hence, significant association of raised hematocrit with ROTEM abnormalities as found in this study has further potential for translational research in the future.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Dengue Warning Signs during Febrile Phase with Rotational Thromboelastometry, Cortisol and Feritin

Abas

Karim

Periyasamy

et al. 2022

IJERPH

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Dengue mortality remains high despite monitoring against warning signs (WS). The associations of WS at febrile phase (FP) and hemorrhage at defervescence with the levels and kinetics of ROTEM, platelet count, cortisol, and ferritin were analyzed. Patients with confirmed dengue serology and WS in two centers were screened (n = 275) and 62 eligible patients were recruited prospectively over 9 months. “Vomiting” was the commonest WS (62.9%), with shortened clotting time (CT) INTEM (p = 0.01). “Hematocrit increase” showed significant prolonged CT INTEM, EXTEM, and FIBTEM (p < 0.05). “Platelet decrease” showed reduced platelet function and reduced clot amplitude at 10 min (A10) and maximum clot firmness (MCF) in INTEM and EXTEM (p < 0.001). The kinetics were reduced in platelet count, CT EXTEM, and cortisol (p < 0.05) but increased in CT INTEM (p = 0.03). At FP, “vomiting”, “hematocrit increase”, and “platelet decrease” demonstrated impaired CT, clot strengths A10/MCF and platelet functions. Majority (60/62, 96.7%) had non-severe outcomes, consistent with increase in cortisol kinetics. In conclusion, “vomiting”, “hematocrit increase” and “platelet decrease” at FP correlated with ROTEM. No conclusion could be made further regarding ferritin and cortisol. Larger study is required to study “hematocrit increase” with ROTEM as a potential marker for hemorrhage.

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“…Moreover, many other molecules are released by activated ECs and have been strongly associated with severe plasma leakage in dengue infected patients. As such as a high level of syndecan-1, claudin-5, and chondroitin sulfate [ 58 , 59 ]. More recently, endoglin and syndecan-1 were quantified the mRNA expression and soluble protein levels in dengue cases during febrile, defervescence, and convalescence stages in DF, DFWS, and severe cases compared to non-dengue Other Febrile Illness (OFI) and healthy control.…”

Section: Discussionmentioning

confidence: 99%

Subsets of Cytokines and Chemokines from DENV-4-Infected Patients Could Regulate the Endothelial Integrity of Cultured Microvascular Endothelial Cells

et al. 2022

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Introduction: It is a consensus that inflammatory mediators produced by immune cells contribute to changes in endothelial permeability in dengue. We propose to relate inflammatory mediators seen in dengue patients with the in vitro alteration of endothelial cells (ECs) cultured with serum from these patients. Methods: Patients with mild (DF) to moderate and severe dengue (DFWS/Sev) were selected. ELISA quantified inflammatory mediators. Expression of adhesion molecules and CD147 were evaluated in the ECs cultured with the patient’s serum by flow cytometry. We assessed endothelial permeability by measuring transendothelial electrical resistance in cocultures of ECs with patient serum. Results: Dengue infection led to an increase in inflammatory mediators—the IL-10 distinguished DF from DFWS/Sev. There were no changes in CD31, CD54, and CD106 but decreased CD147 expression in ECs. DFWS/Sev sera induced a greater difference in endothelial permeability than DF sera. Correlation statistical test indicated that low IL-10 and IFN-γ and high CCL5 maintain the integrity of ECs in DF patients. In contrast, increased TNF, IFN-γ, CXCL8, and CCL2 maintain EC integrity in DFWS/Sev patients. Conclusions: Our preliminary data suggest that a subset of inflammatory mediators may be related to the maintenance or loss of endothelial integrity, reflecting the clinical prognosis.

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Expression dynamics of vascular endothelial markers: endoglin and syndecan-1 in predicting dengue disease outcome

Cited by 16 publications

References 50 publications

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Correlation of Dengue Warning Signs during Febrile Phase with Rotational Thromboelastometry, Cortisol and Feritin

Subsets of Cytokines and Chemokines from DENV-4-Infected Patients Could Regulate the Endothelial Integrity of Cultured Microvascular Endothelial Cells

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