Expression dynamics of HAND1/2 in in vitro human cardiomyocyte differentiation

Okubo, Chikako; Narita, Megumi; Inagaki, Azusa; Nishikawa, Misato; Hotta, Akitsu; Yamanaka, Shinya; Yoshida, Yoshinori

doi:10.1016/j.stemcr.2021.06.014

Cited by 10 publications

(12 citation statements)

References 58 publications

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“…RNA quality was assessed using an Agilent RNA6000 Pico Kit on a Bioanalyzer 2100 (Agilent). The library preparation and subsequent analysis were carried out following methods outlined in previous studies 53,54 . Briefly, 100 ng of DNase-treated total RNA was used for library preparation with the Illumina Stranded Total RNA Prep Ligation with Ribo- Zero Plus kit (Illumina).…”

Section: Methodsmentioning

confidence: 99%

Selective Translation Orchestrates Key Signaling Pathways in Primed Pluripotency

Okubo,

Nakamura,

Sato

et al. 2024

Preprint

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Pluripotent stem cell (PSC) identities, such as differentiation and infinite proliferation, have long been understood within the frameworks of transcription factor networks, epigenomes, and signal transduction, yet remain unclear and fragmented. Directing attention toward translational regulation, as a bridge between these events, promises to yield new insights into previously unexplained mechanisms. Our functional CRISPR interference screening-based approach revealed that EIF3D maintains primed pluripotency through selective translational regulation. The loss of EIF3D disrupts the balance of pluripotency-associated signaling pathways, impairing primed pluripotency. Moreover, we discovered that EIF3D ensures robust proliferation by controlling the translation of various p53 regulators, which maintain low p53 activity in the undifferentiated state. In this way, selective translation by EIF3D tunes the homeostasis of the primed pluripotency networks, ensuring the maintenance of an undifferentiated state with high proliferative potential. Therefore, this study establishes a paradigm for selective translational regulation as a defining feature of primed PSC identity.

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Section: Methodsmentioning

confidence: 99%

Selective Translation Orchestrates Key Signaling Pathways in Primed Pluripotency

Okubo,

Nakamura,

Sato

et al. 2024

Preprint

Self Cite

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“…Purified RNA samples were evaluated using an Agilent RNA6000 Pico Kit (Agilent) on a Bioanalyzer 2100 (Agilent). We performed the library preparation and subsequent analysis as described previously ( Okubo et al., 2021a , 2021b ). In brief, 100 ng of purified RNA was applied to the library construction using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (Illumina).…”

Section: Methodsmentioning

confidence: 99%

A stress-reduced passaging technique improves the viability of human pluripotent cells

Takahashi

Okubo

Nakamura

et al. 2022

Cell Reports Methods

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“…HAND1 plays key role in neural crest development. It also interacts with BMP4 which contributes further to the differentiation of cardiomyocytes [157,158]. It has major interactions with NKX2-5 and GATA4.…”

Section: Hand1/2mentioning

confidence: 99%

The benign nature and rare occurrence of cardiac myxoma as a possible consequence of the limited cardiac proliferative/ regenerative potential: a systematic review

Shafi

Siddiqui

Jaffry

2022

Preprint

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Background: Cardiac Myxoma (CM) is a primary tumor of heart. Its origins, why the occurrence of primary cardiac tumors is extremely rare and how it may be related to limited cardiac regenerative potential, these aspects are not yet entirely known. This study investigates the key cardiac genes/TFs and signaling pathways to advance our understanding of these important questions. Methods: Databases including PubMed, MEDLINE, and Google Scholar were searched for published articles without any date restrictions, involving cardiac myxoma, cardiac genes/TFs/signaling pathways and their roles in cardiogenesis, proliferation, differentiation, key interactions and tumorigenesis, with focus on cardiomyocytes. Results: The cardiac genetic landscape is governed by a very tight control between proliferation and differentiation-related genes/TFs/pathways. Cardiac myxoma originates possibly as a consequence of dysregulations in the gene expression of differentiation regulators including Tbx5, GATA4, HAND1/2, MYOCD, HOPX, BMPs. Such dysregulations switch the expression of cardiomyocytes into progenitor-like state in cardiac myxoma development by dysregulating Isl1, Baf60 complex, Wnt, FGF, Notch, Mef2c and others. The Nkx2-5 and MSX2 contribute to both proliferation and differentiation of Cardiac Progenitor Cells (CPCs) may possibly serve roles based on the microenvironment and the direction of cell circuitry in cardiac tumorigenesis. The Nkx2-5 in cardiac myxoma may serve to limit progression of tumorigenesis as it has massive control over the proliferation of CPCs. The cardiac cell type-specific genetic programming plays governing role in controlling the tumorigenesis and regenerative potential. Conclusion: The cardiomyocytes have very limited proliferative and regenerative potential. They survive for long periods of time and tightly maintain the gene expression of differentiation genes such as Tbx5, GATA4 that interact with tumor suppressors (TS) and exert TS like effect. The total effect such gene expression exerts is responsible for the rare occurrence and benign nature of primary cardiac tumors. This prevents the progression of tumorigenesis. But this also limits the regenerative and proliferative potential of cardiomyocytes. Cardiac Myxoma (CM) develops as a consequence of dysregulations in these key genes which revert the cells towards progenitor-like state, hallmark of CM. The CM development in carney complex also signifies the role of TS in cardiac cells.

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Expression dynamics of HAND1/2 in in vitro human cardiomyocyte differentiation

Cited by 10 publications

References 58 publications

Selective Translation Orchestrates Key Signaling Pathways in Primed Pluripotency

Selective Translation Orchestrates Key Signaling Pathways in Primed Pluripotency

A stress-reduced passaging technique improves the viability of human pluripotent cells

The benign nature and rare occurrence of cardiac myxoma as a possible consequence of the limited cardiac proliferative/ regenerative potential: a systematic review

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